Correlation analysis between psychological status and blood pressure of surrounding residents and employees in a medium-risk area during COVID-19 epidemic / 中华健康管理学杂志

Objective:To explore the correlation analysis between psychological status and blood pressure of residents in medium-risk areas during the coronavirus disease 2019 (COVID-19) epidemic, and provide references for early psychological intervention and hypertension management during the COVID-19 epidemic.Methods:From January 4 to 8, 2021, the convenience sampling method was used to conduct psychological questionnaire surveys on residents and employees of enterprises in the medium-risk area and surrounding areas of Jiuxianqiao, Chaoyang District, Beijing. General data (gender, age, height, weight, history of smoking and drinking, previous medical history), and home blood pressure monitoring were collected. Pearson′s χ 2 test was used to compare different factors (anxiety, depression, age, gender, body mass index, smoking, drinking, and history of chronic diseases) in normal blood pressure or not. A multivariate logistic regression analysis model was established to explore independent risk factors of abnormal blood pressure. Results:No anxiety accounted for 75% (684/912), and anxiety accounted for 25% (228/912). No depression accounted for 67% (611/912), depression accounted for 33% (301/912). Both anxiety and depression accounted for 23% (210/912). The substandard blood pressure accounted for 10.3% (94/912). The incidence of poor blood pressure control in participants with anxiety was 30.7% (70/228), which was significantly higher than that of participants without anxiety 3.5% (24/684) (χ 2=136.779, P<0.001). The incidence of poor blood pressure control in participants with depression 27.2% (82/301) was significantly higher than that of participants without depression 2.0% (12/611) (χ 2=139.388, P<0.001). Multivariate logistic regression analysis showed anxiety ( OR=3.261, P<0.001), depression ( OR=7.928, P<0.001), and complications of chronic diseases ( OR=3.207, P<0.001) were closely related to whether blood pressure was under control. Conclusions:During the COVID-19 epidemic, the psychological status of residents and employees in medium-risk areas should be screened and interventions implemented accordingly to prevent unstable blood pressure caused by anxiety or depression.

Association of beta-adrenoceptor gene polymorphism with resting heart rate / 生物医学工程学杂志

This study was aimed to shed light on the association of beta-adrenoceptor (beta-AR, includeing three subgroups: beta1, beta2 and beta3)gene polymorphism with resting heart rate (RHR). The RHRs of 150 healthy subjects (male 80; female 70) in the supine were detected by computerized system for analyzing the electrocardiosignals. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and allele specific-PCR techniques were used to determine Ser49Gly and Arg389Gly polymorphism of beta1-adrenoceptor gene; Arg16Gly and Gln27Glu polymorphism of beta2-adrenoceptor gene and Trp64Arg polymorphism of beta3-adrenoceptor gene; in these subjects. The results were as follows: The Arg389Gly polymorphism of beta1-adrenoceptor gene is significantly associated with RHR (P<0. 05), and the difference among three RHRs in male group is significantly higher than in group female group (P=0.0030 vs 0.0045). Individuals with Gly/Gly genotype have the highest RHR (male: 80.98+/-3.09; female: 84.23+/-6.28). No significant association was found among Ser49Gly, Arg16Gly, Gln27Glu and Trp64Arg polymorphism with RHR respectively (P> 0.05). RHR is associated with genotype. Arg389Gly polymorphism of beta1-adrenoceptor gene is significantly associated with RHRs, and the higher significant difference among male's RHRs,when compared with that among female's, suggests that male's RHRs be more influenced by genotype. Thus the clinical phenomenon of "the correlation of heart rate and mortality in male is higher than that in female" could be explained at the gene level.

Analysis on time-frequency feature of rabbit cortical somatosensory evoked potential / 生物医学工程学杂志

This experiment was carried out to analyze the time-frequency feature of rabbit cortical somatosensory evoked potential (SEP). Rabbit was narcotized and subjected to craniotomy. SEP was from sensory and motor cortex. Stimulation was continuing many times and signal was sampled at 3 800 Hz. The peak latency of each waveform was measured. Power spectrum of SEP was analyzed. The time-frequency feature of single-trial was compared with that of average SEP. It was found that the variability of single-trial SEP latency enlarges with time in a stimulation period. The spectrum of SEP includes three main frequency spectrum packages. The technique of summation makes a lot of signal aberration such as waveform confluence, new waveform emerging and after-discharging components dismissing.

Genotype combination distribution of 5-site SNPs in 3 subtypes of beta adrenoceptor / 生物医学工程学杂志

DNA was extracted from the peripheral venous blood of 338 subjects using BLOOD DNA MINI KIT. The 5 site SNP in 3 subtypes of Beta-AR were genotyped by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) and allele-specific primer PCR techniques. The genotypes combination distribution of SNP at 5 sites in the 3 subtypes of Beta-AR were determined by clustering analysis technique. The natural combination distribution characteristics for SNP at 5 sites in the 3 subtypes of Beta-AR in 338 subjects were obtained. Sixty-seven combinations types were found. The preceding 5 combinations in the natural combination distribution of the SNP were: (1) The genotype combination of forty subjects was B1-AR S/S49+B1-AR R/R389+B2-AR R/G16+B2-AR Q/E27+B3-AR W/W64, its probability was 11.83%. (2) The genotype combination of thirty-three subjects was B1-AR S/S49+B1-AR R/R389+B2-AR R/G16+B2-AR Q/Q27+B3-AR W/W64, its probability was 9.76%. (3) The genotype combination of nineteen subjects was B1-AR S/S49+B1-AR R/G389+B2-AR R/G16+B2-AR Q/Q27+B3-AR W/W64, its probability was 5.62%. (4) The genotype combination of sixteen subjects was B1-AR S/S49+B1-AR R/G389+B2-AR R/G16+B2-AR Q/E27+B3-AR W/W64, its probability was 4.74%. (5) The genotype combination of thirteen subjects was B1-AR S/G49+B1-AR R/R389+B2-AR R/G16+B2-AR Q/E27+B3-AR W/W64, its probability was 3.85%. The obvious correlations exist among full sample and female or male subgroup, and between female and male subgroups.

Separating independent components in heart period signal / 生物医学工程学杂志

To extract sub-signal of heart period signal (HPS), a new statistical signal processing approach, namely independent component analysis (ICA) was addressed. Electrocardiosignal (ECS) was acquired from ten volunteers. ECS was sampled 8 minutes when the volunteer was in supine position, and then when the same volunteer was in erect position. HPS was extracted from ECS. According to time-delay, HPS was divided into five groups as mixed signals. Five signals were reconstructed into two groups by ICA. The rebuilt signals were transformed by Fourier transformation. One centralized in low frequency (called IC1); the other did in high frequency (called IC2). The power of IC1 was significantly increased (P<0.01) while that of IC2 showed no significant change (P>0.05), and the ratio of IC1 to total power also significantly increased with the change from supine position to erect position. Comparsion between the two postural results reveals that IC1 may express sympathetic activity, and IC2 represents parasympathetic activity. Sympathetic and parasympathetic nervous functions can be evaluated respectively and quantitatively by use of data and graphs from the two decomposed components.

Analyzing heart period signal for fetus and pregnant woman in perinatal period / 生物医学工程学杂志

Analyzing the digital characteristics (chaotic and spectral features) of fetal and pregnant woman's heart period signal (HPS) can assess the autonomic nervous system function. Extracting and analyzing HPS of fetal and pregnant woman in perinatal period were realized by using the method of visual programming. The subjects were in supine position. Electrocardiography-signal (ECS) from the leads placed at symphysis pubic bone to inferior border of abdominal wall was acquired. ECS was preprocessed by wavelet filter. The sophisticated technique developed by our laboratory was used to analyze the digital characteristics of HPS. The system could be used to assess fetal and pregnant woman's autonomic nervous system function, furthermore, sympathetic and parasympathetic nervous function could be evaluated respectively and quantificationally. The system also could be used to prognosticate fetal distress. The digital characteristics of fetal and other age groups' HPS, which vary with age, suggest the physiological process of development, mature and senility of autonomic nervous system; based on it, we could find the way of anti-senility. Some digital parameters of fetal HPS stand between those of newborn and adult, which may imply that fetal autonomic nervous regulation is influenced by mother's nerve and endocrine system.

Effects of Betoloc on Autonomic Nervous System in the Heart / 中国药房

OBJECTIVE:To study the effects of Betoloc(metoprolol)on autonomic nervous system in the heart METHODS:Using the computerized analysis system developed by our laboratory,the characteristic parameters of heart period signal(HPS)from volunteers were examined before and 3 hours after administrating Betoloc(50mg,po ) RESULTS:Betoloc could increase the mean heart period,standard deviation of heart period,high frequency components and total power of HPS'spectra,and decrease heart rate,low frequency components significantly CONCLUSION:(1)Betoloc can increase standard deviation of heart period or heart rate,which means increasing irregularity of heart rate It is the basic reason of increasing high frequency components of HPS'spectra and is also the basic reason of increasing cholinergic nervous activity;(2)Betoloc can decrease tension of sympathetic nerves,increase tension of parasympathetic nerves,and increase tension of autonomic nervous system in the heart

Effect of Atropine on Cardiac Autonomic Nervous System / 中国药房

OBJECTIVE:To study the effects of atropine on cardiac autonomic nervous system.METHODS:Using the computerized analysis system developed by our laboratory,the characteristic parameters of heart period signal(HPS)in volunteers were examined before and 1 hour after administrating atropine(0.04mg iv).RESULTS:Atropine could decrease the mean heart period,standard deviation of heart period,high frequency components and total power of HPS spectra,and increase heart rate,relative power of ultra-low frequency components significantly.CONCLUSION:(1)Atropine can decrease standard deviation of heart period or heart rate,which means decreasing irregularity of heart rate.It is the basic reasons why high frequency components of HPS spectra increases,and parasympathetic nervous activity decreases,(2)Atropine can decrease the tonus of parasympathetic nerves,increase the tonus of sympathetic nerves relatively,and decrease the tonus of autonomic nervous system that reveals the decrease of the total power of HPS significantly.