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Objective To improve the sensitivity of detection of protein P16 by FCM.Methods Protein P16 of blood cells and tumor cells in biopsy samples were labeled simultaneously with anti-CD45 and anti-P16 fluorescent antibody.The rates of CD45+P16+ cells and CD45-P16+ cells were calculated by FCM.Results The low expression rates of protein P16 in normal blood cells and in tumor cells in biopsy samples were 15.39%(6/39) and 69.23%(27/39),respectively, which had a significant differences (P
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Objective:To study the influence of surgical operation on cellular kinetics in peripheral blood monocytes of patients with malignant tumors.Methods:Apoptosis level and proliferative activity were measured and compared between before and after surgical opration in peripheral blood monocytes of patients with malignant tumors.Rusults:Apoptosis level of patients with malignant tumors after surgical operation was lower than that of patients before surgical operation( P
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Objective To study the causes of mis diagnosis and the experience of diagnosis and management for cholelithiasis complicated with abdominal tumors.Methods Clinical data of 36 cases of cholelithiasis complicated with abdominal tumors were analyzed retrospectively.Among them,24 were male,12 female.The age ranged from 48 to 82 year old.Each case was diagnosis as cholelithiasis and admitted to our hospital.Results In 36 patients,18 was diagnosed as complecated with abdominal tumors preoperatively;12 was diagnosed intraoperatively,while in 6 cases the tumor was misdiagmosed for 5 days to 3 months,finally the tumor was comfirmed by reoperation and pathology.Among the 36 cases,the tumors cluding 5 of carcinoma of gallbladder(13.9%),4 of cholangiocarcinoma(11.1%),3 of hepatoma(8.3%),6 of pancreatic carcinoma(16.7%),6 of gastric carcinoma(16.7%),7 of colon carcinoma(19.4%),2 of rectal carcinoma(5.5%).Of them,2 cases refused operation,2 cases underwent intervention operation,the others reseived operation.During primary operation,radical resection of the tumor and cholecystectomy and/or common bile duct(CBD) exploration was performed in 18 patients,palliation resection and cholecystectomy and/or CBD exploration performed in 4 patients,exploratory laparotomy performed in 4 patients,and only LC and/or CBD exploration performed in 6 patients.Of the latters,reoperation was performed 5 days to 3 months after primary operation.4 patients had tumor radical resection,1 had palliation resection,and 1 had interventional therapy.Conclusions Cholelithiasis may complicated with abdominal tumor,especially with digestive tract tumor.For cholelithiasis patient the history-taking need to be done carefully before operation.For the senile patient and the patient without typical symptoms or physical signs,the systems checking shoud be done carefully preoperatively,and abdominal cavity should be checked carefully intraoperatvely to find the tumor,which may exist in abdominal cavity.the patient with syndrome of post-cholecystectomy shoud be carefully followed up to avoid the mis diagnosis and mistreatment of the tumor.
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Objective: To study tumor chemotherapy on IL-2R express in peripheral blood cells of patients with malignant tumors.Methods: CD + 25 cells percentage rate of peripheral blood cells in patients with or without chemotherapy was done.Results: CD + 25 cells percentage rate in malignant tumor was lower than those of normal contrlos (P
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Objective To investigate the effect of radiotherapy on cellular kinetics of peripheral blood monocytes in patients with malignant tumors. Methods In patients with malignant tumors, apoptosis and proliferative activity of peripheral blood monocytes were monitored and compared before, during and after radiotherapy. Results Apoptosis and S phase fraction in cancer patients on radiotherapy were higher as compared to these without radiotherapy or the normal control group (8.93%?6.94%, 0.20%?0.20%, 0.15% ?0.13%, and 1.23%?1.15%, 0.54%?0.46%, and 0.33%?0.26%,respectively,F=5.77, P