ABSTRACT
Objective To study the relationship between serum levels of C reactive protein,adiponectin and severity of disease,joint function in patients with rheumatoid arthritis(RA).Methods A total of 85 cases of RA treated in our hospital from September 2014 to August 2016 were selected into the RA group,and 20 healthy persons were recruited into control group.The serum C reactive protein,adiponectin were detected,compared in the two groups,and to investigate the relationship between them with severity of disease,joint function of the patients.Results C reactive protein of RA patients was(23.63±5.36) pg/mL,which was significant higher than that of the control group,the adiponectin,the ratio of C reactive protein and adiponectin were(4.64±1.74)ng/mL,0.37±0.06 respectively,which were significant lower than those of the control group,the differences were statistically significant(P<0.05).RA patients with more serious joint injury and disease status,the adiponectin,the ratio of adiponectin and C reactive protien were more lower,the C reactive protien was more higher,the differences in different joint injury and disease status groups were significant(P<0.05).Logistic analysis showed that adiponectin(P=0.04),the ratio of adiponectin and C reactive protien(P=0.01) were independent risk factors of bone damage in RA patients.Conclusion Serum C reactive protein and adiponectin level were closely related to the severity of RA and joint function.
ABSTRACT
Background: Epigenetic mechanisms of gene transcription, including inactivation of tumor suppressor genes by hypermethylation and activation of oncogenes by hypomethylation, have been shown to contribute to breast tumorigenesis. Phosphatase and tensin homolog deleted on chromosome ten (PTEN) and Spleen tyrosine kinase (SYK) are both tumor suppressor genes and survivin is a novel member of the inhibitor of apoptosis (IAP) family which may promote tumorigenesis.Objective: To investigate the methylation status of PTEN, SYK, and survivin genes in breast cancer derived from Chinese women.Materials and methods: We examined the methylation status of these three genes in 52 paraffin-embedded breast cancer tissues using methylation-specific PCR (MSP) assay in conjunction with sequencing analysis.Results: PTEN and SYK were both methylated in 15.4% (8/52) of breast tumor tissues, while the survivin gene was all demethylated in the examined samples. Random selection of MSP products sequence analyses to three genes all revealed a homogenous methylation status in the CpG sites.Conclusion: The present study showed a moderate methylation status of PTEN and SYK, and an unmethylation status of survivin in breast tumor tissues derived from Chinese women, which suggested methylation mechanisms might be involved in the aberrant expression of these three genes in breast cancer development.