ABSTRACT
Objective To investigate the expressions of acid sensing ion channel 1a(ASIC1a) and activator protein-1 (AP-1) in hepatocellular carcinoma,and to analyze the relationship between expressions and clinical features.Methods At the transcription level,expression spectroscopy chips and bioinformatics were used to analyze the changes of signal pathways before and after ASICla interference in hepatocellular carcinoma cells.63 cases of HCC and 42 cases of tumor adjacent tissue samples were chosen from the Third People's Hospital of Wuxi,between January 2010 and December 2014.Immunohistochemical staining was utilized to detect the expressions of ASIC1 a,c-Jun,c-Fos.The relationships among the three were analyzed by the nonparametric Spearman rank correlation coefficient.Results After ASIC1a inhibition,the expression of AP-1 (c-Jun and c-Fox) decreased significantly.The expressions of c-Jun and c-Fox were greatly decreased in interference group compared with control group.The positive rates of ASIC1a,c-Jun and c-Fos in HCC tissues were significantly higher than that of the adjacent tissues,68.3％ vs.19.0％,55.6％ vs.11.9％,47.6％ vs.11.7％ (P ＜ 0.05).Correlation analysis showed that the expression of ASIC1a was positively correlated with c-Jun and c-Fos expression (r =0.404,0.309,P ＜ 0.05).The expressions of ASIC 1 a,c-Jun and c-Fos were not related to age,tumor diameter and gender (P ＞ 0.05),which were related to the clinical stage,AFP and lymph node metastasis of the tumor (P ＜ 0.05).Conclusion ASIC1a may.affect the development of hepatocellular carcinoma through the downstream gene AP-1.
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The preparation of electrophoretic microcolumn and its application to the electrophoretic separation of amino acids with a 2-mm I.D. fused-silica microcolumn packed with uniform quartz microncrystal prepared by hydrothermal synthesis are reported. With 1.5 mmol/L disodium phosphate buffer solution (pH 11.5) containing 30% (V/V) methanol, the tryptophan, phenylalanine and tyrosine were separated by the microcolumn electrophoresis and detected by an UV spectrophotometer without derivatization. The limits of detection were 0.038, 0.21 and 0.20 mol/L, respectively. The separation efficiency of tryptophan was 4.4×104 plates/m. The sample capacity of the electrophoretic microcolumn achieved 35 μL. The precisions of the microcolumn electrophoresis were satisfactory. The thermal effects of the electrophoretic microcolumn that without packing, packed with 360 μm quartz sand and with 9 μm length quartz microncrystal were discussed, respectively. It was found that the electrophoretic microcolumn packed with quartz microncrystal was able to inhibit Joule heat, increase sample capacity and enhance detection sensitivity. The microcolumn electrophoresis is one of the high-performance separation techniques for an in-situ, real-time and portable electrokinetic flow analysis system.
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Objective To study the effect of HAI and/or PVI after radical resection of hepatocellular carcinoma (HCC) for preventing recurrence.Methods From Jun. 1995 to Apr. 2000, 49 patients with HCC were undergone radical tumor resection. They were divided into 4 group: the treatment combined with HAI and PVI was given in group I(12 cases), HAV alone in group II (8 cases); PVI alone in group III (15 cases); nothing in group IV (14 cases). All patients were followed up for 6 months to 5 years. Results Recurrence rate at one year didn't show significantly lower in group I, II and III than that in grou IV. Survival rate in one year showed no significant difference in 4 groups. Recurrence rate in three years was significantly lower in group I and II than that in group IV. Survival rate in three years was significantly higher in group I and II than that in group IV. Recurrence rate and survival rate in five years showed no significante difference in four groups. Conclusions The treatment of radical resection of HCC combined with HAI and/or PVI is simple and safe. It is an useful method for preventing tumor recurrence.
ABSTRACT
Objective To study the effect of HAI and/or PVI after radical resection of hepatocellular carcinoma (HCC) for preventing recurrence.Methods From Jun. 1995 to Apr. 2000, 49 patients with HCC were undergone radical tumor resection. They were divided into 4 group: the treatment combined with HAI and PVI was given in group I(12 cases), HAV alone in group II (8 cases); PVI alone in group III (15 cases); nothing in group IV (14 cases). All patients were followed up for 6 months to 5 years. Results Recurrence rate at one year didn't show significantly lower in group I, II and III than that in grou IV. Survival rate in one year showed no significant difference in 4 groups. Recurrence rate in three years was significantly lower in group I and II than that in group IV. Survival rate in three years was significantly higher in group I and II than that in group IV. Recurrence rate and survival rate in five years showed no significante difference in four groups. Conclusions The treatment of radical resection of HCC combined with HAI and/or PVI is simple and safe. It is an useful method for preventing tumor recurrence.