ABSTRACT
To identify the gene differentially expressed in female Anopheles anthropophagus and to analyze its gene sequence, this gene amplified by PCR was identified by real-time PCR and its cDNA was then amplified with rapid amplification of cDNA ends (RACE) technology. It was found that the expression ratio of the female differentially expressed gene in female and male mosquitoes was 267.49 according to the formula F=2~(-⊿⊿CT).The size of mRNA of the gene was 364 bp, and the amino acid sequence deduced from the open reading frame (ORF) was found to be similar to the sequence of tectin protein of Culex quinquefasciatus and proteins of other species. The mRNA sequence of this gene was submitted to NCBI with a accession number of FJ907236. This gene may provide a foundation for further studies on the biological functions of mosquitoes.
ABSTRACT
Objective:To study the relationship between QT dispersion and severe ventricular arrhythmias.Methods:of 128 patients diagnosed as coronary heart diseasec (CHD), the severe ventricular arrhythmiae was detected by electrocardiogram in 48 patients and the unsevere ventricular arrhythmias was detected in 46 patients. Their QTd were measured and compared with the control group 34 patients with wronary heart disease, but without ventricular arrhythmias.Results:QTd was especially prolbyged in severe ventricular arrhythmia group than those in control group (P