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AIM: To investigate the association of osteoprotegerin ( OPG) gene single nucleotide polymor-phisms (SNPs), 163A/G (rs3102735) and 245T/G (rs3134069), with susceptibility to rheumatoid arthritis (RA) in Chinese Han population .METHODS:A total of 205 patients with RA and 171 healthy control subjects were enrolled into this study.Genotyping was performed by polymerase chain reaction-based restriction fragment length polymorphism and subsequently confirmed by DNA sequencing .Odds ratio ( OR) and 95%confidence intervals ( CI) were calculated for the risk genotypes and alleles .RESULTS: OPG gene polymorphisms 163A/G and 245T/G were conformed to the Hardy-Weinberg equilibrium .The statistical differences in the genotypes of AA , AG and GG at 163A/G locus were found in RA and controls.The G allele was associated with an increased risk of RA , with OR of 1.219 (95%CI:1.066~2.339).No significant difference was observed between RA group and control group with respect to genotypic and allelic frequencies of OPG gene 245T/G (P>0.05).CONCLUSION:The OPG gene 163A/G SNP may be associated with RA susceptibility , and G allele may be the risk factor for developing RA .
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Objective To investigate the expression of connective tissue growth factor(CTGF),coll agen I and collagen Ⅲ in sacroiliac joint(SIJ)of patients with spondyloarthropathy(SpA).Methods Thirty patients with SpA,including 17 patients with grade Ⅱ saeroiliitis and 13 patients with grade Ⅰ sacroiliitis,were performed on CT guided needie biopsy of SIJ.After sacroiliitis were confirmed by staining with hematoxylin and eosin in sacroiliac joint tissue sample,immunohistochemical assay was performed to determine the expression of CTGF,collagen Ⅰ and collagen Ⅲ in sacroiliac ioint tissue.Univariate Chi-square test was used for data comparison between multiple groups and t-test was used for two group data comparison.Results Contrast to healthy controls,CTGF were found upexpressed on the cytoplasm of inflammatory cells in pannus and bone marrow of sacroiliac tissue samples of patients with SpA,while collagen I and collagen Ⅲ were found up-expressed in bone,cartilage and ligament tissue[(57.9±42.4)/HP vs(2.7±2.5)/HP P<0.05,0.298±0.080 vs 0.044±0.024 and 28.254±41.165 vs 0.105±0.054.P<0.05 respectively].Conclusion CTGF,collagen Ⅰ and collagen Ⅲ are up-expressed in SIJ of SpA patients.CTGF may play an important role in articular cartilage fibrosis and ossification of SpA.
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Objective To determine the allele and genotype frequencies of two single nucleotide polymor-phisms (SNPs) 677C/T,1298A/C in the methylenetetrahydrofolate reductase(MTHFR) gene in Chinese Southern Han individuals, and to assess whether there are differences in alleles frequencies among rheumatoid arthritis(RA) patients and normal controls. Methods 187 subjects(101 normal controls and 86 RA patients) were analyzed. The slide frequencies were carried out with polymerase chain reaction-restriction fragment langth polymorphism (PCR-RFLP). The two MTHFR SNPs Hardy-Weinberg equilibrium, linkage disequilibrium were analyzed with population genetics methods. Results The allele distribution of SNPs was in good unity with Hardy-Weinberg eqilibrium. The genotype frequencies of 677CC, CT, TT were 36.0%, 51.2%, 12.8%, respectively, in RA group, and 42.6%, 44.6% ,12.9%, respectively, in controls. The genotype frequencies of 1298AA,AC、CC were 60.5%、32.6%、 7.0%, respectively, in RA group, and 49.5% ,46.5% ,4.0% ,respectively, in controls. The frequencies of rarely allele 677T were 38. 4% and 35.1% in RA and controls. The 1298C were 23.3% and 27.2% in RA and controls. Allele frequencies were similar between RA group and controls, and there is no linkage between two SNPs and RA. The relationship between 677C/T and 1298A/C was strong linkage disequilibrium. Conclusions The research pro-vided Chinese southern Han population genetics data on MTHFR gene. The relationship between 677C/T and 1298A/C was strong linkage disequilibrium.