ABSTRACT
Members of the bZIP transcription factor family play crucial roles in the regulation of plant development, biosynthesis of secondary metabolites, and response to abiotic and biotic stresses. To date, multiple bZIPs have been identified and investigated in numerous plant species. However, few studies have characterized bZIPs from Dimocarpus longan Lour. In this study, nine bZIPs from D. longan were identified from RNA-Seq data and further verified using the NCBI conserved domain search tool and Pfam database. Bioinformatics tools were used to systematically analyse the physicochemical properties, protein structures, multiple sequence alignment, motif compositions, evolutionary relationships, secondary structures, subcellular localization, phosphorylation sites, signal peptides, GO annotations and protein–protein interactions of the DlbZIPs. The expression patterns of the nine DlbZIPs were evaluated by qRT-PCR in roots and leaves and in response to varying durations of a 38C heat treatment. DlbZIP3, DlbZIP5, DlbZIP6 and DlbZIP7 were differentially expressed between root and leaf tissues. All nine DlbZIPs responded to heat treatment in both roots and leaves, but their specific expression levels differed. DlbZIP4 and DlbZIP8 were highly expressed in roots after heat treatment, whereas DlbZIP1 and DlbZIP5 were highly expressed in leaves after heat treatment. These findings lay a foundation for increasing active secondary metabolite content and improving abiotic stress tolerance in D. longan using transgenic technology
ABSTRACT
Objective @#To investigate the effect of orthodontic and orthognathic treatment on patients with skeletal Class Ⅱ malocclusionby studying the changes of soft and hard tissues in maxillofacial region, to analyze the correlation between soft and hard tissues in patients with skeletal classⅡ in order to provide reference and guidance for combination therapy.@*Methods @#23 patients with skeletal classⅡmalocclusion treated by combined orthodontic and surgical treatment were selected, 21 parameters that can reflect the soft and hard tissue and the facial appearance were recorded in the imaging data before and after orthodontic-orthognathic treatment, The correlation between the soft and hard tissue of the patients before treatment was analyzed.@*Results @#There were significant differences between the before and after orthodontic-orthgnathic treatment in the 19 of above mentioned parameters, except for ANS-Me/N-Me(%) and Pg-Pg′ (mm). By analyzing the correlation between the hard tissue index and the soft tissue profile of the patients before treatment, it can be found that the facial aesthetics is influenced by many hard tissue parameters.@*Conclusion @# The effect of orthodontic and orthognathic technique in the treatment of skeletal ClassⅡ malocclusion is considerable. Hard tissue profile of patients with skeletal ClassⅡ malocclusion forms the basis and support of soft tissue profile. At the same time, soft tissue has some independence. However, the exact quantitative relationship remains to be further studied.