Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 3 de 3
Filter
Add filters








Language
Year range
1.
China Occupational Medicine ; (6): 470-474, 2020.
Article in Chinese | WPRIM | ID: wpr-881927

ABSTRACT

OBJECTIVE: To establish a headspace gas chromatography-mass spectrometry method for the determination of 14 chlorinated hydrocarbons in urine. METHODS: The urine sample 4.00 mL and anhydrous sodium sulfate 3.00 g were added into a 10.00 mL headspace bottle, then the headspace bottle was maintained at 70 ℃ for 40.0 min. After headspace pretreatment, 14 chlorinated hydrocarbons in headspace air were separated in the DB-5 MS capillary column of the gas chromatography and detected by mass spectrometer. RESULTS: There was a good linear relationship of 14 chlorinated hydrocarbons in urine in the range of 0.62-1 630.00 μg/L. The linear correlation coefficient was greater than 0.999 0.The minimum detectable concentration was 0.19-0.43 μg/L and the minimum quantitative concentration was 0.62-1.44 μg/L. The average recovery rate was 89.8%-107.1%. The within-run relative standard deviation(RSD) was 4.0%-8.5% and the between-run RSD was 6.3%-9.1%. Urine samples can be stored at 4 ℃ or-8 ℃ for 3 days and below-20 ℃ for 7 days. CONCLUSION: This method is rapid, simple, sensitive, accurate and has little interference,which can be used as a method for detecting 14 kinds of chlorinated hydrocarbons in urine samples of patients with occupational poisoning.

2.
China Occupational Medicine ; (6): 219-222, 2018.
Article in Chinese | WPRIM | ID: wpr-881690

ABSTRACT

OBJECTIVE: To establish a method for detecting human urinary thiocyanate by gas chromatographic and pre-column derivatization with 2,3,4,5,6-pentafluorobenzyl bromide( PFB-Br). METHODS: A total of 20. 0 μL of urine was taken and 1. 0 m L of acetonitrile and 100. 0 μL of PFB-Br were added for derivative reaction. The gas chromatography was directly used for measurement. RESULTS: The urinary thiocyanate concentration showed a good linear range of 1. 000-10. 000 mg/L. The linear correlation coefficient was 0. 999 6. The minimum detection concentration was 0. 112 mg/L,and the minimum quantitative concentration was 0. 411 mg/L( 20. 0 μL urine sample). The standard recovery rate was 97. 22%-102. 04%.The within-run relative standard deviation( RSD) of this method was 1. 56%-5. 35%. The between-run RSD was 1. 46%-5. 10%. Hydrocyanic acid ions interfered with the measurement. Other common inorganic ions such as chloride,sulfate,and nitrate ions did not interfere with the measurement results. The samples can be stored at 4 ℃ for at least 15 days. CONCLUSION: This method is suitable for detecting human urinary thiocyanate.

3.
China Occupational Medicine ; (6): 223-226, 2018.
Article in Chinese | WPRIM | ID: wpr-881691

ABSTRACT

OBJECTIVE: To establish a method for simultaneous detection of five kinds of olefine chlorides in workplace air by solvent desorption-gas chromatography. METHODS: The olefine chlorides in workplace air were collected using activated charcoal tubes,desorbed by carbon disulfide,separated on a HP-INNOWAX capillary column and detected with flame ionization detector. RESULTS: The linear range of the five kinds of chlorinated olefins( vinyl chloride,allyl chloride,chloroprene,trans-1, 2-dichloroethylene, cis-1, 2-dichloroethene) was wide and the linear correlation coefficient was greater than 0. 999 8. The detection limit and the minimum detectable concentrations were 0. 30-0. 80 mg/L and 0. 05-0. 13 mg/m~3,respectively( air sample volume 6. 0 L). The recovery rate was 95. 90%-102. 67%. The within-run and betweenrun relative standard deviations were 0. 00%-3. 74% and 0. 21%-6. 12%,respectively. The samples could be preserved at room temperature for at least 14 days. CONCLUSION: This method has simple operation,good linear range,high sensitivity and good reproducibility,which is suitable for detecting 5 kinds of olefine chlorides in workplace air.

SELECTION OF CITATIONS
SEARCH DETAIL