ABSTRACT
Objective To evaluate the regulatory effect of the adaptor related protein complex 2 subunit μ1(AP2M1)on proliferation and invasion of diffuse large B-cell lymphoma(DLBCL).Methods Human diffuse large B-cell lymphoma cell line OCI-LY8 was aliquoted into control group,NC-shRNA group,AP2M1-shRNA group,NC-LV group,and AP2M1-LV group.Lipofectamine 2000 was used for cell transfection.Cell proliferation was detected by tetramethylazolium salt(MTT)method,apoptosis was detected by flow cytometry and cell migration and invasion were detected by Transwell assay.The protein expression of AP2M1,epidermal growth factor receptor(EGFR),p-phosphatidylinositol 3 kinase(PI3K),PI3K,p-protein kinase B(Akt)and AKT was detec-ted by Western blot.Results Compared with control group,the relative expression of AP2M1 mRNA and protein in the AP2M1-shRNA group was decreased(P＜0.05).The relative cell viability was increased(P＜0.05).The cell apoptosis rate was decreased(P＜0.05).The counting number of migrating and invading cells was in-creased(P＜0.05).The relative expression level of EGFR protein and the phosphorylation level of PI3K and AKT were increased(P＜0.05).Compared with Control group,the expression of AP2M1 mRNA and protein relative ex-pression level in AP2M1-LV group was increased(P＜0.05).The relative cell viability was decreased(P＜0.05).The cell apoptosis rate was increased(P＜0.05).The number of migrating and invading cells was decreased(P＜0.05).The relative expression level of EGFR protein and the phosphorylation level of PI3K and AKT were all decreased(P＜0.05).Conclusions The over-expression of AP2M1 partially inhibits the proliferation and invasion of DLBCL cells by inhibiting the EGFR/PI3K/AKT signaling pathway.
ABSTRACT
Objective To investigate the epidemiological characteristics of hospitalized hypertension patients with different metabolic abnormalities associated with cardiovascular disease.Methods 1 843 patients with diagnosis of hypertension were selected,including 327 patients with metabolic syndrome as the research object.Metabolic syndrome of different age,gender,abnormal components epidemic status were analyzed.Results Positive rates of overweight,dyslipidemia,hyperglycemia,cardiovascular disease in 327 patients were 42.20％,37.92％,35.17％,71.25％,the highest positive rate for cardiovascular disease was significantly higher than the other three indexs(x2 =56.22,73.28,85.51,all P ＜ 0.05).There was no significant differences in the rates of overweight between men and women (x2 =0.42,P ＞ 0.05).Dyslipidemia and hyperglycemia incidence of women were significantly more than those of men,there was statistically significant difference (x2 =5.73,6.47,all P ＜ 0.05).The incidence of cardiovascular disease in males was high than that in females (x2 =24.66,P ＜ 0.05).Conclusion Preventive different metabolic abnormalities need to take different measures according to age and gender.Weight control,blood lipids and blood sugar are the key to the prevention of cardiovascular disease.