ABSTRACT
<p><b>OBJECTIVE</b>To investigate the alternation of biological characteristics of residual ear chondrocytes when proliferating in vitro, and study the effects of CDMP-1 on proliferation and differentiation of residual ear chondrocytes.</p><p><b>METHODS</b>Residual ear chondrocytes were cultured in vitro. Then we observed the effect of CDMP-1 on differentiation by immunohistochemistry of collagen type II and dyeing with toluidine blue, investigated proliferation effects of CDMP-1 by method of MIT, and analysis cell cycle changes by flow cytometry.</p><p><b>RESULTS</b>The quantity of GAG gradually decreased along with the increase of the doubling time of chondrocytes in vitro. The quantity of chondrocytes with positive expression of collagen type II were significantly more in the experimental group used with CDMP-1 than the contrast. And the most efficient concentration of CDMP-1 was 100 ng/ml.</p><p><b>CONCLUSIONS</b>CDMP-1 had a good effect on proliferating and could maintain morphology of the residual ear chondrocytes of microtia cultured in vitro.</p>
Subject(s)
Child , Child, Preschool , Female , Humans , Male , Cell Differentiation , Cell Proliferation , Cells, Cultured , Chondrocytes , Cell Biology , Ear Deformities, Acquired , Growth Differentiation Factor 5 , PharmacologyABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibiting action of transforming growth factor-beta type R II receptor(TGF-beta R II) antibody to fibroblasts.</p><p><b>METHODS</b>Four groups of cultured skin fibroblasts were treated with different dosages of TGF-beta R II antibody. Examinations were performed with HYP, MTT, microscope, and flow cytometry techniques.</p><p><b>RESULTS</b>TGF-beta R II antibody resulted in significant inhibition of fibroblasts(P < 0.01).</p><p><b>CONCLUSION</b>TGF-beta R II antibody has inhibiting effects on the proliferation of skin fibroblasts and with a dose-dependent manner.</p>