ABSTRACT
Objective To study the inhibitory effect of Rompun anesthesia and operation on immune function of dogs.Methods We randomly divided 14 healthy dogs into Group A and Group B with 7 in each.Rompun anesthesia induced by intramuscular injection of 3.0 mg/kg Rompun was carried out in Group A while Rompun anesthesia and operation were applied in Group B.The venous blood samples were collected before and after anesthesia at 2,8,24,72,120 and 168 h for centrifugation to obtain sera.ELISA kit was used to evaluate the contents of serum IL-1β,IL-2,IL-4,IL-8 and TNF-α.Results Compared with those before anesthesia,the concentrations of IL-2,IL-1βand TNF-αin Group A significantly decreased at 2,8,24 and 72 h (P0 .05 ).Compared with those before anesthesia,the concentrations of IL-2 and IL-1βin Group B significantly decreased from 2 to 168h (P<0.05),while the concentrations of TNF-αand IL-8 significantly increased (P<0 .05 ).IL-2 and IL-1βcontents in Group B from 2 to 168 h were significantly decreased compared with those in Group A (P<0.05 ),whereas IL-8 and TNF-αincreased significantly (P<0.05 ). Conclusion Both Rompun anesthesia and operation stress can inhibit dogs'immune function,and the latter has a stronger inhibitory effect than the former.
ABSTRACT
PURPOSE: Cathepsin K is a potent collagenase implicated in human and animal atherosclerosis-based vascular remodeling. This study examined the hypothesis that serum CatK is associated with the prevalence of coronary artery disease (CAD). MATERIALS AND METHODS: Between January 2011 and December 2012, 256 consecutive subjects were enrolled from among patients who underwent coronary angiography and percutaneous coronary intervention treatment. A total of 129 age-matched subjects served as controls. RESULTS: The subjects' serum cathepsin K and high sensitive C-reactive protein (hs-CRP) and high-density lipoprotein cholesterol were measured. The patients with CAD had significantly higher serum cathepsin K levels compared to the controls (130.8+/-25.5 ng/mL vs. 86.9+/-25.5 ng/mL, p<0.001), and the patients with acute coronary syndrome had significantly higher serum cathepsin K levels compared to those with stable angina pectoris (137.1+/-26.9 ng/mL vs. 102.6+/-12.9 ng/mL, p<0.001). A linear regression analysis showed that overall, the cathepsin K levels were inversely correlated with the high-density lipoprotein levels (r=-0.29, p<0.01) and positively with hs-CRP levels (r=0.32, p<0.01). Multiple logistic regression analyses shows that cathepsin K levels were independent predictors of CAD (odds ratio, 1.76; 95% confidence interval, 1.12 to 1.56; p<0.01). CONCLUSION: These data indicated that elevated levels of cathepsin K are closely associated with the presence of CAD and that circulating cathepsin K serves a useful biomarker for CAD.
Subject(s)
Aged , Female , Humans , Male , Middle Aged , Biomarkers/blood , C-Reactive Protein/metabolism , Cathepsin K/blood , Coronary Artery Disease/bloodABSTRACT
PURPOSE: Cathepsin K is a potent collagenase implicated in human and animal atherosclerosis-based vascular remodeling. This study examined the hypothesis that serum CatK is associated with the prevalence of coronary artery disease (CAD). MATERIALS AND METHODS: Between January 2011 and December 2012, 256 consecutive subjects were enrolled from among patients who underwent coronary angiography and percutaneous coronary intervention treatment. A total of 129 age-matched subjects served as controls. RESULTS: The subjects' serum cathepsin K and high sensitive C-reactive protein (hs-CRP) and high-density lipoprotein cholesterol were measured. The patients with CAD had significantly higher serum cathepsin K levels compared to the controls (130.8+/-25.5 ng/mL vs. 86.9+/-25.5 ng/mL, p<0.001), and the patients with acute coronary syndrome had significantly higher serum cathepsin K levels compared to those with stable angina pectoris (137.1+/-26.9 ng/mL vs. 102.6+/-12.9 ng/mL, p<0.001). A linear regression analysis showed that overall, the cathepsin K levels were inversely correlated with the high-density lipoprotein levels (r=-0.29, p<0.01) and positively with hs-CRP levels (r=0.32, p<0.01). Multiple logistic regression analyses shows that cathepsin K levels were independent predictors of CAD (odds ratio, 1.76; 95% confidence interval, 1.12 to 1.56; p<0.01). CONCLUSION: These data indicated that elevated levels of cathepsin K are closely associated with the presence of CAD and that circulating cathepsin K serves a useful biomarker for CAD.