Using synthetic peptide to generate to specific antibody against hehatitis B virus protein Pre-S(?) / 中国免疫学杂志

A peptide was synthesized based on the 2l-47 amino acid sequence of Hepatitis B Virus PreSl protein. This peptide (p21-47) was cross-linked to tetanus toxoid and used to immunize rabbit. Rabbit P21-47 not only reacted with the peptide but also reacted with preSl (Large portein of HBV envelope) in Western blotting. Since preSl seems to be involved in attachment of HBV to target cells, this anti P21-47 has the potentiality in studying virus-receptor intereaction as well as for assaying PreS (?)antigen.