ABSTRACT
Objective To increase the sensitivity of quantitative PCR using DNA polymerase in combination with aptamer 6-10.Methods The quantitative PCR was performed using DNA polymerase mixed with aptamer 6-10 or specific antibody.The low detection limit of each modified method was compared with that of the conventional method.Results The quantitative PCR using aptamer 6-10(200 nmol/L) or specific antibody increased the low detection limit of human death receptor 5(DR5) plasmid from 105 copies/?l to 103 copies/?l.Melting curves showed that each method had minimal nonspecific amplification when the concentration of DR5 plasmid was 105 copies/?l;however,when the concentration of DR5 plasmid was 103 copies/?l,the conventional method had nonspecific amplification,whereas the method using aptamer 6-10 had minimal nonspecific amplification.Conclusions Aptamer 6-10 of DNA polymerase can increase the sensitivity of quantitative PCR.