ABSTRACT
Objective: To investigate the expression of microRNA-154-3p (miR-154-3p) in non-small cell lung cancer (NSCLC) tissues and cell lines, and explore the role of miR-154-3p in metastasis process of NSCLC. Methods: The expression levels of miR-154-3p in NSCLC tissues and the corresponding paracarcinoma tissues, metastatic and non-metastatic NSCLC tissues, NSCLC cell lines (A549, H292, H1975, 95D and SPC-A-1) and human embryo lung WI38 cells were detected by realtime fluorogenic quantitative-PCR (RFQ-PCR). The miR-154-3p-negative control (miR-154-3p-NC, as a negative control) or miR-154-3p-mimics was transiently transfected into A549 and H292 cells by liposome. The effects of miR-154-3p on migration and invasion abilities of NSCLC A549 and H292 cells were determined by wound healing assay and Transwell invasion assay. The expressions of epithelial-mesenchymal transition (EMT)-related proteins E-cadherin, zonula occludens-1 (ZO-1) (epithelial marker), N-cadherin and vimentin (mesenchymal marker) induced by miR-154-3p overexpression were detected by Western blotting. The bioinformatic softwares were used to predict the potential targets of miR-1 54-3p, and the interested target genes were further validated by Dual-Luciferase Reporter Assay and Western blotting. Results: The expression levels of miR-154-3p were significantly lower in NSCLC tissues (P < 0.001), metastatic NSCLC tissues (P < 0.05) and NSCLC cell lines (P < 0.01) than those in their corresponding controls. Overexpression of miR-154-3p attenuated the migration and invasion abilities of both A549 and H292 cells (P < 0.05). The expressions of E-cadherin and ZO-1 proteins were up-regulated in A549 and H292 cells (all P < 0.05), whereas the vimentin expression in A549 cells and N-cadherin expression in H292 cells were down-regulated (both P < 0.05). Dual-Luciferase Reporter Assay and Western blotting results revealed that miR-154-3p could negatively regulate the expression of BMI1. Conclusion: MiR-154-3p is lowly expressed in NSCLC tissues and cell lines. The overexpression of miR-154-3p can inhibit the migration and invasion abilities of NSCLC cells and block the EMT progression. BMI1 may be one target gene of miR-154-3p.
ABSTRACT
<p><b>OBJECTIVES</b>To investigate the detection rate of small hepatocellular carcinomas using 3.0T MRI 3D-volumetric interpolated breath-hold examination (VIBE) sequence dynamic contrast-enhanced scan, and to evaluate its application in the diagnosis of small hepatocellular carcinomas.</p><p><b>METHODS</b>Imaging data of 61 cases of small hepatocellular carcinomas obtained using a 3.0 T Siemens Magnetom Trio Tim magnetic resonance scanner for plain scan and multi-phase dynamic contrast-enhanced scans of three-dimensional volumetric interpolated breath-hold examination (3D-VIBE) were retrospectively analyzed with reference to their pathological diagnoses. The signal and enhanced features and the detection rates of the tumors in different phases of examination were analyzed.</p><p><b>RESULTS</b>Sixty-six lesions were detected in the livers of the 61 patients by pathological examinations, of which 49 had pseudocapsule formation. In their T1WI plain scanning, 43 (65.15%) lesions were found, of which pseudocapsules were found in 19 (38.78%). In their T2WI imaging, 53 (80.30%) lesions were found. In dynamic enhanced 3D-VIBE sequence, 65 (98.48%) lesions were found, of which 35 (71.43%) were found with pseudocapsules. The sizes of the carcinomas ranged from 0.8 to 3.0 cm, 2.1cm on the average. The results of statistical analysis for the detection rates of the cancers of the three different sequences were significantly different (chi2=24.197, P less than 0.05). The differences of qualitative accuracy obtained by plain scan (T1WI + T2WI) and 3D-VIBE sequence were significantly different (chi2=66, P less than 0.05).</p><p><b>CONCLUSION</b>3.0 T MRI 3D-VIBE sequence dynamic contrast-enhanced scan is high-speed and can clearly demonstrate the arterial phase, the portal venous phase and the parenchymal phase images. It not only can find small lesions which can not be found with a plain scan, but also can assist diagnosis through a time-intensity curve. It is of great value for detection and differential diagnosis of small hepatocellular carcinomas.</p>
Subject(s)
Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Young Adult , Carcinoma, Hepatocellular , Diagnosis , Pathology , Imaging, Three-Dimensional , Liver , Pathology , Liver Neoplasms , Diagnosis , Pathology , Magnetic Resonance Imaging , MethodsABSTRACT
Objective To investigate flow patterns at carotid bifurcation in vivo by combining computational fluid dynamics(CFD)and MR angiography imaging.Methods Seven subjects underwent contrast-enhanced MR angiography of carotid artery in Siemens 3.0 T MR.Flow patterns of the carotid artery bifurcation were calculated and visualized by combining MR vascular imaging post-processing and CFD. Results The flow patterns of the carotid bifurcations in 7 subjects were varied with different phases of a cardiac cycle.The turbulent flow and back flow occurred at bifurcation and proximal of internal carotid artery (ICA)and external carotid artery(ECA),their occurrence and conformation were varied with different phase of a cardiac cycle.The turbulent flow and back flow faded out quickly when the blood flow to the distal of ICA and ECA.Conclusion CFD combined with MR angiography can be utilized to visualize the cyclical change of flow patterns of carotid bifurcation with different phases of a cardiac cycle.