Clinical significance and anatomical relationship between the inferior margin of oval window and the endosteum of basal cochlear turn / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To investigate the safety range of drilling and fenestration on promontory inferior to the oval window in difficult stapedectomy via anatomical study of the relationship between the inferior margin of oval window and the endosteum of basal cochlear turn.</p><p><b>METHODS</b>By means of histological sections, the distances between the inferior margin of oval window and different locations of endosteum of basal cochlear turn, the corresponding height of the projecting endosteum and the thickness of promontory bone, on three vertical sections through the anterior, posterior and midpoint of the inferior edge of oval window were measured respectively.</p><p><b>RESULTS</b>The promontory bone inferior to the posterior point of the oval window was thickest, with an average thickness of about 1.1 mm. The endosteum of basal turn at this portion was lowest, with an average height of 0.2 mm. Furthermore, the shortest distance here between the inferior edge of oval window and the endosteum could be no more than 0.3 mm.</p><p><b>CONCLUSION</b>Relatively safe location of drilling on promontory should be selected on promontory inferior to the posterior point of the oval window, with the direction of drilling outward and downward.</p>

Computer simulation of projectile injuries to pig mandibular angle / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To investigate the feasibility of computer simulation in maxillofacial firearm injury.</p><p><b>METHODS</b>The three-dimensional finite element models and simulations of 7.62 mm, 5.56 mm standard bullets projectile injuries to pig mandibular angle were established by using MIMICS, ANSA, LS-DYNA and LS-POST software. Based on the simulation results, the bullet hole diameters, energy loss values, energy loss rates, von Mises stress, effective strain, effective strain rate dynamic contours at different time points were used for biomechanical analysis.</p><p><b>RESULTS</b>The damage processe of 7.62 mm, 5.56 mm standard bullets projectile injury to pig mandibular angle were simulated successfully. The injury rate of 7.62 mm standard bullet and injury severity of the mandible were higher than that of 5.56 mm standard bullet.</p><p><b>CONCLUSIONS</b>Computer simulation can simulate maxillofacial firearm injuries effectively and may become an important method for oral and maxillofacial firearm injuries analysis.</p>

Differential expression of toll-like receptors in chronic suppurative otitis media and cholesteatoma / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To investigate the differential expression of toll-like receptor 2 (TLR2), toll-like receptor 4 (TLR4) and their potential role in the pathogenesis of chronic suppurative otitis media and cholesteatoma.</p><p><b>METHODS</b>Normal canal skin of 30 patients with tympanosclerosis were enrolled as control, 30 cases with chronic suppurative otitis media and 30 patients with cholesteatoma were studied. Real-time PCR, Western blot and Immunohistochemistry were preformed to detect the expression of TLR2/TLR4 in normal canal skin, mucosa and granulation tissue of chronic suppurative otitis media, mucosa, granulation tissue, cholesteatoma epithelium of cholesteatoma, and the differential expression were analyzed.</p><p><b>RESULTS</b>(1) the mRNA and protein expression of TLR2 and TLR4 were detected in all normal canal skin, mucosa and granulation tissue of chronic suppurative otitis media, mucosa, granulation tissue, cholesteatoma epithelium of cholesteatoma. (2) Both mRNA and protein level of TLR2/TLR4 in mucosa of chronic suppurative otitis media and cholesteatoma were higher than those in normal canal skin, but lower in cholesteatoma epithelium, there was no significant difference in mucosa of the two otitis media groups. (3) The mRNA and protein expression of TLR2/TLR4 in granulation tissue of chronic suppurative otitis media and cholesteatoma were significant increased when compared with normal canal skin, and TLR2 expression level was higher in granulation tissue of cholesteatoma than in chronic suppurative otitis media. (4) TLR2/TLR4 positive cells mainly infiltrated in granulations, significantly more than in normal skin, while fewer in the epithelium of cholesteatoma.</p><p><b>CONCLUSIONS</b>Differential expression of TLR2 and TLR4 in mucosa suggests middle ear is a TLR2/TLR4 participated functional modulation of the innate immune system and also suggests that they may play a different role in the pathophysiology of chronic otitis media and cholesteatoma.</p>

Clinical significance of intact canal wall mastoidectomy combined with facial recess opening in the treatment of secretory otitis media of children / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To investigate the clinical significance of intact canal wall mastoidectomy combined with facial recess opening in the treatment of secretory otitis media of children.</p><p><b>METHODS</b>The clinical data of 17 children (19 ears) with recurrent secretory otitis media yet failed tube insertion more than 3 times, and treated with intact canal wall mastoidectomy combined with facial recess opening, in the Department of Otorhinolaryngology Head and Neck Surgery, Second Affiliated Hospital of SUN Yat-sen University, were reviewed. And because of the eustachian tube dysfunction, 7 ears simultaneously accepted tube insertion, which were removed after 1 to 3 months.</p><p><b>RESULTS</b>Pathological examination of the lesions in middle ear and mastoid of the 19 ears, revealed cholesterol granuloma in 9 ears and inflammatory granulation in 10 ears. All 19 ears recovered with normal tympanic membranes. There were 16 ears with type A tympanogram and 3 ears with type C tympanogram (negative pressure less than 150 mm H₂O). The air-bone gaps were less than 15 dB in 3 months after surgery. There was no recurrence in all cases after 2 - 3 years follow-up.</p><p><b>CONCLUSIONS</b>In case of recurrent otitis media in children, especially when tube insertion is ineffective, intact canal wall mastoidectomy combined with facial recess opening can be adopted to clear the lesions thoroughly, and to establish long-time and effective ventilation of eustachian tube, tympanic cavity, tympanic antrum, and mastoid.</p>