Quantitative analysis of calycosin-7-O-β-D-glucoside and formononetin in Astraglus membranaceus var. mongholicus by different processing methods / 中草药

Objective: An HPLC method was established to determine the content of calycosin-7-O-β-D-glucoside and formononetin in Astraglus membranaceus var. mongholicus. To study the influence of habitats, processing methods, and planting ages on the quality of A. membranaceus var. mongholicus. Methods: The HPLC condition was as follows: column: Dikma C18 (250 mm×4.6 mm, 5 μm); mobile phase: Acetonitrile-0.2% formic acid (gradient elution); flow speed: 1.0 mL/min; detection wavelength: 260 nm; temperature of column: 30℃. Results: Comparing the content of calycosin-7-O-β-D-glucoside and formononetin in 40 samples of A. membranaceus var. mongholicus from 11 different habitats, we could get the following: The processing methods of A. membranaceus var. mongholicus were cleaning and natural drying; Bayinnaoer city Wu Qianqi ming'an town head part was the best habitat for A. membranaceus var. mongholicus. The quality of A. membranaceus var. mongholicus cultivars grown for two years and three years had no difference. The quality of wild species was superior cultivars. Conclusion: The method is simple and rapid for determination, the contents of calycosin-7-O-β-D-glucoside and formononetin in A. membranaceus var. mongholicus. It can be used as a scientific and reliable basis for formulating the reasonable standard of cultivating and processing methods of A. membranaceus var. mongholicus.

Expression of pungent-taste herbs and their applications in clinical compatibility / 中草药

In this article, authors summarized the connotation of traditional Chinese medicine on the five tastes, analyzed the source of pungent-taste herbs, and reviewed the relationship of pungent-taste and nature of the herbs. Using bionic technology of electronic tongue and electronic nose together with chemical separation method, the investigation idea to express the sense of taste-smell and separation characterization of pungent-taste herbs was put forward. At last, we discussed the theory of the pungent herbs on the application in clinical compatibility

Changes of heart-type fatty acid-binding protein in children with chronic heart failure and its significance / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To study serum levels of heart-type fatty acid-binding protein (h-FABP) in children with chronic heart failure (CHF), and the correlation between heart function and the level of h-FABP, with the aim of studying the significance of h-FABP in CHF.</p><p><b>METHODS</b>Thirty-six children with CHF, including 16 cases of endocardial fibroelastosis (EFE) and 20 cases of dilated cardiomyopathy (DCM) were enrolled in the study. Thirty healthy children sevred as the control group. Serum levels of h-FABP were determined using ELISA, and left ventricular ejection fraction (LVEF), cardiac index (CI) and fractional shortening of the left ventricle (LVSF) were measured by two-dimensional echocardiography in the CHF group.</p><p><b>RESULTS</b>Mean levels of h-FABP in the CHF group were significantly higher than in the control group (21.7±4.3 ng/mL vs 6.2±1.7 ng/mL; P<0.01). The worse the heart function, the higher the h-FABP levels (P<0.01). Mean levels of h-FABP in both the EFE and DCM groups were significantly higher than in the control group (P<0.01). Serum h-FABP concentrations were negatively correlated with LVEF, CI and LVSF (r=-0.65, -0.64 and -0.71 respectively; P<0.01) in the CHF group.</p><p><b>CONCLUSIONS</b>Serum h-FABP levels increase in children with CHF and are closely related to the severity of the condition. Serum h-FABP levels can be used as a biomarker for the diagnosis of heart failure and the evaluation of its severity.</p>

Levels of serum brain natriuretic peptide and the correlation to heart function in children with Kawasaki disease / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To study serum levels of brain natriuretic peptide (BNP) in children with Kawasaki disease (KD) and the correlation between BNP levels and the heart function.</p><p><b>METHODS</b>Forty-three children with KD and thirty healthy children were enrolled. Serum levels of BNP were measured using ELISA. KD children received an echocardiographic examination, including measurements of left ventricular ejection fraction (LVEF), left ventricular shorten fraction (LVSF), cardiac index (CI) and left ventricular inflow velocity through the mitral annulus.</p><p><b>RESULTS</b>Mean serum level of BNP at the acute stage in children with KD was significantly higher than that at the recovery stage as well as the control group (p<0.01). The LVEF, LVSF and CI levels at the acute stage were significantly lower than those at the recovery stage in children with KD (p<0.05). The linear regression analysis showed that the BNP level was negatively correlated with the levels of LVEF, LVSF and CI (r=-0.63, -0.52, -0.53, p<0.05).</p><p><b>CONCLUSIONS</b>The serum BNP levels increase significantly in KD children at the acute stage, and are negatively correlated with the levels of LVEF, LVSF and CI. Measurement of serum BNP level is useful for the early diagnosis of KD.</p>

Serum brain natriuretic peptide in children with Kawasaki disease / 世界急诊医学杂志（英文）

BACKGROUND:Kawasaki disease (KD) is a common cause of acquired heart disease in children. Recent studies have focused on the biochemical markers of the myocardium, their high sensitivity and specificity and significance in the diagnosis of KD. This study aimed to determine the serum level of brain natriuretic peptide (BNP) and its relation with the heart function of children with KD and to explore its clinical value in diagnosis of KD. METHODS:Forty-three KD children, aged from 5 months to 8 years (mean 2.3±0.6 years ), were admitted to Qingdao Children's Hospital from February 2007 to April 2009. Among them 27 were male, and 16 female. The 43 patients served as a KD group. Patients with myocarditis, cardiomyopathy, congenital heart disease and other primary heart diseases were excluded. Thirty healthy children, aged from 3 months to 15 years (mean 2.5±0.8 years) or 17 males and 13 females served as a control group. There were no significant differences in age and gender between the two groups (P>0.05). In the KD group, ELISA was used to measure the levels of serum BNP in acute and convalescent stages;and in the control group, the levels of serum BNP were measured once randomly. Left ventricular ejection fraction (LVEF), left ventricular shorten fraction (LVSF), cardiac index (CI) and left ventricular inflow velocity through the mitral annulus (including E-velocity and A-velocity) were measured by two-dimensional echocardiography in the acute and convalescent stages in the KD group. All data were expressed as mean±SD. The methods of analysis included Student's t test and the linear regression analysis test. P<0.05 was considered statistically significant. RESULTS:The level of serum BNP in the acute stage (517.26±213.40) ng/ml was significantly higher than that in the convalescent stage (91.56±47.97) ng/ml in the control group (37.55±7.56) ng/ml (P<0.01). The levels of LVEF, LVSF and CI in the acute stage were significantly lower than those in the convalescent stage (P<0.05), but the E/A level was not significantly different between the acute and convalescent stages (P>0.05). Linear regression analysis showed that the BNP level was negatively correlated with the levels of LVEF, LVSF and CI(r=-0.63, -0.52, -0.53, P<0.05) , but not significantly correlated with the E/A level (r=-0.18, P>0.05). CONCLUSION:The levels of serum BNP are significantly increased in KD patients, and are negatively correlated with the levels of LVEF, LVSF, and CI. The detection of serum BNP level is of clinical significance in the diagnosis of KD.

Levels of serum heart-type fatty acid-binding protein and its clinical significance in children with Kawasaki disease / 中国当代儿科杂志

<p><b>OBJECTIVE</b>This study examined serum level of heart-type fatty acid-binding protein (h-FABP) in children with Kawasaki disease (KD) in order to assess its value in KD.</p><p><b>METHODS</b>Forty children with KD and 30 healthy children were enrolled. Serum levels of h-FABP and cardiac troponin I (cTnI) were measured using ELISA. Serum creatine kinase-MB (CK-MB) level was detected using an autoanalyer. The KD group was classified into two subgroups, with or without coronary artery lesions, based on the findings of the echocardiography.</p><p><b>RESULTS</b>Mean serum levels of h-FABP (18.17+/-13.38 ng/mL vs 6.25+/-1.70 ng/mL; P<0.01) and cTnI (0.27+/-0.22 ng/mL vs 0.11+/-0.02 ng/mL; P<0.05) in the KD group was significantly higher than those in the control group. There was no significant difference in serum CK-MB concentrations between the two groups. Twenty-six patients (65%) and eight patients (20%) showed abnormally increased h-FABP and cTnI levels respectively in the KD group, but none of the control group showed increased levels of both. In the KD group, the percentage of patients with increased h-FABP was significantly higher than those with increased CTnI (P<0.01). The patients with coronary artery lesions had higher serum h-FABP level than those without (28.14+/-14.26 ng/mL vs 11.52+/-6.28 ng/mL; P<0.01).</p><p><b>CONCLUSIONS</b>Serum levels of h-FABP and cTnI increase and can be used as the biomarkers of myocardial damage in children with KD. h-FABP appears to be more sensitive and specific. Detection of serum h-FABP level is useful for diagnosis of KD and coronary artery lesions secondary to KD.</p>

Serum levels of fatty acid-binding protein and brain natriuretic peptide in children with pneumonia complicated by acute congestive heart failure / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To assess the values of serum fatty acid-binding protein (FABP) and brain natriuretic peptide (BNP) in pneumonia complicated by acute congestive heart failure (CHF) in children.</p><p><b>METHODS</b>Serum levels of FABP and BNP were determined using ELISA in 36 children with pneumonia complicated by CHF (pneumonia group) and 28 healthy children (control group).</p><p><b>RESULTS</b>Serum levels of FABP and BNP in the pneumonia group at the acute stage were significantly higher than those in the control group and those at the recovery stage (P<0.01). Compared with the control group, serum levels of FABP and BNP in the pneumonia group at the recovery stage increased significantly (P<0.01). At the acute stage, 35 patients (97.2%) showed increased serum FABP level but 28 (77.8%) showed increased serum BNP level (P<0.05) in the pneumonia group. At the recovery stage, the incidence of abnormal serum FABP (72.2%) was significantly higher than that of BNP (44.4%) in the pneumonia group (P<0.05).</p><p><b>CONCLUSIONS</b>Serum levels of FABP and BNP can be regarded as biochemical markers of myocardial damage in children with pneumonia complicated by CHF and serum FABP appears to be a more sensitive one. Serum FABP and BNP remained at higher levels through the recovery stage, suggesting that myocardial damage existed though the clinical symptoms were improved at the stage.</p>

Efficacy of calf spleen extraction injection combined chemotherapy in the treatment of advanced nonsmall cell lung cancer / 中国癌症杂志

Background and purpose:The occurrence and development of lung cancer are closely correlated with the immune function in the human body.The patients with malignant tumors have shown a disorder of immune function,especially in terms of loss of cellular immune function.The purpose of this study was to investigate the possible auxiliary effect of sipulin in the treatment of advanced non-small-cell lung cancer(NSCLC).methods: Ninety-three patients were randomly divided into two groups:sipulin group:sipulin plus docetaxel+cisplatin;control group:only administered docetaxel+cisplatin.The leukocyte,haemoglobin and platelet,toxicity of digestive tract,body weight,Karnofsky status and efficacy of those patients were evaluated before and after therapy,respectively.Results: Overall response rates were 46.67% and 30.23%(P=0.023)in sipulin group and control group,respectively.The median survival time was 10.1months versus 8.3 months(P=0.035)in sipulin group and control group,respectively.The 1-year survival rate for sipulin group and control group was 52.9% versus 39.4%(P=0.038),respectively.The clinical efficacy and the frequence of leukocyte reduction were better in sipulin group than in control group,the quality of life and clinical symptom of the patients in sipulin group were improved more significantly than those in control group (P

Changes of Serum Cardiac Muscle Enzymogram and Heart Function before and after Interventional Therapy in Ventricular Septal Defect Patients and Its Clinical Significance / 实用儿科临床杂志

Objective To explore the changes of cardiac muscle enzymogram and heart function before and after transactheter closure of ventricular septal defect(VSD).Methods The serum levels of cardiac troponin(cTnI),carcatine kinase(CK),lactie dehydrogenase(LDH),aspartate aminotransferase(AST)and the left ventricular ejection fraction(LVEF)were detected by two-dimensional echocardiography,and the change before and after intervention was analyzed.Results The serum level of cTnI in VSD patients after treatment was markedly higher than that before intervention[(0.29?0.17)?g/L vs(0.02?0.01)?g/L,t=9.0 P0.05).Conclusion Catheterization to treat VSD may have influence on enzymogram and the heart function,which may be related to the minor myocardial damage during the operation.