ABSTRACT
Currently,antimicrobial resistance has become an urgent global public health problem.Human defensin,a family of innate immune peptides,plays an important role in the control of infectious disease because of its low resistance to microbiological infection.However,so far there is seldom report on industrial production of bioactive defensin.Human β-defensin 3 (HBD3) is a member of human innate antimicrobial peptides.It has broad-spectrum antimicrobial activity and function in a variety of inflammation diseases.In addition,HBD3 is considered as a good candidate for antibiotics,because it has a strong killing activity to antibiotics-resistant microbes and resistance to highsalt environment,it is especially valuable for the treatment of ocular surface inflammatory diseases.This review covers the in vitro research on the production of HBD3,optimization of structure-activity and its antimicrobial potential for ocular surface infection,which may provide a supportive evidence for the commercial production of HBD3 and other antimicrobial peptides in biopharmaceutical area.
ABSTRACT
OBJECTIVE@#To solve the difficulties of identification of Sarcosaphagous flies such as Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) which could not be identified by analyzing the 278bp and 635 bp regions of the gene encoding for cytochrome oxidase subunit I and II (CO I and CO II) in mtDNA.@*METHODS@#Specimens were collected from the corpses of rabbits on the grassland in Huhhot and Chengdu, the sequences of 551 bp region of 16S rDNA of their mtDNA were analyzed, the multiple-alignment program DNAMAN(version 4.0) and MEGA 2.1 sofeware were employed for sequence alignments neighbour-joining tree construction.@*RESULTS@#Lucilia sericata (Meigen) and Lucilia cuprina (Wiedemann) were distinguished successfully by sequence analysis of The 551 bp region of the gene of 16S rDNA.@*CONCLUSION@#The 551 bp region of the gene of 16S rDNA of sarcosaphagous flies can be used for identifying them on species level effectively. It is likely to be a successful compliment to identify the sarcosaphagous flies by sequence analysis of CO I and CO II in mtDNA.