ABSTRACT
Abstract Objectives Long non-coding RNAs (LncRNAs) act as an indispensable role in the Preeclampsia (PE)-related trophoblast function, while its relationship with Small Nucleolar RNA Host Gene 22 (SNHG22) remains unknown. Hence, this study aimed to investigate the roles of lncRNA SNHG22 in the Preeclampsia (PE)-related trophoblasts function and the underlying mechanism. Methods Normal placentas and placentas from PE patients were collected to detect the expression of lncRNA SNHG22. Then, trophoblasts HTR-8/Svneo and JEG-3 were purchased, cultured, and treated to investigate the roles of lncRNA SNHG22 on cell migration and invasion as well as its underlying regulatory mechanism. Results The SNHG22 was downregulated in PE patients, and it was found that SNHG22 overexpression could drive migration and invasion of trophoblasts, while SNHG22 depletion exerted a suppressive effect. Mechanistically, SNHG22 was validated to regulate microRNA-128-3p (miR-128-3p), and Protocadherin 11 X-Linked (PCDH11X) was identified as the target gene of miR-128-3p. Furthermore, it was found that SNHG22 acted as a promoter in the migration and invasion of trophoblast cells in a miR-128-3p/PCDH11X dependent manner, and SNHG22 silencing weakened the activation of PCDH11X-mediated PI3K/Akt signaling pathways through inhibiting miR-128-3p, thereby preventing migration and invasion of trophoblasts. Conclusion SNHG22 acted as a driver in the migration and invasion of trophoblasts and may be considered a candidate for the amelioration of PE.
ABSTRACT
Our study aimed to explore the relevant risk factors for intrauterine death of fetuses in the third trimester of pregnancy via a retrospective analysis. Then, 98 pregnant women with intrauterine death of fetuses in the third trimester of pregnancy were enrolled, who had undergone the induced labor of dead fetuses in our hospital from January, 2013 to January, 2015. By taking their disease conditions into considerations, methods of induced labor as softening of cervix with dinoprostone suppositories and amniotic infusion of ethacridine or oxytocin were performed, and the timely cesarean section for termination of pregnancy was performed. After the treatment, a detailed medical history was recorded, including their family history, past history and conditions of this pregnancy. Besides, autopsy of dead fetuses and pathological examinations were performed as well as chromosome examinations of the placenta, the umbilical cord and the fetal membrane in an attempt to identify the relevant factors for causes of death, so as to do a good job in the post-natal consultation. The induced labor procedures were successfully performed on all pregnant women, and the investigation of causes of intrauterine death showed that placental factors were responsible for the largest proportion of all causes of intrauterine death in single [28 patients], which was 31.82%, including 13 patients with placenta praevia and 13 with placental abruption. The secondary factors were umbilical cord factors, accounting for 30.68%. Among the factors of pregnant women, gestational hypertension occurred in 7 patients, accounting for 58.33% of factors of pregnant women. While among the causes of intrauterine death in twins, umbilical cord factors were found to be the main causes of death, accounting for 30.00%, followed by placental factors and factors of pregnant women, which accounted for 20.00%, respectively. Placenta factors, umbilical cord factors and factors of pregnant women were the main causes of intrauterine death of fetuses in the third trimester of pregnancy. Therefore, pregnancy tests on time for routine screening is recommended for pregnant women so as to identify the potential risk factors in time and actively carry out symptomatic treatment, thereby reducing the chances of intrauterine death of fetuses and improving the qualities of pregnancy. It is worth clinical attention