ABSTRACT
Objective: To To investigate the changes of MicroRNA-134, CREB and p-CREB expression in epileptic rat brains in order to elucidate the molecular mechanisms of epilepsy, providing new ideas for clinical treatment. Methods: Sixty-four Spraque-Dawley (SD) rats were divided into groups randomly, including control group, six hours after seizure group, 24-hour group, three-day group, one-week group, two-week group, four-week group, and eight-week group. All groups were placed under a pilocarpine-induced epilepsy model except the control group, and all rats were decapitated in different points of time. Brain specimens were taken for quantitative PCR experiments, immunohistochemistry and Western blot experiments. The results of the epilepsy model groups and the control group were compared. Results: There were no significant differences between the six hours after seizure group, the 24-hour group and the control group about the MicroRNA-134 levels. MicroRNA-134 in the hippocampus tissue of the three-day group significantly reduced compared with the control group; same result was observed with the one-week, two-week, four-week and eight-week groups. The CREB and p-CREB levels in the three-day group’s rat hippocampus significantly increased compared with the control group; and the high levels of CREB and p-CREB were constantly maintained in the one-week, two-week, four-week and eight-week groups. Conclusions: The MicroRNA-134 level of the epileptic rat hippocampus is significantly lower than normal after three days, and continues to maintain a low level; while CREB and p-CREB levels are rsignificantly increased after three days, and continue to remain at a high level. MicroRNA-134 plays a role in inhibiting synaptic plasticity by inhibiting CREB and p-CREB expressions.
ABSTRACT
Objective To explore the risk factors of earlier neurological function deterioration in acute cerebral infarction patient.Methods 157 cases with acute cerebral infarction had been studied,including the patient's history,general syndromes,clinical characteristic,CT or MRI and laboratory test.Multivarivate noncondition stepwise Logistic model analysis was made after single variable analysis.Results There were 58 patients with neurological function deterioration in 157 cases acute cerebral infarction patient.The morbidity was 36.3%.The following nine factors were associated with earlier neurological function deterioration in acute cerebral infarction i.e. the history of hypertension and diabetes,CT or MRI abnormal,higher WBC count,fever,higher blood sugar,lover mean arterial press,higher fibrin,higher serum Fe-protein;But age,sex,weight,the history of drink and smoker,blood lipemia lever were associated with the disease.Conclusion Earlier neurological function deterioration in acute cerebral infarction patient is caused by multiple factors.Singer test is not easy to calculate the result.To analyze generally patient's clinical material may calculate if earlier neurological function deterioration in acute cerebral infarction patient takes place.