Villus cell culture and its karyotype analysis for 112 women who had spontaneous abortion in early pregnancy / 国际检验医学杂志

Objective To investigate the clinical relationship between villus cells chromosome abnormality and spontaneous a-bortion in early pregnancy.Methods Villus cells from 112 women who just had spontaneous abortion in early pregnancy were cul-tured and prepared for karyotype analysis.Results 105 specimens were anaIyzed successfully which accounted for 93.75%(105/112).Among them 50 specimens were normal karyotype which accounted for 47.62%(50/105),55 cases were abnormal karyotype accounted for 52.38%(55/105).Abnormal number was the major type of abnormal karyotype.Among them there were 33 cases of trisomy which were most common,6 cases of triploid,1 case of tetraploid and 1 case of compound,2 cases of chimera,1 case of sex-ual chromatids and 6 cases of structural abnormalities.In the specimens with normal karyotype,20 cases were from male,30 cases were from female.Conclusion Embryo chromosome abnormality is one of the factors causing early pregnancy spontaneous abor-tion.Conduct villus chromosome examination in clinical treatment is useful to determine the reasons of abortion and to guide the next pregnancy.

Distribution and Drug Sensitivity of Pathogenic Bacteria among 1513 Cases with Urinary Tract Infection / 中华医院感染学杂志

OBJECTIVE To understand the distribution and the drug resistance of the pathogenic bacteria so as to guide rational application of antibiotics for clinics.METHODS Bacteria culture and drug sensitivity with urine specimen were done among 1513 cases with urinary tract infection.RESULTS Urinary infection was mostly due to Gram-negative bacilli.Gram-positive cocci and fungi were the second.The top four pathogens were Escherichia coli(47.5%),Enterococcus faecium(8.7%),Candida albicans(7.0%),and Klebsiella pneumoniae(6.5%).The positive rates of urinary smear and culture were 51.9% and 29.3%,respectively with a coincidence rate of 77.4%.The drug resistance rates against imipenem,vancomycin and linezolid were zero,with 19.3% and 34.5% against extended-spectrum ?-lactamases-producing E.coli(ESBLs) and K.pneumoniae together,with 33.3% and 58.2% against meticillin-resistant coagulase negative Staphylococcus(MRCNS) and high-level gentamicin resistance(HLGR).CONCLUSIONS Urinary smear,bacteria culture and drug sensitivity tests can offer the basis for clinical rational use of antibiotics.