ABSTRACT
Objective :To study influence of valsartan combined hydrochlorothiazide on 24h diastolic blood pressure (DBP) ,24h systolic blood pressure (SBP) and blood pressure variability (BPV) in patients with hypertension .Meth—ods : A total of 94 hypertensive patients treated in our hospital were randomly and equally divided into valsartan group and combined treatment group (received valsartan combined hydrochlorothiazide ) ,both groups were treated for one month .The 24hDBP ,24hSBP ,BPV ,levels of nitric oxide (NO ) and endothelin (ET ) before and after treatment and incidence of adverse reactions were compared between two groups .Results : Compared with valsartan group after treatment ,there were significant reductions in 24hSBP [ (130. 64 ± 10.01) mmHg vs .(121.53 ± 9.35) mmHg] ,24h DBP variability (24hDBPV) [ (12.47 ± 3.32)% vs.(10.82 ± 2.71)%] ,24hSBPV [ (10. 42 ± 2. 00)%vs.(8.51 ± 1. 64)%] and daytime SBPV [ (10.87 ± 2. 05)% vs.(8. 66 ± 1.65)%] , P＜0. 05 or ＜0.01 ;significant rise in NO level [ (42.92 ± 6.84) μmol／L vs.(53.43 ± 7. 16) μmol／L] and significant reduction in ET level [ (38. 62 ± 5.52) ng／L vs .(31. 01 ± 5. 01) ng／L] in combined treatment group , P＝0.001 both .There were no significant difference in incidence of adverse reactions between two groups , P＞ 0.05 all.Conclusion : Valsartan combined hydrochlorothiazide can effectively improve levels of 24hDBP ,24hSBP and blood pressure variability ,and contrib— ute to regulating NO and ET levels in patients with hypertension .
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of isoliquiritigenin on the migration and invasion of human glioma stem cells and the underlying mechanism.</p><p><b>METHODS</b>The stem cell markers CD133 and Nestin in SHG44 human glioma stem cells were examined with immunofluorescence microscopy. The migration and invasion ability of glioma stem cells was determined by transwell method. The mRNA and protein expression of matrix metalloproteinase (MMP)-2 and MMP-9 were detected by real-time RT-PCR and Western blot, respectively.</p><p><b>RESULTS</b>CD133 and Nestin were positive in SHG44 cells. The number of migrated cells in SHG44 cells treated with 20 and 80 μmol/L isoliquiritigenin for 48 h were significantly lower than that in control group (76±5 and 42±4 vs. 85±6, all <0.01), and the number of migrated cells in 80 μmol/L isoliquiritigenin group was lower than that in 20 μmol/L isoliquiritigenin group (<0.01). The numbers of cells crossing through membrane in 20 and 80 μmol/L isoliquiritigenin groups were 190±13 and 130±9, respectively, which were significantly lower than that in control group (230±14, all <0.01), and the number of crossed cells in the 80 μmol/L isoliquiritigenin group was lower than that in 20 μmol/L isoliquiritigenin group (<0.01). The mRNA and protein expression levels of MMP-2 and MMP-9 were decreased compared with control group (<0.05 or <0.01), and the expression levels in 80 μmol/L isoliquiritigenin group were lower than those in 20 μmol/L isoliquiritigenin group (<0.05 or <0.01).</p><p><b>CONCLUSIONS</b>Isoliquiritigenin exhibits antitumor effects on glioma stem cells by inhibiting cell migration and invasion, which may be related to the down-regulation of MMP-2 and MMP-9.</p>
Subject(s)
Humans , Cell Line, Tumor , Cell Movement , Chalcones , Down-Regulation , Glioma , Matrix Metalloproteinase 2 , Matrix Metalloproteinase 9 , Neoplasm Invasiveness , Neoplastic Stem Cells , RNA, MessengerABSTRACT
Extracellular vesicles (EVs) are vesicles like body of phospholipid bilayer membrane, which are able to mediate the transfer of genetic material. There are receptors, proteins and nucleic acids in the body, with carrying tumor genetic material, regulating tumor microenvironment, promoting tumor angiogenesis and mediating tumor cell metastasis. At present, it is found that there is a close re-lationship between the secretion and the metastasis of lung cancer. The main aspects of the brain metastasis of the lung cancer medi-ated by the exocrine body include the regulation of the microenvironment of the brain, the destruction of the blood-brain barrier, and the regulation of tumor cell pathology. The study on the relationship between the tumor and the metastasis of lung cancer may pro-vide more molecular targets for the development, diagnosis and treatment of lung cancer.