Characteristics of blood type irregular antibodies in Han population of Chinese Sichuan area / 中国实验血液学杂志

<p><b>OBJECTIVE</b>To analyze the distribution of irregular antibody of red blood cells in Han population of Chinese Sichuan area, so as to provide valuable information for the safety of transfusion and decrease of immune hemolytic transfusion reaction.</p><p><b>METHODS</b>Blood samples from June 2006 to May 2013 were tested for irregular antibody screening and identification, calculating the composition rate, group characteristics and the positive detection rate of irregular antibody.</p><p><b>RESULTS</b>A total of 36287 blood samples were tested, out of them 571 samples were the irregular antibody positive, the positive rate was 1.574%(571/36 287), specific alloantibodies were found in 312 samples, the positive rate was 0.860%(312/36287). And autoantibody was found in 259 samples, the positive rate was 0.714%(259/36 287). The specific alloantibodies ratio in Rh system was the highest, reaching to 73.72%(230/312) with the positive rate of 0.634%;36 cases in Lewis system, account for 11.54%(36/312) with the positive rate of 0.099%; 34 cases in MNS system account for 10.89%(34/312) with the positive rate of 0.094%; direct coomb test showed positive result in 284 samples, the rate was 0.78%. The detected rate of positive irregular antibody in female is obviously higher than that in male patients (P<0.001), and it is also higher in people with pregnancy or transfusion than that in those without it (P<0.05).</p><p><b>CONCLUSION</b>The irregular antibody screening and identification are very important in blood transfusion, especially for female and people with transfusion or pregnant history.</p>

Influence on costal cartilage reparative regeneration by replanting the small blocks of autogeneic cartilage / 中华整形外科杂志

<p><b>OBJECTIVE</b>To investigate the influence on costal cartilage reparative regeneration by replanting the small blocks of autogeneic cartilage into the perichondrial pocket at the donor-site.</p><p><b>METHODS</b>16 rabbits (8-10 weeks old, 1.8-2.2 kg) were randomly divided into four groups as three experimental groups and one control group. The 1.5 cm in length of costal cartilage defect was made in experimental groups with the perichondrium and costochondral junction left completely intact. The cartilage defect was closed by 3 methods as saturation directly, or replanting the small blocks of autogeneic cartilage, or plugging bio-protein jelly after cartilage replanting. Each experimental group was handled with two methods in two sides of costal cartilage. No operation was performed in control group. All the rabbits were sacrificed 16 weeks after operation. The appearance of thoracic cage and new-formed tissue at the defect site were examined grossly. Haematoxylin-eosin staining was performed to evaluate the characteristics of new-formed tissues and biomechanical detection was used to measure intension of new-formed tissues.</p><p><b>RESULTS</b>The appearance of thoracic cage was normal in every experimental group. Histological study showed that the defect was filled with abundant fibrous tissue in each group. The chipping of cartilage survived effectively with little proliferation. Biomechanical detection showed that the intension of new-formed tissue in the non-replanted group [(193.92 +/- 41.41) N] was obviously less than that in the replanted group [(318.88 +/- 28.28) N], or bio-protein jelly group [(301.00 +/- 39.52) N], or control group [(300.54 +/- 38.35) N] (P < 0.01). Furthermore, there was no statistical difference between the latter three groups (P > 0.05).</p><p><b>CONCLUSIONS</b>Although replanting the chipping of cartilage can't promote reparative regeneration of hyaline cartilage, it can definitively strengthen the intensity of new-formed tissue, reinforce thoracic stability. It may also indirectly decrease the incidence rate of postoperative chest wall deformity.</p>