ABSTRACT
AIM: To investigate the mechanism of baicalin-induced apoptosis in human laryngeal cancer cells. METHODS: AMC-HN-8 cells were selected for the study, and baicalin was applied to the cells at different concentrations (0, 10, 30, 100, and 300 μmol/L), and the half-inhibitory concentration (IC50) was measured by the CCK-8 method. Bax, cleaved-caspase-3, Cyto-c, IRF4 protein expression by protein blotting (Western blot); miR-125b-5p and IRF4 expression by RT-qPCR. Dual-luciferase reporter gene validation of Targetscan prediction (binding of miR-125b-5p to IRF4-3'UTR); apoptosis and necrosis inhibitors explore the way baicalein induces death in laryngeal cancer cells. AMC-HN-8 was then divided into blank group, baicalein (IC50), miR-125b-5p inhibitor group, baicalein + inhibitor NC group, baicalein+miR-125b-5p inhibitor group, and cell invasion and clone formation assays to detect cell invasion and proliferation ability, respectively. Apoptosis was detected by flow cytometry. RESULTS: Baicalein inhibited the proliferation of AMC-HN-8 cells in a dose-dependent manner with an IC50 value of 47.31 μmol/L. Compared with the blank group, 47.31 μmol/L baicalin induced apoptosis and inhibited cell invasion, while upregulating the expression of miR-125b-5p and suppressing the mRNA and protein levels of IRF4. The luciferase results showed that the miR-125b-5p mimic was able to inhibit the activity of the IRF4-3'UTR promoter relative to the NC mimic (mimic) group. Baicalein induces laryngeal cancer cell death in an apoptotic manner. In addition, the combination of 47.31 μmol/L baicalin and miR-125b-5p inhibitor affected the behavior of AMC-HN-8 cells, showing that compared with the blank group, the baicalin group showed a decrease in the number of cell clones, weakened invasion ability, and increased apoptosis; the miR - 125b-5p inhibitor group showed an increase in the number of cell clones, enhanced invasion ability and decreased apoptosis. The baicalin+ inhibitor NC group was consistent with baicalin, with no significant effect of inhibitor NC on cell behavior. The cloning, invasion, and apoptosis of cells in the baicalin+miR-125b-5p inhibitor group were intermediate between the baicalin and miR-125b-5p inhibitor groups. CONCLUSION: Baicalin inhibits the proliferation of AMC-HN-8 cells, and the mechanism may be related to miR-125b-5p targeting to inhibit the expression of IRF4, inducing the pro-apoptotic proteins Bax, cleaved-caspase3, and Cyto-c, and inhibiting the apoptosis suppressor protein Bcl-2 thereby inducing apoptosis.
ABSTRACT
Objective To investigate the whether miR‐125b expression having the abnormality in nasopharyngeal carcinoma tissues and serum and whether having the correlation with the sensitivity of cisplatin chemotherapy .Methods The intact medical records and tissue and serum samples in 34 cases of clinically diagnosed nasopharyngeal carcinoma were collected and the serum samples in 34 individuals undergoing the physical examination were also collected as the controls .The expression of miRNA‐125b in carcinoma tissue ,paracancerous tissue and serum was detected by real‐time quantitative polymerase chain reaction (real‐time qPCR) .The statistical analysis was performed .Results The expression of miRNA‐125b in carcinoma tissue was significantly lower than that in paracancerous tissue(P=0 .006) ,and serum miRNA‐125b expression in nasopharyngeal carcinoma patients was signifi‐cantly lower than that in the individuals undergoing the healthy physical examination(P=0 .000);but the miRNA‐125b expression had no correlation between the paracancerous tissue and serum(r=0 .112 ,P=0 .528) .The expression of miRNA‐125b in cancer tis‐sues was correlated with the pathological typing ,T staging and N staging(P0 .05) .Conclusion The low miRNA‐125b expression may be involved in the occurrence and development of nasopharyngeal carcinoma and plays a certain significance for the diagnosis ,pathology typing and classification of nasopharyngeal carcinoma .Its expression level in cancer tissue can serve as one of indicators for screen‐ing out the cisplatin chemotherapy sche.
ABSTRACT
Objective To study the causes, temporal courses, predictors and outcomes of delayed neurological deterioration (DND) after mild traumatic brain injury. Methods In a cohort of 254 patients, the incidence, causes and temporal courses of DND after mild traumatic brain injury were summed up. Univariate and multivariate (Logistic regression) analysis were performed to assess the predictors of DND. The short-term outcomes were compared between patients with and without DND. Results DND occurred in 14.2% (36/254) of the 254 patients. Intracranial causes of DND such as hematoma growth were observed in 91.7%(33/36) of patients with DND. The interval from arrival to DND was 0.5- 59.0 h. Thirty patients (83.3%, 30/36) deteriorated within 24 h. The univariate analysis revealed that dangerous injury mechanism, loss of consciousness, severe headache, more than 1 time vomiting, interval from injury to first CT scanning, first Glasgow coma scale (GCS) scores, basal skull fracture, hypocoagulability, heavy alcohol consumption were the predict factors for DND (P<0.05 or<0.01). The multivariate analysis revealed that interval from injury to first CT scanning (P=0.000, OR=0.169, 95% CI: 0.083- 0.344), first GCS scores (P = 0.004, OR = 0.355, 95%CI: 0.175- 0.721) and hypocoagulability (P=0.001, OR=0.077, 95%CI:0.017-0.354) were the independent predict factors for DND. All of the patients without DND recovered better. But in the patients with DND, 30 patients recovered better, 4 patients had slight disability, 1 patient had severe disability, and 1 patient died. The treatment outcome between two groups had significant difference (Z =-6.088, P = 0.000). Conclusions The causes of DND are mostly intracranial. Most patients deteriorat within 24 h. The interval from injury to first CT scanning, first GCS scores and hypocoagulability appear to be independent predictors of DND. DND is associated with poor short-term outcomes.
ABSTRACT
Objective To study the characteristics of intramedullaiy tumors of embryonal origin, surgical treatment and outcome. Methods The data of 26 cases with intramedullary tumors of embryonal origin, including clinical features, pathological types and imaging characters of the tumor, surgical treatment and outcome, were retrospectively summarized. Among the 26 patients, apart from the neurological symptoms, lower-extremity deformities were seen in 7 cases, scoliosis in 1 case, lower midline cutaneous abnormalities in 3 cases. The tumors involving lumber section of the spinal cord (thoracolumbar + lumbar segments + lumbosacral) in 21 cases, account for 80.8% (21/26). The tumor in different kinds had its characteristic MRI appearance. All of the 26 patients underwent surgical treatment. Subtotal resection of the tumor was performed in 19 cases, most partial resection in 5 cases, partial removal in 2 cases. Results Of the 26 patients, 25 patients had improved neurologically when leaving hospital. Twenty-one patients were followed up from 6 to 82 (30.0 ± 8.2) months, 12 patients had a good recovery without any neurological defect, 7 patients could live independently, 1 patient needed care offered by others, 1 patient with malignant teratoma died at 6 months after operation. In the follow-up period, no patient needed the second surgery for recurrent tumor. Conclusions Apart from the neurological symptoms, other abnormalities such as lower-extremity deformities, scoliosis, lower midline cutaneous abnormalities could be appeared in patients with intramedullary tumors of embryonal origin. Most tumors involving lumber section of the spinal cord, could be diagnosed by MRI. Other spinal abnormalities frequently occur with the tumors. It is difficult to remove the tumors totally, while the surgical outcome is satisfactory.
ABSTRACT
Objective To study the value of configuration of the fourth ventricle to indicate, surgical treatment in patients with spontaneous cerebellar hemorrhage. Methods The data of 52 patients with spontaneous cerebellar hemorrhage were retrospectively analyzed. The relationship between configuration of the fourth ventricle and consciousness, volume of the hematoma, frequence of hydrocephalus and incidence of deterioration of consciousness was investigated. Results The correlations between the degree of the fourth ventricular compression and the consciousness, the volume of the hematoma were strong (r= -0.881,P <0.01; r=0.808,P < 0.01, respectively). The more the degree of the fourth ventricular compression was severe,the more GCS score was low and the volume of the hematoma was large. The incidence of hydrocephalus in patients with composed the fourth ventricle[57.7%(15/26)] was significantly higher than that in patients with normal configuration of the fourth ventricle [16.7% (3/18)] (χ~2= 7.406, P < 0.01). The incidence of deterioration of consciousness in patients with compressed the fourth ventricle who accepted conservative therapy initially [72.7% (8/11)]was significantly higher than that in patients with normal configuration of the fourth ventricle [12.5%(2/16)] (P= 0.003). Conclusions The configuration of the fourth ventricle is an useful indicator for surgical treatment in patients with spontaneous cerebellar hemorrhage. When making decision whether surgical treatment was used or not in patients with spontaneous cerebellar hemorrhage, the configuration of the fourth ventricle should be take into account.