ABSTRACT
Objective To investigate the effects of tonsillectomy and adenoidectomy on the body's immune function of preschool children.Methods Using paired comparison method,40 preschool children with OSAHS[aver-age age (4.5 ±1.2)years]were selected.The peripheral venous blood's white blood cells,neutrophils,lymphocytes, monocytes,immune active cells(total T lymphocytes and total B lymphocytes),immunoglobulin(IgA,IgG,IgM)and the content of C3 and C4 levels in the different periods (after 1 month,3 months )before and after tonsillectomy and adenoidectomy were detected.And the immune parameters of 20 healthy children with matched age were selected as control group,the analysis comparison was conducted.Results In preoperative and postoperative 1 month,3 months compared with the control group,the above immune parameters had no statistically significant differences (t =0.332, 0.212,0.336,0.415,0.579,1.295,0.874,1.143,1.296,1.058,1.261,all P >0.05).At postoperative 1 month, the immune active cells(total T lymphocytes and total B lymphocytes),immunoglobulin (IgA,IgG,IgM)and the content of C3 and C4 levels declined slightly compared with preoperation,but within the normal range,back to normal at postoperative 3 months.Conclusion Tonsillectomy and adenoidectomy will not suppress the development of preschool children's immune systems,and the resection is feasible.
ABSTRACT
OBJECTIVE@#To investigate the expression of breast cancer metastasis suppressor 1 (BRMS1) gene protein and the expression of BRMS1 gene promotor area methylation in supraglottic cancer and to evaluate its clinical significance.@*METHOD@#The expression of BRMS1 protein and BRMS1 gene promotor area methylation were examined by using Western blotting method and methylation-specific polymerase chain reaction(MSP) method in 70 cases of supraglottic cancer tissues and 60 cases of their surrounding laryngeal normal mucosa tissues (LNT) and 44 cases of cervical lymph node metastasis of supraglottic cancer.@*RESULT@#Western blot results indicate that BRMS1 protein expression is declined expression level in supraglottic cancer tissue than the expression of BRMS1 protein in LNT of supraglottic cancer. Compared with para carcinoma normal laryngeal mucous tissue, BRMS1 gene protein in supraglottic cancer tissue primary lesion decreased obviously, and it is decreased more obviously in cervical lymph node metastasis lesion, the discrepancy is notable (P < 0.05). MSP results indicate BRMS1 gene promotor methylation is coordinated with its down-expression in supraglottic cancer tissue. BRMS1 promotor area methylation analysis reveal that there were 34 patients with methylation in 70 patients' supraglottic cancer tumor primary lesion, hold 48.6% (34/70); 32 patients have methylation in 44 patients' cervical metastasis lymph node tissue, hold 72.7% (32/44); however, there is no methylation in 60 para carcinoma tissue (r(s) = 0.66, P < 0.05).@*CONCLUSION@#The expression of BRMS1 protein in supraglottic cancer is significantly decreased. It had correlation with clinical stage and pathologic differentiation and cervical lymph node metastasis of supraglottic cancer. BRMS1 gene promotor methylation is related with down-expression of BRMS1 gene protein of supraglottic cancer. Maybe BRMS1 gene promotor methylation is one of the reasons of its down-expression.
Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Carcinoma, Squamous Cell , Metabolism , Pathology , DNA Methylation , Glottis , Head and Neck Neoplasms , Metabolism , Pathology , Laryngeal Neoplasms , Metabolism , Pathology , Lymph Nodes , Pathology , Lymphatic Metastasis , Neoplasm Proteins , Metabolism , Neoplasm Staging , Promoter Regions, Genetic , RNA, Messenger , Genetics , Repressor Proteins , Squamous Cell Carcinoma of Head and NeckABSTRACT
Objective:To determine clinical significance and expression of cellular proliferative antigen Ki-67 in laryngeal carcinoma tissues.Methods:Ki-67 expressions in 41 cases of laryngeal carcinoma tissues were detected by immunohistochemical S-P method;12 cases of pericarcinoma tissues were used as control and comparison.Results:The positive expression rate of Ki-67 was 53.6% in 41 cases of laryngeal carcinoma tissues and 16.7% in 12 cases of pericarcinoma tissues,which showed significant difference ( P