ABSTRACT
Objective:To analyze and compare the volatile oil components of pine needle of Pinus yunnanensis from different habitats. Method:The volatile oils of pine needle of P. yunnanensis from 12 different habitats were extracted by supercritical CO2 extraction （SFE-CO2），the components were analyzed by gas chromatography-mass spectrometry （GC-MS），and relative percentage of each component was determined by peak area normalization method，and SPSS 20.0 software was used for principal component analysis. Result:The 137 compounds were isolated from the volatile oil of pine needle of P. yunnanensis from 12 different producing areas，with 1 sample identified 57 species，2 samples identified 57 species，3 samples identified 54 species，4 samples identified 48 species，5 samples to identify 58 species，6 samples identified 60 species，7 samples identified 53 species，8 samples identified 55 species，9 samples identified 56 species，10 samples to identify 58 species，11 samples identified 54 species and 12 samples to identify 50 species，composed of hydrocarbon，acid，alcohol，ester，aldehyde，ketone，phenol，ether 8 compounds，including 11 common composition including alpha pinene，beta pinene，beta caryophyllene，7C，10C，16C-hexadecatrienoic acid and palmitic acid methyl ester，palmitic acid，phthalic acid dibutyl do acid，alcohol，stearic acid，mountain，wood tar acid. Conclusion:In this study，supercritical CO2 extraction （SFE-CO2） was used to extract the volatile oil of pine needle of P. yunnanensis from different habitats. It was found that the volatile oil of pine needle of P. yunnanensis was mainly composed of hydrocarbons，acids，esters and alcohols. And the content and species of volatile oil of pine needle of P. yunnanensis from different producing areas differ greatly which lay a foundation for the further development and utilization of pine needle of P. yunnanensis resources.
ABSTRACT
XI Hong, a famous acupuncturist in the Southern Song Dynasty, has an important influence and status in history of Chinese acupuncture and moxibustion. After he moved to Linchuan, Jiangxi province, his descendants passed acupuncture from generation to generation. According to records, it was passed to 12 generations. After the tenth generation, it was also passed to apprentices besides descendants, such as accomplished acupuncturists CHEN Hong-gang, LIU Jin and others, forming XI Hong acupuncture school of thought. The poem of acupuncture and moxibustion XI Hong Fu is the representative work about XI Hong academic thought, which was additionally compiled or written by XI Hong's apprentices according to XI Hong academic thought. The poems are of characteristics in acupuncture application and association of acupoints. The academic thought and therapeutic methods of acupuncture in the poems still are widely used in modern clinical acupuncture practice.