ABSTRACT
<p><b>OBJECTIVE</b>To explore the method of inducing and building pancreatic cancer cell sublines with radiation resistance.</p><p><b>METHODS</b>Simulating the clinical radiotherapy, the pancreatic cell lines SW1990, Capan-1 (Cap), AsPC-1 (ASPC), P3, PANC-1 (Pan-1) and MIAPaCa-2 (MIA) were repeatedly given individual dose of X-rays with liner accelerator to induce radiation resistance, the changes of cell morphology, cell cycle and radio sensibility in the induced cell lines were compared with the parental cell lines at the end of inducing course.</p><p><b>RESULTS</b>Compared with the parental cells, there were significant changes in morphology in the pancreatic cancer cell sublines after the radiation. Cell cycle analysis suggested that SW1990-R, ASPC-R, MIA-R, PAN-R and P3-R had lower G(2)/M and greater SF(2) (survival fraction after 2 Gy irradiation) compared with the parental cell lines.</p><p><b>CONCLUSIONS</b>The method of radiating cells step by step and repeatedly is viable to establish radio-resistant pancreatic cancer cell lines.</p>