ABSTRACT
<p><b>OBJECTIVE</b>To examine and analyze the interleukin (IL)-1B gene single nucleotide polymorphism (SNP) at positions +3954, and explore the association between SNP and risk of chronic periodontitis (CP) among the Hui and Dongxiang minorities in Gansu province.</p><p><b>METHODS</b>CP group consisted of 215 patients from Hui and Dongxiang and healthy control group consisted of 219 subjects. Anti-coagulated peripheral blood sample was obtained from each subject and genomic DNA was extracted from each sample. SNP at IL-1B+3954 were analyzed by standard polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.</p><p><b>RESULTS</b>Statistically significant differences were found in genotype and allele frequencies between CP group and healthy control group. The exist of position alleles C increased the incidence of CP. The trend of frequency distribution of gene polymorphism above in Dongxiang was the same as that of Hui.</p><p><b>CONCLUSION</b>The present study reveals that IL-1B+3954C/T genotypes are significantly associated with the risk of CP. These results indicate that genetic polymorphism is significantly associated with the risk of CP among the Hui and Dongxiang minorities in Gansu province.</p>
Subject(s)
Adult , Female , Humans , Male , Middle Aged , Alleles , Chronic Periodontitis , Gene Frequency , Genetic Predisposition to Disease , Genotype , Interleukin-1 , Polymerase Chain Reaction , Polymorphism, Genetic , Polymorphism, Restriction Fragment Length , Polymorphism, Single NucleotideABSTRACT
5 000 respectively. Results Among 2 175 cases with clonorchis eggs, 1 244 cases showed clinical complications (57.2%). The average EPG in 61 cases counted was 3 055, 34 (55.7%) showed medium or heavy intensity of infection. The cases distributed in 80% of the townships, more concentrated in 2 towns and 1 farm. The patients aged between 3 to 76 years old with a ratio of male to female 3.6 ∶ 1. 45.5% of the cases were farmers. 1 308 cases (60.1%) were Zhuang Minority. The number of cases seeking medical treatment increases year by year since mid-1980s. 66.0% of the cases had a habit of raw fish-eating. Conclusion Clonorchiasis sinensis widely distributes in Guigang City, number of cases admitted in hospitals increases yearly.