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Background: The extracapsular involvement (ECI) of lymph node metastasis has been considered as an important prognostic factor in patients with malignancies. Aims: To investigate the influence of ECI of lymph node metastasis on prognosis of patients with gastric cancer and its correlation with expression of transcriptional factor SOX9. Methods: A retrospective study was conducted in 200 consecutive gastric cancer patients with lymph node metastasis (TNM stage Ⅰ-Ⅲ) from Jan. 2010 to Dec. 2014 at Xi'an No. 1 Hospital. Patients were allocated into two groups according to the presence or absence of ECI, and their differences in clinicopathological characteristics, overall survival and SOX9 expression were compared. Univariate and multivariate analyses were performed by Cox proportional hazard model to identify the risk factors for poor prognosis. Results: ECI was associated with advanced T stage (T3, T4), N stage (N2, N3) and TNM stage (stage Ⅱ-Ⅲ), respectively (P<0.05). Furthermore, high expression of SOX9 was more frequently seen in metastatic lymph node with ECI than that without (94.5% vs. 58.3%, P<0.05). Kaplan-Meier survival analysis confirmed that patients in ECI group were associated with significantly shorter overall survival time (P<0.05). In multivariate analysis, TNM stage Ⅱ-Ⅲ (HR=3.224, 95% CI: 2.769-5.283, P<0.001), ECI of lymph node metastasis (HR=2.388, 95% CI: 1.802-3.209, P<0.001) and high expression of SOX9 in metastatic lymph node (HR=1.321, 95% CI: 1.201-1.684, P=0.001) were found to be the independent risk factors of poor overall survival in patients with gastric cancer. Conclusions: ECI of lymph node metastasis is associated with high expression of SOX9 in gastric cancer patients and can be used as an independent risk factor for poor prognosis. Also, ECI is a histomorphological indicator for invasive and metastatic phenotype of gastric cancer.
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Background:Angiogenesis plays a critical role in tumor growth and metastasis. AGGF1,a new member of angiogenic factors,has been shown to be aberrantly expressed in a variety of malignant tumors. Aims:To investigate the correlation of AGGF1 expression with prognosis of patients with colon cancer. Methods:Eighty colon cancer patients undergoing surgical operation from Jan. 2010 to Dec. 2012 at Xi’an First Hospital were recruited. Immunohistochemistry was performed to evaluate the expression of AGGF1 in cancerous and paired paracancerous tissues. The correlations of AGGF1 expression in cancerous tissue with clinicopathological characteristics and prognosis were analyzed. Cox regression model was used to identify the prognostic factors of colon cancer. Results:AGGF1 expression was significantly higher in colon cancer tissue than in paracancerous tissue (67. 5% vs. 32. 5%,P＜0. 05),and was correlated with tumor differentiation,lymph node metastasis,vascular/lymphatic infiltration and TNM stage (P＜0. 05). Kaplan-Meier survival analysis showed that high AGGF1 expression was correlated with decrease of survival rate in colon cancer patients (P ＜0. 05). Univariate and multivariate analyses identified AGGF1 as a prognostic factor of patients with colon cancer (OR＝2. 09,95% CI:1. 67-4. 45,P＝0. 011;OR ＝1. 98,95% CI:1. 72-4. 59,P ＝0. 000 ). Conclusions:Angiogenic factor AGGF1 is up-regulated and correlated with tumor metastasis and unfavorable prognosis in patients with colon cancer. It might be a potential prognostic indicator and deserves further study.
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Objective To investigate the Relationship between the CYP2C19 polymorphism and the efficacy of triple therapy with lansoprazole on Helicobacter pylori infection. Methods 125 elderly patients diagnosed with H.pylori infection were treated with triple therapy. Polymorphism of CYP2C19 was measured by AS-PCR. 105 young patients were selected as control group. The relationship between the polymorphism and eradication rate were analyzed. Results Among the 125 patients,eradication rate of extensive metabolizer group,internal metabolizer group and poor metabolizer groups was 85.29%,76%and 89.39%, respectively. There was no significant difference between groups (P > 0.05). Eradication rate showed no difference between experimental and control group either (P>0.05)(P>0.05). Conclusion The results suggests that the CYP2C19 polymorphism has no correlation with the eradication rate of Helicobacter pylori infection by triple therapy with lansoprazole in elderly patients.