ABSTRACT
The recent progresses on chemical components and pharmacological activities of the genus Valerianawere summarized.Besides-essential oil, the chemical composition of Valerianais mainly focused on monoterpenoids,sesquiterpenoids,lignans, flavonoids, alkaloids, etc. Iridoids are the main chemical components ofmonoterpenoids. There are two types ofiridoidson the basis of the cyclopentane open or not. The Valerianahas been drawmuch attention for their significant sedation,spasmolysis,antidepression,antitumor, against adenosine A1 receptors and cytotoxicityactivity,and had certain function for cardiovascular disease treatment. Given to the fact of the lack of systematic review and summary of studies on the Valeriana, we summarized and analyze the study literatures on the pharmacological activity of Valerianain recent years, and providedsome basisfor further study.
ABSTRACT
Objective: To establish a method for the quality control of the extracts from the aerial parts of Rheum officinale (EARO) based on multi-assay depending on one determination (MDOD). Methods: The quality control of EARO was carried out through the thin-layer chromatography(TLC), discrimination of chromatographic peak in fingerprint, ultraviolet spectrophotometric, and HPLC methods. The relative correction factors of aloe-emodin, chrysophanol, and physcione for detecting emodin were set up, the factors were used to measure their contents, and the method was adopted to the quality control of EARO. Results: The experimental and control samples in TLC showed the same colored spots at the same position in EARO, five characteristic peaks among six were identified, and showed a good linear relationship at the range of 4.944-29.664 μg/mL (r = 0.9990). The method of MDOD showed the recoveries of aloe-emodin, emodin, chrysophanol, and physcione were 97.08%, 95.78%, 100.60%, and 97.47%, and the RSD values were 3.32%, 2.42%, 3.72%, and 2.67%. There were no significant differences between MDOD method and external standard method. Conclusion: TLC and fingerprint could be used to identify the chemical constituents in EARO, and ultraviolet spectrophotometry could be used to control the quantity of the total anthraquinone. The method of MDOD could be used to quantitatively control the contents of aloe-emodin, emodin, chrysophanol, and physcione, and the method is simple and accurate, which has a higher repeatability.
ABSTRACT
OBJECTIVE: To optimize the process of extacting effective constituents from aerial part of Rheum officinale Baill by Plackett-Burman design combined with CCD response surface methodology. METHODS: In Plackett-Burman combined with CCD response surface design, the independent variables were concentration of ethanol, extraction time, and solvent fold. The dependent variables were contents of total anthraquinone and rheum emodin. RESULTS: The optimal extraction process of total anthraquinone was as follows: 67.25% ethanol, 90 min for reflux per time, 28:1 fold of solvent and 2 times of extraction; the optimal extraction process of emodin was 85% ethanol, 90 min for reflux per time, 40 fold of solvent and 2 times of extration. CONCLUSION: The optimal process is simpleand convenient for extracting aerial part of Rheum officinale Baill with high precision and predictability. Copyright 2012 by the Chinese Pharmaceutical Association.