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Objective To investigate the therapeutic effect of integrated traditional Chinese and western medicine for acute severe traumatic brain injury.Methods The patients with acute severe traumatic brain injury were randomized into the treatment group(receiving integrated traditional Chinese and western medicine),control 1 group(receiving routine western medicine and blood-activating and stasis-removing herbs) and control 2 group(receiving routine western medicine).The therapeutic effect,neurological impairment score and complications were compared in the three groups after treatment for 3 weeks.Results In the treatment group,13 patients were markedly effective,5 effective,2 ineffective and the total effective rate was 90.0%;in control 1 group,8 patients were markedly effective,4 effective,8 ineffective and the total effective rate was 60.0%;in control 2 group,9 patients were markedly effective,6 effective,5 ineffective and the total effective rate was 75.0%.The therapeutic effect in the treatment group was superior to that in the two control groups(P
ABSTRACT
Objective To establish a method for the in-vitro culture of astrocytes from cerebral cortex of neonatal rats.Methods Astrocytes were isolated from cerebral cortex of the neonatal SD rats aged 24 hours,and then were cultured in the high-glucose DMEM medium with 10% fetal bovine serum added.The morphological features of astrocytes were examined under inverted microscope.The growth of the third generation of astrocytes was observed with counting plate method and methyl thiazolyl tetrazolium (MTT) assay.Results The astrocytes from cerebral cortex of neonatal rats grew well,the astrocytes showed typical morphological features,and no other kinds of cells was found.Conclusion A simple and practical in-vitro culture method for rats astrocytes is established.