ABSTRACT
OBJECTIVE@#To seek a precise method for estimating postmortem interval (PMI) in forensic medicine practice.@*METHODS@#This study detected the concentration of 21 elements in the vitreous humor of rabbit within 96 hours after death and explored the relationship between their concentration and PMI using the method of ICP-MS.@*RESULT@#It was shown that the concentration of 9 elements in vitreous humor of rabbit within different intervals after death were correlated to PMI available as a reference indicator to estimate PMI.@*CONCLUSION@#The changes in the concentration of certain elements in vitreous humor of vitreous humor are correlated with PMI and determination of such changes can be used to estimate the time of death.
Subject(s)
Animals , Rabbits , Calcium/analysis , Forensic Medicine , Iron/analysis , Magnesium/analysis , Postmortem Changes , Potassium/analysis , Time Factors , Vitreous Body/chemistryABSTRACT
OBJECTIVE@#To seek a exact method of estimating postmortem interval (PMI).@*METHODS@#This study detected the concentration of magnesium (Mg) and iron (Fe) in vitreous humor of rabbit at 96 h after death and explored the relationship between their concentration and PMI using a method ICP-MS.@*RESULTS@#The concentra-tion of Mg in vitreous humor of rabbit at 48 h after death and Fe in vitreous humor of rabbit at 6-48 h after death were related to PMI significantly. The formulae of the relationship between PMI and Mg concentrations is y = 0.0738x2 + 0.6997x + 11.45 (within 48 h, R2 = 0.9119). The formulae of the relationship between PMI and Fe concentrations is y = 0.0411x2 - 0.3148x + 1.4113 (within 6-48 h, R2 = 0.9594).@*CONCLUSION@#The concentration of Mg in vitreous humor of rabbit at 48 h after death and Fe in vitreous humor of rabbit at 6-48 h after death may be as reference indicator to estimate PMI.
Subject(s)
Animals , Rabbits , Iron/analysis , Magnesium/analysis , Mass Spectrometry/methods , Postmortem Changes , Time Factors , Vitreous Body/chemistryABSTRACT
OBJECTIVE@#To seek a exact method of estimating postmortem interval (PMI).@*METHODS@#This study detected the concentration of zincum(Zn) and nickel(Ni) in vitreous humor of rabbit at hour 96 after death and explored the relationship between their concentration and PMI using a method ICP-MS.@*RESULTS@#The concentration of Zn and Ni in vitreous humor of rabbit at hour 24 after death were related to PMI significantly; The formulae of the relationship between PMI and Zn concentrations is y = 0.1404x2 - 1.3351x + 3.8298 (within 24 h; R2 = 0.9202). The formula of the relationship between PMI and Ni concentrations is y = 0.0043x2 - 0.0596x + 0.2665(within 24 h; R2 = 0.9103).@*CONCLUSION@#The concentration of Zn and Ni in vitreous humor of rabbit may be a reference indicator to estimate early PMI.
Subject(s)
Animals , Female , Male , Rabbits , Forensic Medicine , Nickel/analysis , Postmortem Changes , Time Factors , Vitreous Body/chemistry , Zinc/analysisABSTRACT
OBJECTIVE@#To seek a exact method of estimating Postmortem interval (PMI).@*METHODS@#This study was preformed to investigate the relationship between postmortem interval and absorbance in vitreous humor of rabbit after death. The absorbance in vitreous humor of 48 rabbits after death were investigated with Model 754 spectrophotometer in apt wavelength (420 nm).@*RESULTS@#There exists positive linear regression association between postmortem interval (Y) and absorbance in vitreous humor (X) (r = 0.98327, P < 0.05), during rabbits after death 0 to 72 hours. The formula of linear regression is Y = 453.30 X + 0.75 (Y = postmortem interval = PMI, X = absorbance in vitreous humor).@*CONCLUSION@#The absorbance in vitreous humor can be as reference indicator to estimate PMI within hour 72.