ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of resveratrol on the apoptosis of pancreatic acinar cells in rats with severe acute pancreatitis (SAP) and explore the mechanism of such effect.</p><p><b>METHOD</b>SD rats with 3.5% sodium taurocholate-induced SAP were treated with resveratrol, and the serum amylase was detected with automatic biochemistry analyzer. The apoptosis of the pancreatic acinar cells in the rats was detected by TUNEL assay, and the expression of Fas and FasL genes was determined by RT-PCR and Western blotting. The pathological changes of the pancreas were observed under optical microscope.</p><p><b>RESULTS</b>Compared with SAP group, the resveratrol-treated rats showed obviously decreased serum amylase and scores for pancreatic histopathological lesions. Resveratrol treatment significantly increased the apoptotic indices of pancreatic acinar cells and the levels of FasL mRNA and protein in rats with SAP.</p><p><b>CONCLUSION</b>Resveratrol produces important therapeutic effect on SAP in rats by inducing pancreatic acinar cell apoptosis possibly as a result of up-regulated FasL gene expression.</p>
Subject(s)
Animals , Male , Rats , Apoptosis , Fas Ligand Protein , Genetics , Metabolism , Pancreas, Exocrine , Pathology , Pancreatitis, Acute Necrotizing , Drug Therapy , Pathology , RNA, Messenger , Genetics , Metabolism , Random Allocation , Rats, Sprague-Dawley , Stilbenes , Therapeutic Uses , Taurocholic Acid , Up-RegulationABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of survivin in acute pancreatitis in rats.</p><p><b>METHODS</b>Acute pancreatitis was induced in rats by retrograde injection of sodium taurocholate into the pancreaticobiliary duct. The expressions of survivin in the pancreatic tissues was detected by immunohistochemisty, Western blotting and RT-PCR, and the apoptotic ratio of the acinar cells was determined by TUNEL assay.</p><p><b>RESULTS</b>Survivin was not detected in the control group, and in rat models of acute pancreatitis, the expressions of survivin protein and mRNA increased but the apoptotic index of the acinar cells decreased gradually with the severity of inflammation.</p><p><b>CONCLUSION</b>Survivin is involved in the regulation of acinar cell apoptosis and also the necrosis of the apoptotic acinar cells through its anti-apoptosis activity to aggravate acute pancreatitis, suggesting its value as a promising marker in predicting the severity of acute pancreatitis.</p>