ABSTRACT
Objective To investigate the application prospective of carboxyfullerene C_3 as a radioprotectant or assistant for tumor radiotherapy.Methods Different concentrations of C_3 were incubated with K562 and AHH-1 cell,CCK-8 assay and trypan blue rejection test were performed to examine the influence of C_3 on the cell viability.Annexin V/PI staining and flow cytometry assay were applied to assess the cell cycle and apoptosis after 7-ray irradiation.Results C_3 showed little toxicity to AHH-1 cell with the survival rate over 95% ,but 600 mg/L of C_3 markedly inhibited the growth of K562 cell (82%) .Pretreatment of 100 mg/L C_3 significantly increased the survival rate of AHH-1 cell after 4 Gy irradiation compared with the single radiation group(71.3% vs 90.3%) ,but decreased the apoptosis rate (26.3% vs 12.6%) ,while the survival rate of K562 cell was decreased and the apoptosis rate was elevated with the increase of C_3 concentration.Moreover,the cell cycle analysis revealed the G_2 phase block in AHH-1 cell after radiation exposure was mitigated by C_3 pretreatment,but that in K562 cell was aggravated.Conclusions C_3 has good radioprotective effects on AHH-1 cells.For K562 cell,C_3 could inhibit the cell proliferation,promote the radiation induced apoptosis and aggravate the G_2 phase block.
ABSTRACT
Objective:To investigate the effect of simvastatin on the expression of vascular endothelial growth factor (VEGF)and its receptor in cultured human umbilical vein endothelial cells(HUVEC).Methods:Cells were randomly divided into simvastatin group,tumor necrosis factor-?(TNF-?)group,simvastatin+TNF-?group,interleukin-1?(IL-1?)group,simvastatin+IL-1?group and control group.The effects of TNF-?,IL-1?and simvastatin on the protein levels of VEGF and its receptor in HUVEC were studied by immunocytochemistry.Results:The protein levels of VEGF and its receptor in TNF- ?group and IL-1?group were significantly higher than those of control group,and simvastatin decreased the protein levels of VEGF and its receptor in TNF-?group and IL-1?group.Conclusion:Simvastatin can decrease the protein levels of VEGF and its receptor in HUVEC. [