Injectable hydrogel microspheres experimental research for the treatment of osteoarthritis / 中国修复重建外科杂志

OBJECTIVE@#To prepare a novel hyaluronic acid methacrylate (HAMA) hydrogel microspheres loaded polyhedral oligomeric silsesquioxane-diclofenac sodium (POSS-DS) patricles, then investigate its physicochemical characteristics and in vitro and in vivo biological properties.@*METHODS@#Using sulfhydryl POSS (POSS-SH) as a nano-construction platform, polyethylene glycol and DS were chemically linked through the "click chemistry" method to construct functional nanoparticle POSS-DS. The composition was analyzed by nuclear magnetic resonance spectroscopy and the morphology was characterized by transmission electron microscopy. In order to achieve drug sustained release, POSS-DS was encapsulated in HAMA, and hybrid hydrogel microspheres were prepared by microfluidic technology, namely HAMA@POSS-DS. The morphology of the hybrid hydrogel microspheres was characterized by optical microscope and scanning electron microscope. The in vitro degradation and drug release efficiency were observed. Cell counting kit 8 (CCK-8) and live/dead staining were used to detect the effect on chondrocyte proliferation. Moreover, a chondrocyte inflammation model was constructed and cultured with HAMA@POSS-DS. The relevant inflammatory indicators, including collagen type Ⅱ, aggrecan (AGG), matrix metalloproteinase 13 (MMP-13), recombinant A disintegrin and metalloproteinase with thrombospondin 5 (Adamts5), and recombinant tachykinin precursor 1 (TAC1) were detected by immunofluorescence staining and real-time fluorescence quantitative PCR, with normal cultured chondrocytes and the chondrocyte inflammation model without treatment as control group and blank group respectively to further evaluate their anti-inflammatory activity. Finally, by constructing a rat model of knee osteoarthritis, the effectiveness of HAMA@POSS-DS on osteoarthritis was evaluated by X-ray film and Micro-CT examination.@*RESULTS@#The overall particle size of POSS-DS nanoparticles was uniform with a diameter of about 100 nm. HAMA@POSS-DS hydrogel microspheres were opaque spheres with a diameter of about 100 μm and a spherical porous structure. The degradation period was 9 weeks, during which the loaded POSS-DS nanoparticles were slowly released. CCK-8 and live/dead staining showed no obvious cytotoxicity at HAMA@POSS-DS, and POSS-DS released by HAMA@POSS-DS significantly promoted cell proliferation (P<0.05). In the chondrocyte anti-inflammatory experiment, the relative expression of collagen type Ⅱ mRNA in HAMA@POSS-DS group was significantly higher than that in control group and blank group (P<0.05). The relative expression level of AGG mRNA was significantly higher than that of blank group (P<0.05). The relative expressions of MMP-13, Adamts5, and TAC1 mRNA in HAMA@POSS-DS group were significantly lower than those in blank group (P<0.05). In vivo experiments showed that the joint space width decreased after operation in rats with osteoarthritis, but HAMA@POSS-DS delayed the process of joint space narrowing and significantly improved the periarticular osteophytosis (P<0.05).@*CONCLUSION@#HAMA@POSS-DS can effectively regulate the local inflammatory microenvironment and significantly promote chondrocyte proliferation, which is conducive to promoting cartilage regeneration and repair in osteoarthritis.

Investigation of Drinking Water Quality in Rural Areas of Jiangxi Province in 2007 / 环境与健康杂志

Objective To know drinking water quality in the rural areas of Jiangxi province and to provide a scientific basis for making the policy of water improvement.Methods In 2007,twenty-two counties in Jiangxi Province were chosen to investigate the drinking water quality in the rural areas(the types of water source,the ways of water supply,drinking water treatments and population).There were 10-11 monitoring sites in each county.In the dry season(March to May) and wet season(August to October),the water samples were collected,conserved,analyzed and evaluated.Results Of 8 726 568 rural population,16.76% used central water supply taking river water as the water source,83.24%used separate water supply using the shallow and deep layer groundwater as the water source.88.52%of the people consumed the water which was not treated.In wet season and dry season,221 water samples were collected respectively,surface water and groundwater accounted for 21.27%and 78.73% respectively,43.89%and 51.58%of the water samples reached the drinking water standard respectively.In wet season and dry season,the eligible rate of water using surface water as the water source was 63.83%and 59.57%respectively,and it was 38.51% and 49.43%in using groundwater as the water source.In wet season and dry season,the eligible rate of central water supply was 62.79%and 64.84%respectively,it was 31.85%and 42.31%in separate water supply.The total colony count,total coli group,pH, nitrate nitrogen,manganese,iron,arsenic and fluoride were often seen exceeded the related limit.Conclusion The central water supply is not used so widely and microorganism contamination in drinking water is still a main problem in drinking water safety.

Health Investigation on the Use of Faeces in Fishery / 环境与健康杂志

A health investigation on the use of untveat fae-ces as forage in fishery was reported.The colititve in the water and bottom soil of the experimental pool was 10-3,it surpassed the harmless level of faeces,Salmonellesis was not found in the water and bottom soil of experimental pool and the control pool.In the experimental pool,the average COD was 510mg/L,the average BOD5 was 91.15mg/L.being respectively as four times and six times as that of the control pool.The difference between them was significant by means of t-test.In experimental group;the infected rate of clonorchiasis sinensis was 8.11 percent,the medium host was pseudorabora parve,the infected rate of cevcaria was 100 percent,the maxium number of cevcavia per slide was 37.The routine of infection may be relate with the life pattern of the local inhabitant.