ABSTRACT
Objective To study the biological characteristics of the human breast cancer cell mammospheres (MSs) ,and con‐struct breast cancer stem cell experiment model .Methods MCF‐7 cells were cultured in the serum‐free media supplemented with growth factors (the MCF‐7 group) ,and the MSs was collected (the MSs group) .The migration ,invasive and animal tumor forma‐tion abilities of MSs were detected by wound healing ,transwell invasive assay and animal tumor formation test .Results The wound line of MSs healed after 48 hours ,but the line of MCF‐7cells could not heal after 48 h .The number of the cells going through the membrane in MSs group was (76 .24 ± 0 .35) ,and the number in MCF‐7cells was (17 .38 ± 0 .18)(P<0 .05) .MSs had stronger ani‐mal tumor formation ability than MCF‐7 cells .Conclusion MSs have stronger abilities in migration ,invasive and animal tumor for‐mation ,and could be used in the studies of breast cancer stem cell as experimental model .
ABSTRACT
Objective To study the effect of partial parenterai nutrition support in severe cases of general surgery.Methods Treated 170 severe cases from January 2001 to September 2008,ran two sets, 80 cases of ex-perimental group and 90 cases of the control group.Results The exaltation of hemoglobin, albumin, lymphocytes and weight in experimental group were better than those in the control group (P < 0.05).The level of glycemic control was also better than that in the control group (P < 0.05).Conclusions The effection of partial parenteral nutrition support is reliable, practical and low-cost.