ABSTRACT
Medical cell biology is an important basic medical course. Owing to its complex and esoteric knowledge and few lessons, there is a dilemma of teaching and learning. From learning situation analysis, the course team building programs, operation and management of the program, learning evaluation program, the author has conducted exploration and preliminarily proved that the medical cell biology curriculum team learning is conducive to the cultivation of students ' innovation ability and the team cooperation spirit as well as improvement of students ' learning interest, which is worthy of application and promotion.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the absorption enhancen effect of borneol/mentholum eutectic mixture (BO/ME) on nasal-brain delivery of neurotoxin loaded nanoparticles.</p><p><b>METHOD</b>Using microdialysis sampling technique in awake freely-moving rats, the counter per minute (cpm) of dialysates in right PAG of NT-loaded nanoparticles with the BO/ME (BO/ME-NT-NP), radiolabeled with sodium 125I-Iodide, were measured in a gamma-counter for radioactivity. After converting cpm into corresponding concentrations of NT byin vivorecovery of microdialysis probes, the pharmacokinetic parameters were calculated.</p><p><b>RESULT</b>The BO/ME-NT-NP could be absorbed into the brain, much better to NT-NP and the nanoparticles with borneol or menthdlum only, and the pharmacokinetics accorded with the two-compartment model. The parameters tmax, cmax, AUC, t 1/2(beta) were 0.68 h, 27.32 ng x mL(-1), 132.68 ng x h x mL(-1), 3.1076 h.</p><p><b>CONCLUSION</b>With adding BO/ME as absorption enhancer, NT could be significantly increased in the brain with the help of nanopartilces as carriers, and the time to maximal concentration was short, the elimination process was prolonged.</p>