Surgical approach and orthodontic treatment of mandibular condylar osteochondroma / 대한치과교정학회지

Osteochondroma is a common benign tumor of bones, but it is rare in the mandibular condyle. With its outgrowth it manifests clinically as deviation of the mandible limitation of mouth opening, and facial asymmetry. After the tumor is diagnosed on the basis of clinical symptoms and radiographic examination including cone-beam computed tomography (CBCT) analysis, an appropriate surgery and treatment plan should be formulated. Herein, we present the case of a 44-year-old female patient who visited our dental hospital because her chin point had been deviating to the left side slowly but progressively over the last 3 years and she had difficulty masticating. Based on CBCT, she was diagnosed with skeletal Class III malocclusion accompanied by osteochondroma of the right mandibular condyle. Maxillary occlusal cant with the right side down was observed, but it was confirmed to be an extrusion of the molars associated with dental compensation. Therefore, after intrusion of the right molars with the use of temporary anchorage devices, sagittal split ramus osteotomy was used to remove the tumor and perform orthognathic surgery simultaneously. During 6 months after the surgery, continuous bone resorption and remodeling were observed in the condyle of the affected side, which led to a change in occlusion. During the postoperative orthodontic treatment, intrusive force and buccal torque were applied to the molars on the affected side, and a proper buccal overjet was created. After 18 months, CBCT revealed that the rate of bone absorption was continuously reduced, bone corticalization appeared, and good occlusion and a satisfying facial profile were achieved.

Growth observation and orthodontic treatment of a hemifacial microsomia patient treated with distraction osteogenesis / 대한치과교정학회지

Hemifacial microsomia (HFM) patients may experience emotional withdrawal during their growth period due to their abnormal facial appearance. Distraction osteogenesis at an early age to improve their appearance can encourage these patients. Some abnormalities of the affected side can be overcome by distraction osteogenesis at an early age. However, differences in the growth rate between the affected and unaffected sides during the rest of the growth period are inevitable due to the characteristics of HFM. Therefore, re-evaluation should be performed after completion of growth in order to achieve stable occlusion through either orthognathic surgery or camouflage orthodontic treatment. An eight-year-old patient visited the clinic exhibiting features of HFM with slight mandibular involvement. He received phase I treatment with distraction osteogenesis and a functional appliance. Distraction osteogenesis was performed at the right ramus, which resulted in an open bite at the right posterior dentition. After distraction osteogenesis, a functional appliance and partial fixed appliance were used to achieve extrusion of the affected posterior dentition and settlement of the occlusion adjustment on the unaffected posterior dentition. The patient visited the clinic regularly for follow-up assessments, and at the age of 20 years, he showed facial asymmetry of the mandible, which had deviated to the right side. He received orthodontic treatment to improve the occlusion of his posterior dentition after the growth period. Without orthognathic surgery, stable occlusion and a satisfactory facial appearance were obtained through camouflage orthodontic treatment.

Strategic surgical-combined orthodontic treatment planning of patient with missing incisors on maxilla: a case report

Proper positioning of maxillary incisors is key to success of surgery combined treatment. Establishing surgery plan would be a difficult job if maxillary incisors are lost. Patient who lost all of her maxillary incisors due to accident came for orthodontic treatment. Through careful modification of maxillary archform, pre-surgical orthodontic treatment was conducted with four prosthetic space consolidation. Position of incisors was decided by help of 3D prosthetic set-up, and 1-jaw surgery was planned. After relative short treatment period of 28 months, final prosthesis was done. When alveolar bone loss happens, harmonious prosthesis of upper incisors is difficult. Utilizing mandibular set-back surgery and incisor positioning using 3D set-up could make a better environment for treatment outcome. Strategic pre-surgical orthodontic treatment can allow shorter time and less number of prosthetics.

Orthodontic protraction of the third molars to the posterior teeth missing area

The prolonged neglect of the posterior teeth missing area may cause mesial drift, extrusion, unexpected movement of the adjacent teeth and alveolar bone loss with occlusion collapse. Therefore it is recommended to treat that area by the prosthesis as soon as possible after tooth missing. However, if orthodontic treatment is applied to move the remained teeth, it can create improved biomechanical dentoalveolar environment. The use of the third molars in teeth missing area provides advantages as optimizing of prosthesis size. However, crown shape, location, soundness of the third molar and possible of eruption failure should be considered. In this case report, two patients closed a second teeth missing site and reduced the size of the first and second teeth missing area for an implant by protraction of impacted third molars. This case reports the considerations for closing or reducing the posterior teeth space with protracting the third molars by comparing two patients.

Enhanced compatibility and initial stability of Ti6Al4V alloy orthodontic miniscrews subjected to anodization, cyclic precalcification, and heat treatment / 대한치과교정학회지

OBJECTIVE: To evaluate the bioactivity, and the biomechanical and bone-regenerative properties of Ti6Al4V miniscrews subjected to anodization, cyclic precalcification, and heat treatment (APH treatment) and their potential clinical use. METHODS: The surfaces of Ti6Al4V alloys were modified by APH treatment. Bioactivity was assessed after immersion in simulated body fluid for 3 days. The hydrophilicity and the roughness of APH-treated surfaces were compared with those of untreated (UT) and anodized and heat-treated (AH) samples. For in vivo tests, 32 miniscrews (16 UT and 16 APH) were inserted into 16 Wistar rats, one UT and one APH-treated miniscrew in either tibia. The miniscrews were extracted after 3 and 6 weeks and their osseointegration (n = 8 for each time point and group) was investigated by surface and histological analyses and removal torque measurements. RESULTS: APH treatment formed a dense surface array of nanotubular TiO2 layer covered with a compact apatite-like film. APH-treated samples showed better bioactivity and biocompatibility compared with UT and AH samples. In vivo, APH-treated miniscrews showed higher removal torque and bone-to-implant contact than did UT miniscrews, after both 3 and 6 weeks (p < 0.05). Also, early deposition of densely mineralized bone around APH-treated miniscrews was observed, implying good bonding to the treated surface. CONCLUSIONS: APH treatment enhanced the bioactivity, and the biomechanical and bone regenerative properties of the Ti6Al4V alloy miniscrews. The enhanced initial stability afforded should be valuable in orthodontic applications.

Effect of tribochemical silica coating on the shear bond strength of rebonded monocrystalline ceramic brackets / 대한치과교정학회지

OBJECTIVE: The purpose of this study was to investigate the effect of tribochemical silica coating on the shear bond strength (SBS) of rebonded ceramic brackets using nano-filled flowable composite resin. METHODS: A total of 60 premolars were prepared and divided into 4 equal groups as follows: Tribochemical silica coating (TC) + Transbond XT (XT), TC + Transbond supreme LV (LV), Sandblast treatment (SA) + XT, SA + LV. Treated ceramic brackets were rebonded on the premolars using each adhesive. All samples were tested in shear mode on a universal testing machine. RESULTS: SBS of silica coated groups were high enough for clinical usage (TCLV: 10.82 +/- 1.82 MPa, TCXT: 11.50 +/- 1.72 MPa). But, SBS of the sandblast treated groups had significantly lower values than the tribochemical silica coated groups (SALV, 1.23 +/- 1.16 MPa; SAXT, 1.76 +/- 1.39 MPa; p< 0.05). There was no difference between the shear bond strength by type of adhesive. In the silica coated groups, 77% of the samples showed bonding failure in the adhesive. In the sandblast treated group, all bonding failures occurred at the bracket-adhesive interface. CONCLUSIONS: The result of this study suggest that newly introduced nano-filled flowable composite resin and tribochemical silica coating application on debonded ceramic bracket bases can produce appropriate bond strengths for orthodontic bonding.

Experimental brush wear pattern and cariostatic effect of Biscover / 대한치과교정학회지

OBJECTIVE: The aim of this study was to investigate the experimental brush wear pattern of a light cured surface sealant, Biscover (Bisco, Schaumburg, IL), and to evaluate its cariostatic effect. METHODS: Caries- free human premolars were used for the Biscover coating group (n = 90) and the control group (n = 10). The Biscover coating group was randomly assigned to nine subgroups of 10 each and the control group was assigned to two subgroups of 5 each according to the number of brushing strokes. An experimental 3-body wear test was conducted under different strokes of wear test. Tooth-brushing was accomplished with movement of each brush head set at a frequency of 100 rpm under a force of 1.5 N. Surface roughness was tested before, and after Biscover coating, and after brushing. Then, each of the 10 teeth of both groups were placed in artificial caries inducing solution for 7 days. All tooth surfaces were assessed using scanning electron microscopy. RESULTS: Biscover coated surfaces showed a smoother texture than enamel surfaces. The roughness was increased after experimental brushing and after 10,800 brushing strokes, the whole layer of Biscover wore out. However, teeth in the Biscover coating group had a cariostatic effect in cariogenic conditions. CONCLUSIONS: We suggest that white lesions in orthodontic patients can be suppressed by topical applications of Biscover.

Changes in the Total Antioxidant Ability and Antioxidant Vitamin Levels in the Maternal Venous Plasma of Preeclampsia / 대한산부인과학회지

OBJECTIVE: This study was aimed to compare lipid peroxide level, total peroxyl radical-trapping antioxidative parameter (TRAP) value, and antioxidant vitamin level in the maternal venous plasma between normal pregnancy and preeclampsia. METHODS: Samples of venous plasma were obtained from 38 normal and 24 preeclamptic women. Lipid peroxides levels were measured by thiobarbituric acid reaction. The TRAP values were measured by Wayner's method, although some reaction conditions were modified. Ascorbic acid, retinol, alpah-tocopherol, and gamma-tocopherol were measured by high performance liquid chromatography. RESULTS: There was no significant correlation between the lipid peroxides level in the maternal venous plasma and gestational age in normal pregnancy (n=38, r=0.04, p=NS). The lipid peroxide level in the maternal venous plasma of preeclampsia (n=24) was significantly higher than that of gestational age-matched normal pregnancy (n=26), (4.39 +/- 0.38 vs. 3.23 +/- 0.15 nmol/mg protein, p<0.01). There was no significant correlation between the TRAP value in the maternal venous plasma and gestational age in normal pregnancy (n=38, r=0.02, p=NS). The TRAP value in the maternal venous plasma of preeclampsia (n=24) was significantly lower than that of gestational age-matched normal pregnancy (n=26), (0.33 +/- 0.02 vs. 0.38 +/- 0.02 mM, p<0.05). Ascorbic acid level in the maternal venous plasma of preeclampsia was significantly lower than that of normal pregnancy (377.8 +/- 23.6 vs. 552.2 +/- 52.1 nmol/mL, p<0.05). However, there was no significant difference in maternal venous plasma retinol, alpah-tocopherol, and gamma-tocopherol levels between normal pregnancy and preeclampsia. CONCLUSION: The above results suggest that the imbalance of increased lipid peroxidation and decreased antioxidant activity were in the maternal blood of preeclampsia, and an antioxidant vitamin, ascorbic acid, may be decreased result from counteracting free radical-mediated cell disturbance.

Accuracy of soft tissue profile change prediction in mandibular set-back surgery patients: a comparison of Quick Ceph Image Pro(TM) (ver 3.0) and V-Ceph(TM) (ver 3.5) / 대한치과교정학회지

The purpose of this study was to test and compare the accuracy and reliability of soft tissue profile predictions generated from two computer software programs (Quick Ceph Image Pro(TM) (ver 3.0) and V-Ceph(TM) (ver 3.5)) for mandibular set-back surgery. The presurgical and postsurgical lateral cephalograms of 40 patients (20 males and 20 females) were traced on the same acetate paper with the reference taken as the cranial base outline. The presurgical skeletal outlines were digitized onto each computer program and the mandible was moved to mimic the expected surgical procedure with reference to the mandibular anterior border and lower incisor position of the actual postsurgical skeletal outline. The soft tissue profile was generated and the amount and direction of skeletal movement was calculated with each software. The predicted soft tissue profile was compared to the actual postsurgical soft tissue profile. There were differences between the actual and the predicted surgical soft tissue profile changes in the magnitude and direction, especially the upper lip, lower lip and the soft tissue chin (p < 0.05). Quick Ceph had more horizontal measurement errors and thickness errors for the upper lip and lower lip, but V-Ceph had more vertical measurement errors of the lower lip (p < 0.05). There was a positive correlation between the prediction errors and the amount of mandibular movements in the vertical position of Sn, the horizontal position of Ls and the upper lip thickness for V-Ceph, and there was a negative correlation in the horizontal position and the thickness of the lower lip for Quick Ceph (p < 0.05). However, all of the prediction errors of both imaging softwares were ranged within 3 mm, and this was considered to be allowable clinically.

The effect of contamination on bonding of orthodontic brackets with a self-etching primer/adhesive / 대한치과교정학회지

The purpose of this study was to investigate the influence of water, saliva, and blood contamination on the bonding strenght of metal brackets with a self-etching primer/adhesive to enamel. Ninety-six extracted human teeth were divided into four groups. The brackets were bonded to enamel with a self- etching primer (3M/Unitek Dental Products, Monorovia, California) according to one of four protocols. The teeth were bonded in a dry condition (group D) or in contamination with distilled water (group W), artificial saliva (group S), or fresh human blood (group B). Shear bond strengths were tested using an Instron Universal testing machine. After debonding, bracket and tooth surfaces were examined with a stereomicroscope. In each group, four samples were selected and examined with a Scanning electron microscope of the prepared enamel surface and resin-enamel interface. The results obtained were summarized as follows: Shear bond strength in group D (15.22 +/- 2.86 MPa) and W (16.20 +/- 3.85 MPa) were higher than in group B (12.56 +/- 2.94 MPa) (p 0.05). There was a tendency to have less residual adhesive remaining on the enamel surfaces of group B than group D. The SEM morphology of group D and W showed a more roughened etching pattern than group S and B. Water or saliva contamination on bonding of orthodontic brackets with Transbond plus self etching primer had almost no influence on bond strength. In this study, the blood contaminated group showed the lowest bond strength, but it was above the clinically acceptable bond strength (5.9-7.8 MPa, Reynold, 1975). The results of this study suggest that acceptable clinical bond strengths can be obtained in wet conditions when self-etching adhesives are used.

Effects of light direction and exposure times of plasma arc light on shear bond strength of metal brackets / 대한치과교정학회지

The purpose of this study was to compare the effects of different light direction, exposure times and setting times when using plasma arc light on shear bond strength of metal brackets. 240 extracted human premolars were randomly assigned to one of 16 groups. Standardized brackets were bonded to enamel using different light curing units (Plasma arc light and Halogen light), exposure times (Plasma arc light 2, 4, 6 seconds and Halogen light 20 seconds), and light directions (Vertical direction [V] and Oblique direction [O]). 8 groups were tested after 5 minutes and the remaining 8 groups after 24 hours. The metal brackets were bonded with Transbond XT. Shear bond strength was measured by a universal testing machine. The results were as follows: There were no differences between the shear bond strengths of the Vertical groups (V) and Oblique groups (O), regardless of exposure times and types of light curing units (p > 0.05). The shear bond strength of the group with 2 seconds of plasma light were significantly lower than other exposure time groups (p < 0.05). The shear bond strength tested after 5 minutes was lower than after 24 hours (p < 0.05). The Adhesive Remment Index (ARI) score showed no statistically significant difference among the different groups. The results of this study suggested that the light direction of plasma arc light had no influence on the shear bond strength of metal brackets to enamel, and exposure times more than 4 seconds produced shear bond strengths similar to those produced with a conventional halogen curing light.

A Case Diagnosed to Cushing's Syndrome Caused by Adrenal Cortical Adenoma during Pregnancy after Ovulation Induction / 대한산부인과학회잡지

This woman was a secondary infertile patient with amenorrhea, hirsutism, and hyperprolactinemia due to excessive secretion of androgen. We had an impression that her disease was polycystic ovary syndrome, and she became pregnant after ovulation induction. Complications arised, such as hypertension, diabetes and pulmonary edema at 23rd week of pregnancy and physical findings of Cushing's syndrome were observed. Cushing's syndrome caused by adrenal cortical adenoma was diagnosed through endocrine and radiologic examination. We let her keep pregnancy through adjusting hypercorticolism with metyrapone to restrain abnormal steroidogenesis during pregnancy. After delivery, we carried out adrenalectomy through laparoscopic surgery and then can't find out any problem to be appeared to the mother and her neonate. We report this case with brief discussion.

Comparison of bracket bond strength in various directions of force / 대한치과교정학회지

The purpose of this study was to evaluate the bond strength of orthodontic brackets bonded to metal bar with chemically cured adhesive (Ortho-one, Bisco Co, USA) in various types and directions of force application. Three types of metal bracket with different bracket base configurations; Micro-Loc base(Tomy Co, Japan), Chessboard base(Daesung Co, Korea), Non-etched Foil-Mesh base(Dentaurum, Germany); were used in this study. Peel, shear, tensile bond strengths were measured by universal testing machine and compared each other. The peel force directions applied were 0degrees, 15degrees, 30degrees, 45degrees, 60degrees, 75degrees, 90degrees. And then, in consideration of the different surface area of the bracket bases, the bond strength per unit area were calculated and compared. The results obtained were summarized as follows : 1. The bond strengths according to the types and the directions of the forces were greatest at the shear forces in all three bracket base configuration groups(p < 0.01). 2. As the peel force direction grew higher in degree, peel bond strength decreased. The patterns of peel bond strength change according to force direction was similar in all three bracket base configurations. The minimum bond strength was 60 degree-peel bond strengths in all three bracket base configurations. 3. In Micro-Loc base group, minimum peel bond strength(60PBS) was in 29% level of shear bond strength and 52% level of tensile bond strength. In Chessboard base group, 60PBS was in 34% level of shear bond strength and 61% level of tensile bond strength. In Non-etched Foil-Mesh base group, 60PBS was in 34% level of shear bond strength and 55% level of tensile bond strength. 4. The bond strengths per unit area were lowest in Non-etched Foil-Mesh base group and highest in Chessboard base group(p < 0.05). However, there were no differences in shear bond strength, tensile bond strength, 75PBS and 90PBS per unit area between Micro-Loc and Chessboard base groups.

Airway size in malocclusions with hyperdivergent skeletal pattern / 대한치과교정학회지

The pharynx have significant locations and functions because it forms a part of the unit in which respiration and deglutition are carried out. Because of the closed relationship between the pharynx and the dentofacial and craniofacial structure, many studies are carried out on this subjects. The purpose of this study were to compare the airway size including pharynx, soft palate, tongue and hyoid bone between normal and hyperdivergent skeletal pattern and to evaluate the change of those size in different age. The sample of this study were consisted of 51 subjects in normal group, 52 subjects in hyperdivergent group. Each was divided into two subgroups by age ; child group(9-12yr old) and adult group(18yr old over). The lateral cephalometric radiographs were taken and the distance, angle and ratio of the facial skeleton, pharynx, soft palate, tongue and hyoid bone were measured and evaluated statistically. The result obtained were summarized as follows : 1. SN-Mn angle, FMA, and Pal-Mn angle were significantly larger in child group than adult group, but the lower anterior facial height(LAFH) and facial height ratio(FHR) were significantly smaller in child group than adult group. Occlu-Mn angle was significantly steep in hyperdivergent group than normal, but not significantly different between child group and adult group. 2. Hyperdivergent group and child group had the stronger correlation between SN-Mn angle, FMA, Pal-Mn angle, LAFH and FHR and airway size than normal group and adult group. Hyperdivergent child group had significant negative correlation between SN-Mn angle, FMA, Pal-Mn angle and sagittal skeletal dimension of nasopharynx and sagittal depth of nasopharyngeal lumen. Significant positive correlation were seen between LAFH and vertical measurement of airway. 3. There was no difference of the linear measurement of airway size between hyperdivergent group and normal group. 4. Adult group had smaller posterior pharyngeal wall, larger nasopharyngeal depth longer nasopharyngeal height and longer pharyngeal length than child group. 5. The sagittal measurement of hyoid bone had no difference between child group and adult group. But adult group had larger vertical measurement of hyoid bone than child group.

Shear bond strength of metal orthodontic brackets bonded with Self-Etching Primer / 대한치과교정학회지

The purpose of this study was to evaluate the effects of a self-etching primer on the shear bond strength of orthodontic brackets and on the failure pattern of bracket-adhesive interfaces in dry or wet condition. Brackets were bonded to extracted human teeth according to one of three experimental protocols. In the Group P, teeth were etched with 37% phosphoric acid. After the Transbond XT Primer was applied onto the etched surfaces, the brackets were bonded with Transbond XT(3M, Unitek, Monrovia, Calif) and light cured for 40 seconds. In the Group SD, a self-etching primer(3M, Unitek, Monrovia, Calif) was placed on the enamel for 3 seconds and gently evaporated with air, as suggested by the manufacturer. The brackets were then bonded with Transbond XT as in the Group P. In the Group SW, artificial saliva was applied to the enamel surface for 10 seconds to allow complete hydration of the surface before application self-etching primer. The brackets were then bonded following the procedures of Group SD. Each group was divided into 2 sub-groups(0.5h, 24h) according to debonding time. Shear bond strengths were measured by Instron universal testing machine. After debonding, the teeth and brackets were examined under scanning electron microscope and assessed with the adhesive remnant index. The result obtained were summarized as follows ; 1. The shear bond strengths were high enough to use clinically in all testing groups, but the shear bond strengths of Group SD and SW were significantly lower than Group P(p 0.05). 4. There was a greater frequency of ARI score of 0 and 1 with the Group P. On the other hand, there was a higher frequency of ARI scores of 2 and 3 with Group SD and Group SW(p < 0.05).

Late Postpartum Eclampsia: A Case Report / 대한주산의학회잡지

Although the technique of antenatal care is developed, the incidence of late postpartum eclampsia has been increased and its prevention is difficult. In addition, the diagnosis is more difficult if the patient did not show any symptoms of preeclampsia before delivery. We described one case of late postpartum eclampsia that occurred on 7th day after preterm delivery and has not been diagnosed before delivery including a review of this literature.

Dentoalveolar Characteristics according to facial types of Class III Malocclusion / 대한치과교정학회지

This study was performed to compare the pattern of dentoalveolar characteristics in different vertical and anteroposterior skeletal types in skeletal Class III malocclusion. The samples selected for this study were consisted of 60 subjects(29 males and 31 females, mean age; 19.7 years) in Class III group, 43 subjects(14males and 29 females, mean age: 20.5 years) in normal group. The findings of this study were as follows; 1. The IMPA of the Class III group was smaller than that of the Class I group(p<0.01). 2. In the Class III groups, SNB and NtoPog had negative correlation with IMPA(p<0.01). The SNB and NtoPog had correlations with SNU1, FHU1 and PalU1(p<0.01) in the male samples, and in the female samples, the SNB and NtoPog had correlations with SNU1(p<0.01). 3. In the Class III male samples, SNMP, FMA, PalMP had negative correlation with IMPA(p<0.01). SNMP, FMA, PalMP had not significant correlation with SNU1, FHU1, PalU1. In the Class III female samples, FMA, PalMP had negative correlation with IMPA(p<0.01). 4. In the high angle group of Class III samples, SNU1, IMPA is smaller than that of low angle group of Class III samples(p<0.05).

A Case of Identification of Marker Chromosome by comparative genomic hybridization and fluorescence in situ hybridization / 대한임상병리학회지

Comparative genomic hybridization (CGH) has been used to identify deletions and amplifications, particularly in neoplastic samples. CGH provides a new possibility searching genomes for imbalances of genetic material. We described the combined use of CGH and fluorescence in situ hybridization (FISH) to identify the origin of a marker chromosome in a child with mental retardation. Giemsa banding of metaphases from cultured lymphocytes showed a marker chromosome. The Karyotype was 47,XX,+mar. CGH revealed that the additional material originated from 15q. FISH confirmed this finding with whole chromosome paint for chromosome 15 and with a D15S10 (15q11-13) probe. This case demonstrates the efficient use of CGH and confirmatory FISH for the identification of chromosomal material of unknown origin.

Effect of estrogen on growth hormone receptor expression of human periodontal ligament cell line / 대한치과교정학회지

The present studies were performed to investigate the interaction of 17beta-estradiol and human growth hormone(hGH) on the proliferation of human periodontal ligament(hPDL) cell. The independent effects of 17beta-estradiol and hGH on hPDL cell proliferation were investigated and the effects of hGH on hPDL cell proliferation after 17beta-estradiol pre-treatment were also investigated. Lastly, the change of hGH receptor expression in hPDL cell after 17beta-estradiol pre-treatment were investigated The obtained results were as follows; 1. The treatment of 17beta-estradiol or hGH had no significant effects on hPDL cell proliferation. 2. After pre-treatment of 17beta-estradiol, hGH stimulated the proliferation of the hPDL cell, regardless of hHG concentration. 3. Although there was not hGH receptor in the hPDL cell, hGH receptors were expressed in hPDL cell after more than 6 hours pre-treatment cf 17beta-estradiol. 4. The effect of hGH on hPDL, cell proliferation was related to the hGH receptor expression. 17beta-estradiol pre-treatment contributed to the hGH effects on the hPDL cell by stimulating hGHR expression.

Detailed Characterization of Chromosomal Breakpoints in an ALL, L3 using Cross Species Color Banding / 대한임상병리학회지

Karyotype analysis by G-anding is the standard method for identifying numerical and structural chromosomal aberrations in cytogenetic laboratories. However, the origins of marker chromosomes, subtle translocations, or complex chromosomal rearrangements are often difficult to identify with certainty. Cross-pecies color banding is a new FISH-ased screening technique that enables the generation of a specific color banding pattern for each human chromosome based on the genomic homologies between man and various species of apes. We report application of Cross-pecies color banding (RxFISH) to characterize the chromosomal rearrangements of one leukemia sample the G-and karyotype of which were incomplete. The combination of G-anding and RxFISH in this case study yielded additional information beyond that obtained by either technique used alone in determining the precise breakpoints in complex chromosomal rearrangements.