ABSTRACT
OBJECTIVE: To investigate the effects of lipid emulsion on acute liver injury induced by trichloroethylene( TCE)in male rats. METHODS: By random number table method,18 specific pathogen free male rats were randomly divided into control group,TCE exposure group and lipid emulsion intervention group,6 rats in each group. The rats of TCE exposure group and lipid emulsion intervention group were given TCE 3 g / kg body weight by intragastric administration,and rats in control group were given the same amount of peanut oil. Rats in lipid emulsion intervention group were given 20% lipid emulsion( 2 mL / kg body weight) by intravenous injection,and the same amount of 0. 9% sodium chloride solution was given by intravenous injection in control group and TCE exposure group. After 24 hours,the activities of alanine aminotransferase( ALT) and aspartate aminotransferase( AST) in serum. Some liver tissues were used for pathological analysis; some for 10% liver homogenate and the levels of malondialdehyde system( MDA),superoxide dismutase( SOD)and glutathione peroxidase( GSH-Px) were detected. RESULTS: Compared with TCE exposure group,edema of liver cell in lipid emulsion intervention group was significantly reduced. Compared with the control group and the lipid emulsion intervention group,the activities of ALT and AST in serum,and the levels of MOD and SOD in liver homogenate were increased( P < 0. 05). The above 4 indexes in the lipid emulsion intervention group were increased compared with the control group( P < 0. 05). There is no significant difference in GSH-Px level in all three groups( P > 0. 05). CONCLUSION: Administration of lipid emulsion can alleviate TCE-induced acute liver damage in rats through improving the lipid peroxidation mechanism.