ABSTRACT
Objective:To establish a TLC identification method for three medicinal ingredients and a GC method for the content determination of metnthol in Baihua Dingchuan tablets. Methods:Citrus Reticulata, Ephedra Sinica and Schisandra Chinensis were i-dentified by TLC. GC was used to determine the content of menthol in Baihua Dingchuan tablets. The chromatographic conditions were as follows:a DM-5 capillary column(30 m × 0. 53 mm, 1. 5 μm) was adopted. The injection port temperature was 200℃. An FID was used as the detector and the temperature was 300℃. The temperature program was as follows:the initial column temperature was 80℃, and then rose to 120℃ at the rate of 8 ℃· min-1 , finally rose to 185 ℃ at the rate of 15 ℃· min-1 and maintained for 10 min. The carrier gas was nitrogen and the flow rate was 3. 0 ml·min-1 . The sample size was 1 μl. Split injection was performed with the diversion ratio of 1 :5. Results: The three traditional Chinese medicines could be identified by TLC. The content of menthol showed a good linear relationship within the range of 1. 484-74. 200μg · ml-1 ( r =0. 9998 ) by GC. The average recovery was 97. 76% RSD=0 . 58%(n=9). Conclusion:The methods are specific and simple with good reproducibility. They can be used for the quality control of Baihua Dingchuan tablets.
ABSTRACT
Objective:To establish a TLC identification method for three medicinal ingredients and a GC method for the content determination of metnthol in Baihua Dingchuan tablets. Methods:Citrus Reticulata, Ephedra Sinica and Schisandra Chinensis were i-dentified by TLC. GC was used to determine the content of menthol in Baihua Dingchuan tablets. The chromatographic conditions were as follows:a DM-5 capillary column(30 m × 0. 53 mm, 1. 5 μm) was adopted. The injection port temperature was 200℃. An FID was used as the detector and the temperature was 300℃. The temperature program was as follows:the initial column temperature was 80℃, and then rose to 120℃ at the rate of 8 ℃· min-1 , finally rose to 185 ℃ at the rate of 15 ℃· min-1 and maintained for 10 min. The carrier gas was nitrogen and the flow rate was 3. 0 ml·min-1 . The sample size was 1 μl. Split injection was performed with the diversion ratio of 1 :5. Results: The three traditional Chinese medicines could be identified by TLC. The content of menthol showed a good linear relationship within the range of 1. 484-74. 200μg · ml-1 ( r =0. 9998 ) by GC. The average recovery was 97. 76% RSD=0 . 58%(n=9). Conclusion:The methods are specific and simple with good reproducibility. They can be used for the quality control of Baihua Dingchuan tablets.
ABSTRACT
OBJECTIVE@#To investigate the clinical efficacy of revision surgery of nasal septum with reformed incision under nasal endoscope.@*METHOD@#Thirty-seven patients with failed septoplasty were carried out revision surgery of nasal septum with reformed incision.@*RESULT@#Revision surgery of nasal septum with reformed incision was successful in all cases. The symptoms resulting from the nasal septal deviation disappeared or significantly relieved. Following successful revision surgery, the treatment outcomes of concomitant nasal and/or sinusal diseases also significantly improved.@*CONCLUSION@#The adhesive fibrous tissue in septal mucosa were successively separated in patients with revision surgery of nasal septum with reformed incision under nasal endoscope. Revision surgery of nasal septum with reformed incision was easily and safely, and with fewer complications.