ABSTRACT
Objective@#To investigate the effects CXCL1 derived from TAMs on the progression of HCC by CXCL1 in tumor microenvironment (TME) .@*Methods @#PMA induced human monocyte (THP-1) to obtain undifferentiated macrophages (M0) and then co-cultured with Huh 7 HCC cells.The biomarkers of macrophage phenotype and mRNA level of HCC were analysed by qRT-PCR. Colony formation assay,cell viability assay and transwell assay were performed to evaluate the biological alteration of HCC cells in TME.Epithelial-mesenchymal transition (EMT) molecular genetics were detected using western blot.The levels of CXCL1 in TME were measured by ELISA assay. @*Results @#High CXCL1 expression in HCC patients predicted poor prognosis.Abundant macrophages were found in CXCL1 high expression HCC tissues.In macrophages and HCC co-cultured model,significantly increasing CXCL1 expression was detected in both two cells and the biomarkers of M2 macrophages,CD163 and CD206 were elevated.The growth and migration of HCC cells were promoted.@*Conclusion @#Co-culture of macrophages with HCC cells induces macrophages pro-tumor phenotype and CXCL1 secretion which promotes the progression of HCC.