Multi-component content determination of Dracocephalum tanguticum by quantitative analysis of multi-components by single-marker / 中国中药杂志

This study aims to establish a method for the simultaneous determination of 7 active components in Dracocephalum tanguticum and to evaluate the quality of medicinal materials from different habitats. The method was established with high performance liquid chromatography(HPLC) and the gradient elution was performed with the mobile phase of acetonitrile-methanol-0.2% phosphoric acid solution at a column temperature of 35 ℃, an injection volume of 15 μL, and a flow rate of 0.6 mL·min~(-1). The detection wavelength was set as 215 nm. With rosmarinic acid as the internal reference, the relative correction factors and the content of other 6 components were calculated. The results were compared with those obtained with the external standard method. The results showed that the samples from Huangzhong county, Qinghai province had the best quality, with the highest content of p-hydroxybenzoic acid, cosmosiin, rosmarinic acid, oleanolic acid, and ursolic acid(9.29, 12.14, 6.02, 3.11, 17.67 mg·g~(-1) respectively). The samples from Chaya county, Tibet autonomous region ranked the second, with the highest content of betulin and betulinic acid(15.53, 7.17 mg·g~(-1), respectively). The method is accurate, reliable, and repeatable and suitable for the simultaneous determination of multiple components in D. tanguticum. The content of functional components varied in the samples from different producing areas and can be used as the indicator for the quality evaluation of medicinal materials.

Clinical efficacy of alprostadil combined with butylphthalide on patients with acute ischemic stroke and its effect on hs-CRP / 药物评价研究

Objective To investigate the clinical efficacy of alprostadil combined with butylphthalide on patients with acute ischemic stroke and its effect on hs-CRP.Methods Eighty-five patients with acute ischemic stroke admitted into our hospital were divided randomly into observation group (n =42) and control group (n =43).Patients in control group were treated with alprostadil,and those in observation group were treated with alprostadil combined with butylphthalide.The neurologic impairment score (NIHSS),ADL score,hs-CRP,coagulation function,and clinical efficacy in the two groups were observed and compared.Results After 14 days of treatment,the NIHSS and ADL score in both groups were improved,and NIHSS in observation group were much lower than control group,ADL were much higher,and hs-CRP were markedly lower than that of control (P ＜ 0.05).And the coagulation function indexes in both groups were obvious lowered,and DD and FIB in observation group were conspicuously lower than those in control group,and PT were much higher (P ＜ 0.05).In addition,observation group with effective rate of 90.5％ were significantly higher than that of control group with 72.1％ (P ＜ 0.05).Conclusion Compared with only alprostadil treatment,alprostadil combined with butylphthalide had a better capability of treating patients with acute ischemic stroke by improving coagulation function and neurologic function,and inhibiting levels of hs-CRP.

Clinical efficacy of alprostadil combined with butylphthalide on patients with acute ischemic stroke and its effect on hs-CRP / 药物评价研究

Objective To investigate the clinical efficacy of alprostadil combined with butylphthalide on patients with acute ischemic stroke and its effect on hs-CRP.Methods Eighty-five patients with acute ischemic stroke admitted into our hospital were divided randomly into observation group (n =42) and control group (n =43).Patients in control group were treated with alprostadil,and those in observation group were treated with alprostadil combined with butylphthalide.The neurologic impairment score (NIHSS),ADL score,hs-CRP,coagulation function,and clinical efficacy in the two groups were observed and compared.Results After 14 days of treatment,the NIHSS and ADL score in both groups were improved,and NIHSS in observation group were much lower than control group,ADL were much higher,and hs-CRP were markedly lower than that of control (P ＜ 0.05).And the coagulation function indexes in both groups were obvious lowered,and DD and FIB in observation group were conspicuously lower than those in control group,and PT were much higher (P ＜ 0.05).In addition,observation group with effective rate of 90.5％ were significantly higher than that of control group with 72.1％ (P ＜ 0.05).Conclusion Compared with only alprostadil treatment,alprostadil combined with butylphthalide had a better capability of treating patients with acute ischemic stroke by improving coagulation function and neurologic function,and inhibiting levels of hs-CRP.

Evaluation of left ventricular function and twist in patients with diabetic cardiovascular autonomic neuropathy by speckle tracking imaging / 中华心血管病杂志

<p><b>OBJECTIVE</b>To evaluate left ventricular (LV) function and twist in patients with diabetic cardiovascular autonomic neuropathy (CAN) by two-dimensional speckle tracking imaging (STI).</p><p><b>METHODS</b>STI was performed in 56 subjects with type 2 diabetes mellitus (DM) (35 with DM only: group A, 21 with CAN: group B) and 34 normal subjects (Control) from LV short-axis view. LV peak systolic, peak early (E') and peak late (A') diastolic circumferential strain in 18 myocardial segments were measured at the levels of mitral annulus, papillary muscle and apex and the rotation at mitral annulus and apex levels were also measured. LV peak systolic and the ratio of E' and A' of global and three levels, twist, untwisting rate and untwisting half-time were calculated.</p><p><b>RESULTS</b>In group A, compared with control group, LV peak systolic radial circumferential strain has no significant difference (P > 0.05), E'/A' was reduced (P < 0.05), twist at aortic valve closure and twist at mitral valve opening were significantly increased (P < 0.05), untwisting rate reduced, and untwisting half time delayed. In group B, compared with control group and group A, circumferential strain parameters [(-12.64 ± 6.49)% vs. (-19.11 ± 9.98)% and (-21.14 ± 10.13)%, P < 0.05] and E'/A' [(0.90 ± 0.35) vs. (1.24 ± 0.47) and (1.98 ± 0.63), P < 0.05] were significantly decreased, twist at aortic valve closure [(19.08 ± 5.62)° vs. (16.57 ± 2.84)° and (14.36 ± 4.06)°, P < 0.05] and twist at mitral valve opening [(13.99 ± 2.31)° vs. (11.36 ± 2.63)° and (9.04 ± 5.63)°, P < 0.05] were significantly increased, untwisting rate [(0.40 ± 0.28)%/ms vs. (0.46 ± 0.14)%/ms and (0.53 ± 0.21)%/ms, P < 0.05] reduced, and untwisting half time [(489.61 ± 97.14) ms vs. (445.21 ± 54.53) ms and (410.60 ± 50.23) ms, P < 0.05] delayed.</p><p><b>CONCLUSION</b>Speckle tracking imaging could be used to evaluate early changes on LV twist deformation and LV systolic function in patients with type 2 diabetes mellitus.</p>

Analysis of the character of self-adaptation of nasal structure in patients with nasal septum deviation / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To analyze the different characters of nasal airflow-field between 10 patients with nasal septum deviation and 20 healthy Chinese people by the method of three-dimensional reconstruction of these people's nasal cavity and numerical simulation of the flow field in these nasal cavity models. The character of airflow-field was considered by analyzing the relationship between the structure and function of the human nose.</p><p><b>METHODS</b>Based on the data obtained from the CT images, 10 patients with nasal septum deviation and 20 healthy Chinese people's nasal cavity models were reconstructed by the method of surface rendering. The flow field in these three-dimensional models was simulated with finite element method. The different characters of nasal airflow-field was analysed between two groups of people.</p><p><b>RESULTS</b>The airflow distribution in the nasal cavity model could be acquired from the simulation results of the velocity. The airflow for patients with nasal septum deviation mainly passed through the broad nasal cavity, especially in the middle part of meatus of nose. In the healthy people group, the airflow mainly passed through the main side of the nasal cavity, especially in the middle and inferior part of the meatus of nose. The pressure value at any point in the nasal cavity model could be obtained from the results of the pressure plot. In the patients with nasal septum deviation, the pressure mainly dropped in the part of the nasal septum deviation, accounting approximately 71.36% of the total pressure drop. In the group of healthy people, the pressure dropped mainly in the limen nasi, accounting approximately 58.78% of the total pressure drop. The nasal airway resistance of the patients with nasal septum deviation was larger than that in the group of healthy people.</p><p><b>CONCLUSIONS</b>The three-dimensional nasal airway can reflect the characters of the human nasal airway. It can be used to analyze the change of the aerodynamic in nasal cavity caused by the abnormal anatomy of the nose. This experiment can proof that human nose has the function of self-adaptation, it can build a foundation for the construction of the model of self-adaptation of the human nose.</p>

Clinical analysis of massive hemispheric infarction treated with moderate hypothermia / 中华创伤杂志(英文版)

The potential of hypothermia in reducing neuronal damage has been demonstrated in several animal models of focal cerebral ischemia. The feasibility and safety of this technique for acute stroke has been only examined in one study, describing a total of 25 patients. We analyzed the data from 50 consecutive patients with acute stroke treated with moderate hypothermia in neurocritical care units of 4 university clinics to evaluate the feasibility and safety of moderate hypothermia.