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Osteoarthritis (OA), a chronic joint degenerative disease, is the significant cause of the loss of joint function in middle-aged and older people. Bone destruction, synovial hyperplasia, osteophyte formation, and subchondral bone sclerosis are the main features of osteoarthritis, and the typical symptom is severe joint pain. Consequent to unprecedented global population aging, osteoarthritis remains a leading cause of disability, and the treatment often comes at a high cost. With an in-depth understanding of related research, osteoarthritis is proven to be a multifactorial disease whose onset is not simply a cartilage lesion, and the immune plays an essential role in the development of the disease. Some studies proposed that chondrocytes are capable of altering gene expression and mediating osteoarthritis progression by regulating immune responses. Previous studies showed that the extent of immune dysregulation significantly correlates with the severity of osteoarthritis, revealing an association between immunity response and clinical manifestations. The study of immune infiltration, genetic alterations, and pathogenesis of osteoarthritis may provide new perspectives and methods for the treatment of osteoarthritis in the future. Therefore, this review, combined with the recent decade of literature, provides an overview of the research progress of the main immune cells and related cytokines in OA, which may provide a new direction of thinking for diagnosing and preventing this disease.
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Objective:To explore the effect of laparoscopic surgery on postoperative rehabilitation and stress response of patients with colorectal cancer.Methods:From January 2018 to September 2019, 94 patients with colorectal cancer were selected in the General Hospital of Marine Police of the Chinese People's Armed Police Force.They were divided into observation group (47 cases) and control group (47 cases) according to the random digital table method.The observation group was treated with laparoscopic surgery, while the control group was treated with open surgery.The changes of perioperative indicators, postoperative rehabilitation indicators, postoperative complications and 24-hour stress response indicators were compared between the two groups.Results:The time of lymph node dissection and operation had no statistically significant differences between the two groups (all P>0.05). The amount of bleeding of the observation group [(153.61±27.58)mL] was less than that of the control group [(271.32±23.25)mL] ( t=22.371, P<0.05). In the observation group, the exhaust time [(43.27±6.18)h] and the recovery time of bowel sounds [(20.31±3.28)h] were faster than those in the control group [(67.38±9.91)h and (34.54±5.17)h] ( t=14.153, 15.934, all P<0.05). The incidence of postoperative complications in the observation group (4.26%) was lower than that in the control group (21.28%) (χ 2=6.114, P<0.05). The serum levels of norepinephrine (NE) [(92.13±10.45)μg/L], C-reactive protein (CRP) [(17.38±3.01)mg/L] and Cor [(137.87±23.15)μg/L] in the observation group were lower than those in the control group [(136.27±15.42)μg/L, (26.73±2.34)mg/L and (185.42±27.39)μg/L] ( t=16.245, 16.813, 9.090, all P<0.05). Conclusion:Laparoscopic radical resection of colorectal cancer has good effect on patients with colorectal cancer.It can speed up postoperative rehabilitation and reduce stress response.It is worthy of popularizing.
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Objective To establish a stably overexpressing miR-31 transgenic mouse and detect the expression of miR-31 in the organs and tissues,and to provide qualified tool mice with overexpression of miR-31 in vivo. Methods The miR-31 overexpression vector was constructed by Gateway cloning technology. The vector was injected into fertilized ovum by DNA microinjection technology,then transferred to the pseudopregnant mice and waited for eutocia. Newborn mouse tail DNA was extracted and PCR and agarose gel electrophoresis were performed to identify the positive miR-31 transgenic mice. microRNA was extracted from the organs and tissues of miR-31 transgenic mice and the expression of miR-31 was de-tected by RT-PCR. The expression of Nestin and number of neural stem cells in the nervous system were compared in the positive and WT mice. Results The miR-31 transgenic mice were constructed successfully and bred more than 14 genera-tions in barrier environment. Expression of miR-31 was increased in major organs and tissues. The expression of Nestin and the number of neural stem cells in the positive mice were higher than those in the wild type mice. Conclusions MiR-31 overexpressing transgenic mice are constructed by Gateway cloning technology and the expression of miR-31 is stable in sub-sequent generations. The number of neural stem cells in the nervous system is higher than that in wild-type mice. The miR-31 overexpressing transgenic mice can be a good tool for experimental research of the function of overexpressed miR-31 in vivo and the treatment of nervous system diseases.
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Phthalate esters (PAEs) are commonly used plasticizers and have caused pollution to the environment due to their widespread use.Therefore, many countries have listed such compounds as a priority list of pollutants, and it is of great significance to establish an accurate analysis method for monitoring the pollution of PAEs in water.A method of solid phase membrane extraction combined with ultrasonic wave desortion-gas chromatography-mass spectrometer for analysis of PAEs in water was established in this study.The extraction and desorb conditions were optimized.In this study, the samples were ultrasonic wave desorbed for 7 min at bath temperature of 40℃ and ultrasonic powder of 50%.The detection limits (S/N>3) of this method were between 0.05 μg/L and 0.26 μg/L, the recoveries in different matrixes were between 76.2% and 112.3%, and the relative standard deviations were below 10%.
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Since the Beijing health and family planning commission promulgated the new medical technology management system,we have accepted and reviewed 14 key medical technologies in Beijing,such as resection of the skull base tumor (intracranial tumor outside communication),resection of intracranial important functional areas and large vascular malformation,renal vascular reconstruction technique,the technique of artificial joint replacement,coronary interventional diagnosis and treatment technology,etc.Through the existing laws and regulations requirements from medical new technology,the ethical review way,submit materials requirements and ethical review focus is analyzed,and combining with the experiences of our hospital in the medical technical review,aims to explore suitable for medical technology ethical review specification.
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Objective To investigate the effect of Liuwei Dihuang decoction combined with Taohong Siwu decoction in the treatment of traumatic renal contusion and laceration with long time no absorption of hematoma.Methods 166 patients with traumatic renal contusion and laceration with long time no absorption of hematoma from the department of urinary surgery from June 2014 to July 2016 in our hospital were selected and divided into two groups,83 cases in the control group treated with routine conservative treatment,83 cases in the experiment group received Liuwei Dihuang decoction combined with Taohong Siwu decoction on the basis of the control group,hematoma absorption,the scope of the hematoma of the kidney,the hemodynamic parameters of renal artery,the level of serum inflammatory factors,and the clinical effect were compared after the treatment.Results After treatment,the effective rate in the experiment group 93.98% was higher than the control group 77.11% (P<0.05);Compared with the control group,the beginning of absorption time of kidney hematoma,disappearance time of microscopic hematuria and the hospitalization time were shorter in the experiment group after treatment, the scope of perirenal hematoma was smaller after 2,4,12 weeks of treatment,the levels of Vmax and Vmin in renal aorta were higher,levels of AT and RI were lower after treatment, serum levels of TNF-α, IL-8, IL-1, IL-6 were lower after treatmentwith significant difference ( P <0.05 ). Conclusion Liuwei Dihuang decoction combined with Taohong Siwu decoction can significantly promote the absorption of hematoma in patients with traumatic renal contusion and laceration,improve the blood flow of renal artery,reduce the inflammatory reaction index, promote the recovery of renal injury,shorten the length of hospital stay.
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Artificial sweeteners ( ASs ) have gained more and more attention by environmental scientists because some of them such as acesulfame, have the potential to be the ideal tracers of domestic wastewater for environmental monitoring. In contrast to the existing methods of artificial sweeteners, the analytical method of ASs as a new tracer for environmental samples requires better sensitivity and selectivity to avoid matrix interference. A highly sensitive method for the simultaneous determination of four frequently-used artificial sweeteners in water samples using solid-phase extraction and ion chromatography triple quadrupole mass spectrometer with an electrospray ionization source ( IC-MS/MS) in negative ion mode was developed. The separation effect of different separation columns was compared and a 2-mm ion chromatography column AS19 was chosen in the experiment. Chromatographic separation of all the 4 artificial sweeteners was carried out in 9 min in isocratic elution mode using 60 mmol/L sodium hydroxide as eluent. Different kinds of solid phase extraction cartridges were evaluated to obtain satisfactory recoveries of all of the analytes. Merk LiChrolut EN (200 mg, 3 mL) was preconditioned with 2 mL of methanol, followed by 2 mL of H2 O. About 200 mL of sample (pH<2. 0) was passed through the cartridge at a flow rate of 4 mL/min, and then the cartridge was eluted using 2 mL of methanol. 2 mm suppresser (75 mA) was used to reduce the background noise and to remove the matrix interference. The limits of detection were below 5. 0 ng/L for various artificial sweeteners based on 3-fold the S/N. The recoveries of different matrices in the samples were 65%-120%. The method described here is time-saving, accurate and precise, and is suitable for monitoring artificial sweeteners in different water matrices. The method has also the potential to trace other contaminants in groundwater.
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ABSTRACT:Objective To explore the differential expression of microRNAs (miRNAs)in steroid-induced osteonecrosis (ON).Methods Bone tissues were selected from patients with steroid-induced ON between the necrotic zone of femoral heads and its femoral neck to analyze the miRNA expression profile using the microarray. The most differentially expressed miR-125a-3p and miR-1 7-5p in microarray analysis were further confirmed by real-time quantitative PCR.Results According to the microarray screening,8 miRNAs were upregulated and 3 miRNAs were downregulated in the necrotic zone of femoral heads samples (Fold>2,P <0.05 ).Results of real-time PCR revealed that miR-125a-3p was upregulated and miR-1 7-5p was downregulated in the necrotic zone of femoral heads samples,which were in agreement with the microarray data.Conclusion MiRNA’s differential expression profile of human steroid-induced ON samples was obtained.Among the differentially expressed miRNAs, miR-125a-3p and miR-1 7-5p are the most apparently differentially expressed miRNAs,which may be related to the pathogenesis and development of steroid-induced ON.
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Objective To investigate the regulatory effects of glucocorticoids on the local renin-angiotensin system in osteoblasts by using MC3T3-E1 cells.Methods Cellular immune histochemistry was carried out to observe the renin-angiotensin system in osteoblasts.And then after 12-hour culture in serum-free solution,MC3T3-E1 cells were divided into four groups:control,dexamethasone (DXM),dexamethasone + mifepristone (DXM+MIF)and mifepristone (MIF).Components of the renin-angiotensin system including angiotensin type 1 receptor (AT1R),angiotensin type 2 receptor (AT2R)and angiotensin converting enzyme (ACE)in osteoblasts were detected at the mRNA and protein levels using Western blot and PCR.The activity of ACE was also measured after 36-hour intervention.Results The results of immunohistochemistry showed that AT1R,AT2R and ACE were all expressed in osteoblasts.The activity of ACE increased obviously after dexamethasone intervention compared with that in the control group,which was blocked by mifepristone.The mRNA levels of AT1R,AT2R and ACE were increased by dexamethasone compared with those in the control group,which was inhibited by mifepristone.The protein levels of AT1R,AT2R and ACE were enhanced by dexamethasone compared with those in the control group, which was blocked when the cells were co-intervened with mifepristone.However,ACE activity and the mRNA and protein levels of AT1R,AT2R and ACE did not change when the cells were intervened with mifepristone alone (P>0.05).Conclusion The local renin-angiotensin system in osteoblasts is activated by dexamethasone through glucocorticoid receptors on osteoblasts,which may be one of the pathogenesis of glucocorticoid-induced osteoporosis.
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Objective To investigate the effect of calcitonin gene-related peptide (CGRP) on angiogenesis of human umbilical vein endothelial cells (HUVECs).Methods The HUVECs were collected from human umbilical core,and the expression of the CGRP receptor-1 was identified though immunofluorescence.After HUVECs were treated with CGRP,the angiogenesis was detected through tube formation experiment.The secretion of vascular endothelial growth factor (VEGF) was detected through ELISA method.The mRNA expression of VEGF,VEGF receptor-1 (FLT1),VEGF receptor-2 (KDR) and CGRP receptor-1 were detected through quantitative PCR (Q-PCR) at 3,7,10 days after culturing.Western blot method was used to detect the protein expression of FLT1 and KDR in HUVECs.Results Immunofluorescence result showed CGRP receptor-1 expressed in HUVECs.CGRP could significantly promote angiogenesis and increase VEGF secretion in direct manner.The Q-PCR results showed that the mRNA expression level of CGRP receptor-1 was significantly higher in CGRP groups than that in control group,especially at 10 days.Compared to the control group,the mRNA and protein expression level of FLT1 and KDR were statistically higher in CGRP groups at different time.Conclusion CGRP can significantly promote angiogenesis of HUVECs in vitro,which may be because it can promote VEGF secretion and expression of FLT1 and KDR in HUVECs.Meanwhile,the increase of CGRP receptor-1 expression also can promote angiogenesis of HUVECs.
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OBJECTIVE: To explore the resource and solution of the expired drugs in citizen family through investigating its constituents and expired time.METHODS: By a random sampling,the records of the reclaimed drugs of a total of 1 000 cases collected from 6 drugstores were analyzed statistically.RESULTS: The main expired drugs reclaimed from citizen family were cardiovascular and cerebral vessels drugs,drugs for respiratory disease,and reinforcing and regulating drugs,and most of which were overdue 50 months ago.CONCLUSIONS: The situation of expired drugs in citizen family is serious,thus measures such as reclaiming expired drugs and popularizing knowledge about taking drugs should be taken to tackle the problem of expired drugs.