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Objective To study the changes of the transient outward K+ current (Ito),delayed rectifier K+ current(IkDR) and the inward rectifier K+ current(Ik1) of the rat bone mesenchymal stem cells (MSCs) induced by 5-Azacytidine(5-Aza) during proliferating and differentiating process in vitro. Methods MSCs were cultured according to related articles for two weeks and then some of the cells were induced by 5-Azacytidine.The experiment was divided into uninduced(cultured for 6w) and induced(1,2,3 and 4w) cell groups. Each week had twenty cells random tested by the whole-cell patch clamp technology and the K+ currents were identified by corresponding ionic blockers.Results The detection rate of the same type of K+ current during test weeks had no significant deviation;The three types of K+ current intensity were gradually augmented after being inducing cultured for 1,2,3 and 4 weeks(P
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The internal medicine of paediatrics is the important major course in paediatrics. Focusing on strengthening the constructionof staff, we cultivate all levels of teachers from different administrative level and personality, do schorlarly research meticulously,standardizing management and improve the teaching evaluation system. Besides we promote the construction and development of theinternal medicine of paediatrics by carrying out teaching research, impsoving teaching methods and making paediatics the key subjectin China.
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Objective To investigate the relationship between day and night regularity of asymptomatic ventricular presystole (VPS) and the activity of autonomic nervous system and its clinical significance Methods Thirty children with the absence of underlying organic heart disease were examined with 24 hour dynamic electrocardiogram (DCG), the function of autonomic nervous system and ventricular late potentials (VLP) Results The rate (43%, 13/30) was the day type VPS, 12 out of the 13 resulted from increased activity of sympathetic nerve The rate (30%, 10/30) was the night type VPS, all of them resulted from the activity of the vagus nerve enhanced There was a positive correlation between VPS frequency of day type and heart rate ( r =0 67, P
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Objective The study is to evaluate autonomic nervous activity and its circadian rhythm over a 24 hour period in children with beta adrenergic receptor (? AR) hypersensitivity and hyperthyroidism by analysis of heart rate variability (HRV), and to discuss the diagnosis value of autonomic nervous tone change in children with ? AR hypersensitivity Methods Indexes of HRV are measured in 23 children with ? AR hypersensitivity, and 13 hyperthyroidism and 26 healthy control Time domain indexes and spectral indexes of HRV are derived and the night day difference of HRV is analyzed Results 1 Spectral indexes over a 24 hour period: In patients with ? AR hypersensitivity and hyperthyroidism, there are significant increases both in low frequency (LF) to high frequency (HF) ratio (LF/HF) and HF normalized units (HF UN ) compared with the control group Whereas there are no significant changes compared with ? AR hypersensitivity and hyperthyroidism 2 Spectral indexes during waking hours: In patients with? AR hypersensitivity and hyperthyroidism, there are no significant difference in LF normalized units (LF UN ), HF UN and LF/HF; whereas there are significance changes compared with normal subject 3 Spectral indexes during sleeping hours: there are no significantly differences in indexes of HF, LF UN and HF UN compared with ? AR hypersensitivity and normal subject, whereas these indexes significance changes compared with hyperthyroidism 4 Time domain indexes change over a 24 hour period: There are significant changes of the night day difference in time domain indexes with? AR hypersensitivity or control group, in which rMSSD and pNN50 are most sensitive indexes Whereas there are significant difference in patients with hyperthyroidism in any time domain indexes of HRV compared with control group or ? AR hypersensitivity, and the night day difference in HRV is significantly smaller compared with ? AR hypersensitivity or control group, the circadian rhythm is almost disappeared Conclusion It is showed that the children with? AR hypersensitivity have no difference from normal subjects in autonomic nervous system activity, but these patients respond differently to the catecholamine stimulus, which results in changes in autonomic balance, and relatively increase of sympathetic nervous activity But cardiac autonomic nervous activity still has a circadian rhythm, which is similar to normal subject The analysis of HRV may play an important role in diagnosis? AR hypersensitivity
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Transesophageal atrial pacing was performed for evaluation sino-atrial node function in 102 normal children aged 2-15 years. These cases were evaluation again to make a controlled study after autonomic nerve blockade with propranolol(0.1mg/kg)and atropin(0.02mg/kg). In the group, values of SCL, SNRT, CSNRT, SNRTI declined significantly after autonomic blockade comparing with that before(P