Analysis on epidemic of human brucellosis in Jiangxi Province from 2011 to 2017 / 中华疾病控制杂志

Objective To investigate the epidemic characteristics of human brucellosis in Jiangxi province from 2011 to 2017，thereby providing reference for future prevention and control work. Methods We analyzed the case information reported in the National Notifiable Infectious Diseases Reporting Information System of Jiangxi Province during 2011-2017 by combining with the monitoring data. Results A total of 239 cases were recorded over these six years, with an average incidence rate of 0.08/100 000. Most cases and agglomerations were concentrated in the Nanchang area (48 cases/4 cases), with the case distribution ranging from 1 in 2011 to all in 2016, and the number of counties(districts) involved had increased from 1 in 2011 to 68 in 2017, which increased rapidly year by year ( 2= 94.31, P<0.001). The gender ratio was 3.12 ∶〗1(181 ∶〗58). Most cases were concentrated in the 40-65 age group, while farmers and herdsmen were high-risk population, accounting for 59.83 % (143/239). The incidence of brucellosis peaked from May to September (May peak and September peak). In 2012-2017, 7 160 blood samples were collected from risk population, with 95 being positive for Brucella (1.33 %). From 2013 to 2017, 62 strains of the Brucella, 4 were Brucella melitensis type1, 54 were Brucella melitensis type3 and 4 were Brucella suis were isolated from 193 cases. Conclusions The human brucellosis was aggravated in Jiangxi province, with the expansion of regional scope. Therefore, more efforts should be focused on regulation to prevent and control brucellosis better.

Effect of p38-MAPK and STAT3 on biological properties of DPSCs under different oxidative stress / 中国免疫学杂志

Objective: To investigate the effect on the proliferation, mineralization and adipogenic differentiation of dental pulp stem cells ( DPSCs) under different oxidative stress by p38-MAPK and STAT3 signaling pathway. Methods: DPSCs was isolated and identified from pulp tissue by trypsin digestion. The CCK8 detection cell model was divided into oxygen group, 3％ O2 group, 6％ O2 group, 9％ O2 group firstly, each group included the SH-4-54 treatment group, and the SB203580 treatment group, a total of 12 groups. Cell proliferation was detected by CCK8 test for 6 h, 12 h, 24 h, 36 h, 48 h. We observed the differentiation of DPSCs mineralization by alizarin red staining involved in p38-MAPK and STAT3 signaling pathway, and the a-dipogenic differentiation of DPSCs by oil red O staining. Results: DPSCs were cultured by the enzyme digestion method, flow cytometry showed that DPSCs spec-ific marker Stro-1 expression was 96. 85％, CD146 positive expression was 90. 94％, the positive low expression of CD45 was 1. 02％, the positive low expression of CD34 was 40. 76％, p38-MAPK inhibited DPSCs with different oxygen environment. The proliferation of STAT3 promotes the survival of groups DPSCs, the p38-MAPK signaling pathway plays a positive role in the differentiation of mineralization of DPSCs and the STAT3 signaling pathway plays negative regulation role, the two signaling pathway play opposite effects in adipogenic differentiation research. Conclusion: The proliferation rate of DPSCs is higher in normoxic environment, and the STAT3 signal pathway can promote the proliferation of DPSCs, the MAPK signal pathway promotes the odontoblastic differentiation of DPSCs.

Regulatory effect of CGRP on NOS in tibial fracture healing in the rat model of highly selective sensory and motor denervation / 医学研究生学报

Objective The aim of this study was to explore the regulatory effect of calcitonin gene-related peptide (CGRP) on nitric oxide synthase (NOS) and the nitric oxide (NO) pathway in tibial fracture (TF) healing in the rat model of highly selective denervation.Methods A total of 60 SD rats were randomly divided into three groups of equal number, TF control, TF + sensory denervation (SD), and TF + motor denervation (MD). At 1, 2, 3 and 4 weeks after modeling, osteotylus samples were obtained from the rats for observation of the bone morphology and determination of the expressions of CGRP and NOS by immunohistochemistry and HE staining.Results At 2 and 3 weeks after modeling, the rats of the TF+SD group, as compared with the TF controls, showed significantly decreased expression of CGRP (0.150±0.014 vs 0.210±0.013, P<0.05; 0.143±0.017 vs 0.203±0.013, P<0.05) and that of eNOS in the osteotylus (0.170±0.016 vs 0.219±0.026, P<0.05; 0.158±0.016 vs 0.201±0.013, P<0.05).Conclusion Selective denervation, especially sensory denervation, may change the expression of CGRP and thereby that of NOS in the osteotylus of the rat with tibial fracture, which consequently affects the growth of the osteotylus and fracture healing as well.

A retrospective analysis of 6 children with Duchenne muscular dystrophy / 中国当代儿科杂志

<p><b>OBJECTIVE</b>To analyze the clinical features of 6 children with Duchenne muscular dystrophy (DMD) and review related literature, and to provide a basis for early diagnosis and effective treatment of this disease.</p><p><b>METHODS</b>A retrospective analysis was performed on the clinical data of 6 children with DMD who were admitted to the First Affiliated Hospital of Nanjing Medical University from January 2010 to October 2015.</p><p><b>RESULTS</b>All the 6 cases were boys without a family history of DMD, and the age of diagnosis of DMD was 1.2-11.5 years. All patients had insidious onset and increases in alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, α-hydroxybutyrate dehydrogenase, creatine kinase (CK), and creatine kinase-MB, particularly CK, which was 3.3-107.2 times the normal level. Their gene detection results all showed DMD gene mutation. The gene detection results of two children's mothers showed that they carried the same mutant gene. The muscle biopsy in one case showed that the pathological changes confirmed the diagnosis of DMD. The level of CK in one case declined by 77.0% 5 days after umbilical cord blood mesenchymal stem cell transplantation.</p><p><b>CONCLUSIONS</b>For boys with abnormal serum enzyme levels and motor function, DMD should be highly suspected. It should be confirmed by CK and DMD gene detection as soon as possible. And the progression of the disease could be delayed by early intervention for protecting the remaining normal muscle fibers.</p>

Effect of celecoxib on pulmonary hypertension of chronic hypoxia and hypercapnic rats / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To study the effect of celecoxib on chronic hypoxia and hypercapnic pulmonary hypertension.</p><p><b>METHODS</b>SD rats were randomly divided into normal control group (A), hypoxic hypercapnic group (B), hypoxic hypercapnia+ celecoxib group (C). The content of TXB2 and 6-keto-PGF1alpha in plasma and lung were detected by the technique of radioimmunology.</p><p><b>RESULTS</b>(1) Mean pulmonary arteria pressure(mPAP) was significantly higher in rats of B group than those of A group. mPAP was significantly higher in rats of C group than those of B group. Differences of mPAP were not significant in three groups. (2) The content of TXB2 in plasma and lung and the ratio of TXB2/6-keto-PGF1alpha were significantly higher in rats of B group than those of A group. The ratio of TXB2/6-keto-PGF1alpha was significantly higher and the content of 6-keto-PGF1alpha in plasma and lung was significantly lower in rats of C group than those of B group. (3) Light microscopy showed that WA/TA (vessel wall area/total area) and PAMT (the thickness of medial smooth cell layer) were significantly higher in rats of B group than those of A group. WA/TA and PAMT were significantly higher in rats of C group than those of B group. (4) Electron microscopy showed the thickening of vessel wall and the proliferation of collagen fiber in B group and augmentation of smooth muscle cell and abundance of myofilament in pulmonary arterioles in C group.</p><p><b>CONCLUSION</b>Celecoxib can aggravate hypoxic hypercapnia pulmonary hypertension and pulmonary vessel remodeling by increasing the ratio of TXA2/PGI2.</p>

Antagonistic effect of 3'-daidzein sulfonate sodium on prostatic hyperplasia in mice / 中华男科学杂志

<p><b>OBJECTIVE</b>To study the antagonistic effect of 3'-daidzein sulfonate sodium (DSS) on benign prostatic hyperplasia (BPH) and its possible mechanism.</p><p><b>METHODS</b>Forty healthy mice were randomly divided into five groups: a normal control group without any treatment, a model group of BPH treated by subcutaneous injection of testosterone propionate, a positive control group with the BPH procedure treated by Qianliekang, a 20 mg/(kg x d) DSS group with the BPH procedure and a 40 mg/(kg x d) DSS group with the BPH procedure. After 12 days of the above treatments, the mice were sacrificed for measurement of the prostate glandular wet weight, the index of prostate gland (PI), the morphological changes of prostate gland by light microscopy and the contents of testosterone and estradiol in the serum.</p><p><b>RESULTS</b>The prostate wet weight and PI decreased dose-dependently after DSS treatment for 12 days compared with the BPH model group (P < 0.05 or P < 0.01). The hyperplastic epithelioglandular papilla waned and even disappeared in the DSS treated groups under the light microscope, the epithelial cells became cubical or flat. The effect of DSS at 40 mg/(kg x d) was similar to that of the positive anti-BPH drug Qianliekang. DSS reduced the serum testosterone, estradiol contents and the T/E2 ratio (P < 0.05 or P < 0.01).</p><p><b>CONCLUSION</b>DSS has significant antagonistic effect on BPH induced by testosterone propionate in mice, which may involve its regulatory action on the sex hormone balance.</p>

DELETION ANALYSIS OF DNA FRAGMENT RM07 FROM HALOBACTERIUM HALOBIUM / 微生物学通报

The DNA fragment RM07 was isolated from halophilic archaea Halobacterium halobium, which can function as promoter not only in halophilic archaea, but also in Escherichia coli as eubacterial promoter. Sequencing analysis indicated that it possessed the typical consensus sequences (-35 and -10) of bacterial gene promoter, which was confirmed by further deletion analysis: With its -35 sequence deleted and -10 sequence left,DNA fragment RM07a nearly cannot initiate transcription;With its both -35 and -10 sequences,RM07b DNA fragment could be active as promoter at a level even higher than RM07. Our research also showed that the promoter function of RM07 fragment in Escherichia coli was under the control of environmental factors,especially its positive correlation with the increasing concentration of sodium chloride. Therefore, RM07 DNA fragment may be potential1 novel promoter source for constructing double-function vectors. It also has special significance in elucidating the issues of the fusing characteristics of archaea and lateral gene transfer between archaea and bacteria.