Curcumin influence the expression of matrix metalloproteinases in pterygium epithelial via inhibiting NF-κB signaling pathway / 医学研究生学报

Objective Ultraviolet radiation can induce the expression of matrix metalloproteinases (MMPs) in pterygium epithelial cells. To investigate the effects of curcumin on the expression of MMP 2 and MMP 9 in human pterygium fibroblasts (HPFs) on UVA-irradiation and its possible mechanism.Methods After optimizing the dose of UVA irradiation and the concentration of curcumin, HPFs in the second generation were divided into control group (no exposure to UVA, no medication), UVA group (exposure to UVA), and UVA + curcumin group (exposure to UVA + curcumin). MMP-2 and MMP-9 levels were tested by zymography. The mRNA expression of MMP-2 and MMP-9 was detected by RT-PCR. The NF-κB-DNA binding activity was detected by electrophoretic mobility shift assay (EMSA).Results Compared with the control group, the secretion of MMP-2 and MMP-9 in the UVA group was increased significantly (P<0.05), which could be suppressed by curcumin (P<0.05). The MMP-9 mRNA levels were also significantly increased in UVA group \[(100±0)% vs (247.0±10.8)%, P<0.05\], and could be inhibited by curcumin \[(88.7±5.1)% vs (247.0±10.8)%, P<0.05\]. The NF-κB-DNA binding was remarkably increased in UVA group (P<0.05), and significantly decreased after treatment with curcumin (P<0.05).Conclusion Curcumin can inhibit the expression of MMP-2 and MMP-9 in UVA-irradiated HPFs, and the mechanism involved in this protective effect might be its inhibitory effect on NF-κB activation.

Systemic lupus erythematosus affects the tear film and objective visual quality of the patient / 医学研究生学报

Objective Systemic lupus erythematosus (SLE) cause irreversible damage to multiple organs of the body.This article evaluates the changes in tear film quality and visual quality in patients with SLE.Methods In the study,we selected 66 patients with SLE (66 right eyes) for the study group and 59 healthy volunteers (59 right eyes) for the control group,The measurements of BUT,corneal fluorescein staining(FL) score and SIt were taken in turn for both two groups.Anterior segment OCT was used for measuring the CET,CCT,TMD,TMH and TMA.OQAS Ⅱ system was taken for MTFcutoff,SR,static OSI,OV (100％,20％,9％) and dynamic OSI.Differences of the parameters were compared between the two groups.The data correlation was assessed by Spearman rank correlation analysis.Results There were significant differences in SIt,BUT,FL score,CET,TMH,TMD and TMA between the two groups(P＜0.05),but no significant difference in CCT (P＞0.05).The differences of static OSI,MTFcutoff,SR,OV100％,OV20％,OV9％ between the two groups were statistically significant(P＜0.01).The △OSI(differences between dynamic and static OSI) had the difference between the two groups(t=4.615,P＜0.001).TMA was positively correlated with CET,SIt,and BUT (P＜0.05),and had no correlation with OSI,MTFcutoff,SR,OV100％,OV20％,OV9％ (P＞0.05).Anti-ds-DNA is positively correlated with SIt,TMA,MTFcutoff and SR (r =0.346,0.208,0.257,0.197,P＜0.05) and negatively correlated with OSI(r=-0.292,P＜0.05).There was no correlation between ANA,C3,C4 and the parameters of tear film and visual quality (P＞0.05).Conclusion SLE may affect the amount and stability of the patient's tears and affects the corneal epithelial thickness and visual quality.

Identification of the related substances in fasudil hydrochloride with hyphenated techniques / 药学学报

The study aims to identify the related substances in fasudil hydrochloride by hyphenated techniques. A WondaSil C18 (250 mm x 4.6 mm, 5 microm) column was used for the separation of the related substances with a mixture of methanol and ammonium acetate buffer solution as the mobile phase by gradient elution. The structures of the related substances were speculated by electrospray positive ionization LC-TOF/MS accurate ion mass and MS/MS determination and elucidation, and verified further through synthesis and spectroscopic analysis. Fasudil hydrochloride and the related substances were separated under the established HPLC condition. Three related substances in fasudil hydrochloride were characterized by hyphenated techniques. The hyphenated LC-MS method is useful for the identification of related substances in fasudil hydrochloride and the results obtained are valuable for its manufacturing process and quality control.

CT and MRI diagnosis of congenital stenosis of the internal auditory canal / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To investigate multiple slice computed tomography (MSCT) and magnetic resonance imaging (MRI) features of congenital stenosis of the internal auditory canal (CSIAC) and improve the ability for diagnosis.</p><p><b>METHOD</b>Thirteen cases with fifteen ears were studied. In all cases a MSCT and MRI was performed.</p><p><b>RESULTS</b>Eleven cases were unilateral, and 2 cases were bilateral. MSCT could show the narrowness of IAC. Three cases were isolated, but the others were combined with inner ear malformations. One ear had inner, middle and outer ear malformations. One ear had inner, middle, and outer ear malformations with a frontal bone malformation. MRI demonstrated that all of the vestibulocochlear nerves were hypoplastic. The cochlear nerve in seven ears was not present, in seven ears the nerve was thinner, and in the last case it was poorly visualized. The facial nerve in two ears was hypoplastic. Volume rendering (VR) could present the degree of the narrowed internal auditory canals, combined with other inner ear anomalies.</p><p><b>CONCLUSION</b>MSCT will show the degree of the narrow internal auditory canals and combined anomalies, while the MRI can further demonstrate the nerves' development.</p>

Imaging features of duplication of the internal auditory canal / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To explore multiple slices computed tomography (MSCT) and magnetic resonance imaging (MRI) features of duplication of the internal auditory canal (DIAC) in order to improve the accuracy of diagnosis.</p><p><b>METHODS</b>Four cases (5 ears) were analyzed and the related documents were reviewed retrospectively. MSCT was performed on all cases, and two cases had MRI scanning at the same time.</p><p><b>RESULTS</b>MSCT has shown that the internal auditory canal were divided into two canals by a bony septum in 5 ears. The superior canal ended in a very narrow connection to the facial canal, the inferior portion ended in connection to the cochlea and vestibule. The bony septums from the 2 ears were found no longer intact. The sum of diameter of the two canals was greater than 2 mm. In addition, 5 ears were found to have an enlarged vestibules and the hypoplasia lateral semicircular canals, and meanwhile, 2 ears of them were combined with ipsilateral microtia. Also 1 case of them was combined with microtia, outer acoustic atresia as well as abnormal middle ear. Multiplanar reconstruction and volume rendering images can entirely show the bony septum and two canals. In this study, the vestibular nerve, cochlear nerve and facial nerve were total hypoplastic in one ear, in the other ear, the vestibular and cochlear nerve were hypoplastic, and however, the facial nerve was intact.</p><p><b>CONCLUSIONS</b>MSCT can clearly depict duplication of the internal auditory canals and concomitant anomalies. MRI can clearly show the neural components and their associated malformation.</p>