Detection of Mitochondrial ATP-Sensitive Potassium Channels in Rat Cardiomyocytes

Mitochondrial ATP-sensitive potassium (mitoKATP) channels play a role in early and late ischemic preconditioning. Nevertheless, the subunit composition of mitoKATP channels remains unclear. In this study, we investigated the subunit composition of mitoKATP channels in mitochondria isolated from rat cardiac myocytes. Mitochondria were visualized using the red fluorescence probe, Mitrotracker Red, while mitoKATP channels were visualized using the green fluorescence probe, glibenclamide-BODIPY. The immunofluorescence confocal microscopy revealed the presence of Kir6.1, Kir6.2 and SUR2 present in the cardiac mitochondria. Western blot analysis was carried to further investigate the nature of mitoKATP channels. For SUR proteins, a 140-kDa immunoreactive band that corresponded to SUR2, but no SUR1 was detected. For Kir6.2, three bands (~4, ~6, and ~0 kDa) were detected, and a specific ~6-kDa immunoreactive band corresponding to Kir6.1 was also observed. These observations suggest that the subunits of mitoKATP channels in rat myocytes include Kir6.1, Kir6.2, and a SUR2-related sulfonylurea-binding protein.

Identification of a Marker Protein for Cardiac Ischemia and Reperfusion Injury by Two-Dimensional Gel Electrophoresis and Matrix-Assisted Laser Desorption Ionization Mass Spectrometry

The purpose of the present study was to evaluate the expression of cardiac marker protein in rabbit cardiac tissue that was exposed to ischemic preconditioning (IPC), or ischemiareperfusion injury (IR) using two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). We compared 2DE gels of control (uninjured) cardiac tissue with those of IPC and IR cardiac tissue. Expression of one protein was detected in IR heart tissue, however the protein was not detected in the samples of control and IPC tissue. To further characterize the detected protein molecule, the protein in the 2D gel was isolated and subjected to trypsin digestion, followed by MALDI-MS. The protein was identified as myoglobin, which was confirmed also by Western blot analysis. These results are consistent with previous studies of cardiac markers in ischemic hearts, indicating myoglobin as a suitable marker of myocardial injury. In addition, the present use of multiple techniques indicates that proteomic analysis is an appropriate means to identify cardiac markers in studies of IPC and IR.

Wearing Sensibility Related to Body Shape in Middle Aged Women / 체질인류학회지

The present study was performed to examine wearing sensibility related to body shape in middle aged women. Body measurements, front and side views and index values between girth measurements were used as the body shape comparison elements. The results obtained are as follows. 1. Bust girth, waist girth and hip girth in middle aged women showed greater values (respectively : 6%, 11.4% and 3.4%) than in young aged women. 2. For the drop value between hip girth and bust girth, the results were 7.3 cm in tweenties, 3.6 cm in thirties, 4.2 cm in forties, 1.5 cm in fifties and 0.7 cm in sixties. 3. Front and side shape examination: Assuming a value of 1.00 for the waist breadth and waist depth, the results in middle aged women were 0.7 and 1.2 in nipple and hip breadth, and 1.1 and 1.1 in bust and hip depth. 4. The sensibility related to body shape, abdoman, waist and arm were most reshaped elements by aging.

Changes of Somatotypes and Body Proportions in Korean Youths / 체질인류학회지

The aim of the present study is to try to quantify the changes in somatotypes and to determine, if any, the distinctive features of young Koreans while they are growing. It is based on the data offered by the Korean National survey of 1997 and the subjects taken in account were those ranging from 6 to 17 years of age. The analized contents used by us here were the annual growth rates, the body shape grouping by canonical analysis statistical method and body proportion rate changes. 1. The result for the growth rates showed that youngsters have their peak growth velocity between 12 and 14 years of age in case of boys and between 8 and 10 in case of girls. 2. The result for the body shape grouping shows that, the body type of the young childhood (from 6 to 9 years of age), the body type of the puberty (from 10 to 12 years of age) and the body type of the junior adolescent (from 13 to 15 years of age), thus, leads us to distinguish 3 groups. 3. As examples of items body proportion rates, we found for instance that the ratio of the span for the height was 0.996H in case of grown males Korean between 18~24 years of age, 0.994H for ages from 25 to 50, and in case of grown females 0.984H for ages 18~24 and 0.985H for ages 25~50, showing then a slightly longer ratio for males. There was no noticable differences though betwen the two big age groups (18~24 years of age and 25~50 years of age). 4. Body proportion rates showed also that the grown-up body features were attained in average both for boys and girls, around the age of 13 years.