The effects of gastric bypass procedures on blood glucose, gastric inhibitory polypeptide and glucagon-like peptide-1 of normal glucose tolerance dogs / 中华外科杂志

<p><b>OBJECTIVE</b>To observe postoperative glucose tolerance, gastric inhibitory polypeptide (GIP) , and glucogan-like peptide-1 (GLP-1) in normal glucose level dogs after undergoing gastric bypass procedures, and to explore the mechanism of gastric bypass procedures to treat type 2 diabetes.</p><p><b>METHODS</b>The 6 dogs with normal glucose tolerance had undergone gastric bypass procedures, and measure preoperative and postoperative oral and intravenous glucose tolerance (at time points 1, 2, and 4 weeks) through changes in blood glucose, insulin, gastric inhibitory polypeptide (GIP), glucagon-like peptide-1 (GLP-1), and measure preoperative and postoperative week 4 pancreatic tissue morphology.</p><p><b>RESULTS</b>Second weeks after operation, the fasting blood sugar was (3.58 ± 0.33) mmol/L, and significantly lower than preoperative (t = 3.571, P < 0.05). The GLP-1 level before oral glucose tolerance test (OGTT) and 30 minutes after OGTT were (0.90 ± 0.21) and (0.91 ± 0.19) pmol/L respectively, and significantly higher than preoperative (t value were -3.660 and -2.971, P < 0.05). GLP-1 levels began to decrease in the second week after surgery. After 4 weeks, the index recovered to the preoperative level. Four weeks after surgery when compared with preoperative, islet morphology, islet number (6.8 ± 0.8 and 7.1 ± 0.8 respectively) and islet cells (16.7 ± 2.5 and 16.3 ± 3.1 respectively) did not change significantly (P > 0.05).</p><p><b>CONCLUSION</b>Gastric bypass procedures could be briefly affect normal glucose tolerance in dogs' blood glucose, insulin and diabetes-related gastrointestinal hormones.</p>

Application of side to side anastomosis between the lesser curvature of stomach and jejunum in laparoscopic Roux-en-Y gastric bypass / 中华胃肠外科杂志

<p><b>OBJECTIVE</b>To evaluate the application of side-to-side anastomosis of the lesser curvature of stomach and jejunum in laparoscopic Roux-en-Y gastric bypass (LRYGB).</p><p><b>METHODS</b>Clinical data of 29 patients with type 2 diabetes mellitus (T2DM) undergoing side to side anastomosis of the lesser curvature of stomach and jejunum in LRYGB from May 2012 to November 2012 in Department of General Surgery, Beijing Tiantan Hospital, Capital Medical University were analyzed retrospectively.</p><p><b>RESULTS</b>All the procedures were successfully completed without conversion to laparotomy. The side-to-side anastomosis of the lesser curvature of stomach and jejunum avoided the laparoscopic suture. No gastrojejunostomy anastomotic bleeding, fistula, obstruction and other complications occurred after operation and no complications of gastrojejunostomy anastomosis were found during a follow up of 1 to 7 months.</p><p><b>CONCLUSIONS</b>Side-to-side anastomosis of the lesser curvature of stomach and jejunum in LRYGB can manipulate the size of anastomosis accurately and avoid the laparoscopic suturing. It is simple and easy to learn.</p>

A preliminary study using RNA interference technique against replication of HBV in vitro / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To identify the siRNA interference ability for the replication of HBV.</p><p><b>METHODS</b>Based on the sequence of HBV in HepG2 2.2.15 cells in GenBank, one sequence targeting the C antigen of HBV was cloned into the RNA polymerase III based expression vector pSuper. This recombinant was electroporated into HepG2 2.2.15 cells and the expression of HBsAg and HBeAg was assayed using ELISA.</p><p><b>RESULTS</b>The construction of the recombinant expression vector pSuper-C and its control vector pSuper was successfully confirmed by the results of enzyme digestion, electrophoresis and sequencing. However, there was no difference between the expression of HBsAg and HBeAg in the supernatant of HepG2 2.2.15 cell culture in the experimental and control groups.</p><p><b>CONCLUSIONS</b>The constructed pSuper-C did not show an interfering effect on the replication of HBV in HepG2 2.2.15 cells. In order to display this effect, further study is needed</p>

Research Advance on Marinomonas / 微生物学通报

In this mini review,some research advance on Marinomonas from domestic and overseas was briefly summarized,mainly including of its classification、ecological distribution、functional genes and bioactive molecules.Furthermore,some suggestion and perspectives for further studies on Marinomonas were also proposed.

Identification of a Marine Bacterium Strain and Characterization of a Novel Functional Gene Cluster Involved in Melanin Biosynthesis / 微生物学通报

A marine bacterium MWYL1,originally isolated from the roots of Spartina anglica growing in a salt marsh near seaside,was identified as a member of the genus of Marinomonas via morphology characterization、physiological test and 16S rDNA sequencing and Blast analysis.The strain was short,rod,gram-negative,grew aerobically and optimally at 28℃.The analysis of 16S rDNA sequence suggests that the sequence similarity values are 97% and 95% with Marinomonas pontica and Marinomonas dokdonensis,respectively.One fosmid clone producing melanin was directly isolated by plating from the genomic library of Marinomonas MWYL1.The novel functional gene cluster involved in melanin biosynthesis was screened after subcloning and sequencing of the 14kb insert in pUC18,further more,the putative functional genes was preminary analyzed using bioinformatics.