ABSTRACT
<p><b>OBJECTIVE</b>To explore the clinicopathologic features of CD30-positive sinusoidal large B-cell lymphoma (CD30 + SLBCL) and its relative correlation with Epstein-Barr virus (EBV).</p><p><b>METHODS</b>Two cases of CD30 + SLBCL, a 65-year-old men and a 85-year-old women were morphologically and immunophenotypically analyzed. EBV status was also evaluated through not only the polymerase chain reaction (PCR) amplification to the EBV Bam HIW DNA sequence, but also an immunohistochemical detection of the latent membrane protein 1 (LMP1).</p><p><b>RESULTS</b>The patients presented with similarly superficial lymphadenopathy. One of them died of the tumor within 10 months. Microscopically, both of the neoplasms were characterized by a cohesive sinus growth pattern and the monomorphic cytology of the tumor cells. Immunohistochemically, They were both positive for CD45, CD30, and CD20 or CD79alpha, whereas neither expressed EMA, ALK1, nor any histiocytic/T-lineage markers. No evidence of EBV-infection could be found either.</p><p><b>CONCLUSIONS</b>CD30 + SLBCL is a morphologically and immunophenotypically distinctive variant of diffuse large B-cell lymphoma, which should be distinguished from T/null cell type anaplastic large cell lymphoma and some other nodal lesions with a predominantly sinusoidal infiltrative pattern. CD30 + SLBCL may not be correlation with EBV.</p>
Subject(s)
Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Diagnosis, Differential , Immunohistochemistry , Ki-1 Antigen , Lymphoma, B-Cell , Diagnosis , Pathology , Lymphoma, Large-Cell, Anaplastic , Diagnosis , PathologyABSTRACT
Purpose:To investigate the existence of micro sa tellite instability in nodal non-Hodgkin's lymphomas by microsatellite analysis of BAT-26 and BAT-25.Methods:Frozen tissues of 51 nodal non-Hodgkin's lymphomas and 9 lymphoid reactive hyperplasia were collected by surgical biopsy. Genomic DNA was extracted. Microsatellite alterations of BAT-26 and BAT-25 were detected b y polymerase chain reaction(PCR)followed by fragment analysis using automatic DNA sequencer and GeneScan 3.1 software. 10 cases of hereditary nonpolyposis col orectal cancers with known high frequency microsatellite instability (MSI-H) we re retrieved as positive controls. Results:No aberration of mon onucleotide duplication in the microsatellite markers BAT-26 and BAT-25 was ob served in all successfully amplified specimens.Conclusions:The microsatellite analysis of BAT-26 and BAT-25, two highly sensitive markers to microsatellite status in colorectal tumors, demonstrated no evidence of MSI-H i n nodal non-Hodgkin's lymphomas. The existence of low frequency microsatellite instability and participation of mismatch repair genes in lymphomagenesis remain to be determined by further studies.