ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of neuregulin (neural regulation of protein, NRG) in the process of mouse spermatogonia proliferation.</p><p><b>METHODS</b>Mouse testis fragments were cultured in the medium DMEM containing purified NRG1beta or NRG3 at the concentration of 50, 100 and 200 ng/ml, respectively, followed by BrdU immunohistochemical staining and determination of the proliferation rate of spermatogonia.</p><p><b>RESULTS</b>Compared with the control group, neuregulin significantly promoted the proliferation of spermatogonia (P < 0.05). The proliferation rates of spermatogonia cultured in the medium with 50, 100 and 200 ng/ml of NRG13 were 1.69, 1.55 and 1.86 times, and those with 50, 100 and 200 ng/ml of NRG3 were 1.35, 1.54 and 2.11 times that of the control.</p><p><b>CONCLUSION</b>NRG1beta and NRG3 can promote the proliferation of mouse spermatogonia, and NRG is expected to be applied in the treatment of male infertility.</p>
Subject(s)
Animals , Male , Mice , Cell Proliferation , Cells, Cultured , Intracellular Signaling Peptides and Proteins , Pharmacology , Mice, Inbred C57BL , Neuregulin-1 , Pharmacology , Signal Transduction , Spermatogonia , Cell Biology , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To develop a new method for the detection of male human papillomavirus (HPV) genotypes and to investigate its clinical application value.</p><p><b>METHODS</b>With computer assistance and based on the classical common primers MY09/11, modified PGMY09/11 with 23 HPV subtypes for PCR and Genbank data on HPV, we designed probes for the simultaneous detection of 18 high-risk subtypes (HPV-16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 73, 83 and MM4) and 5 low-risk subtypes (HPV-6, 11, 42, 43 and 44) and fixed them to the special membrane to make a DNA chip. A total of 112 male urethral samples were collected with swabs and studied for the clinical value. Meanwhile the single subtypes of HPV positive were sequenced and the standard samples detected for their sensitivity.</p><p><b>RESULTS</b>Of the total number, 25 samples were found to be HPV positive, 13 single HPV infection and 12 multiple infection. Nine HPV gene subtypes were noted in the samples: 6, 11, 16, 18, 33, 35, 43, 56 and 73, with sensitivity up to 10 copies of HPV DNA.</p><p><b>CONCLUSION</b>Human papillomavirus genotyping by the membrane DNA chip is applicable to the diagnosis of male HPV infection as well as to the related epidemic and etiological investigation.</p>