ABSTRACT
Objective: To study the chemical components of Citrus medica L.var. sarcodactylis Swingle and to supply evidence for the quality control. Methods: Chromatography and recrystallization methods were applied to analyze the components. Results and Conclusion: Three compounds were extracted from Citrus medica L. var. sarcodactylis (Noot) Swingle. And their structures were identified as Limettin (I), stigmasta-5,22-dien-3-ol (II) and palmitic acid (III) by the methods of UV, MS, IR, 1HNMR, 13CNMR and DEPT. Stigmasta-5,22-dien-3-ol (II) was obtained by extraction.
ABSTRACT
Objective To determine the content of 5,7-dimethoxycoumarin in Fructus Citris Sarcodactylis. Methods HPLC was used. Mobile phase was methanol-water(65 ∶35),detection wavelength at 326 nm,flow rate 1mL/min and column temperature at 35 ℃.Results The linearity of 5,7-dimethoxycoumarin was in the range of 0.3528～1.7640 ?g. The regression equation was Y=2403.73 X-65.35,r=0.9998. The average recovery was 99.33 %,RSD=1.47 %(n=6). Conclusion The method is simple and accurate. It can be applied in the determination of 5,7-dimethoxycoumarin in Fructus Citris sarcodactylis.